Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Epidemiologia e Controle de Infecção |
Texto Completo: | https://online.unisc.br/seer/index.php/epidemiologia/article/view/8200 |
Resumo: | Justificative and Objectives: the study aimed the assessment of the antibacterial activity of crude extracts and fractions of Ibicella lutea, front Staphylococcus aureus, thecombination of these compounds and cytotoxic activity. Methods: was used for antibacterial activity the Microdilution Test Broth, and performed the Checkerboard Test. The extracts showed antibacterial activity were submitted to the citotoxicity test, with macrophages cell and determination of the Selectivity Index (SI). Results: The acetate etila fraction (AcOE) was better antimicrobial activity (6.25 µg/mL) compared with the others extracts and fractions used, however none of the compounds showed bactericidal activity in concentrations employed. In present study, we can be observed an additive activity between AcOE and methanolic (MeOH) fractions, and indifferent interaction between crude extracts. According to citotoxicity test, the extract which led to a higher survival rate of macrophage cells was the fraction AcOE (IC50%=30.35 µg/mL). However, when the calculated SI no satisfactory results (SI < 10) to any of the extracts was observed. Conclusions: in the present study we can observe an antimicrobial activity of the fractions AcOE and MeOH to S. aureus, as well as an additive this potential when the fractions are combined, providing support from isolation and characterization of yours active components. Despite the extracts did not showed a satisfactory SI, new toxicity studies should be performed to establish the potential use of safety with the products derived from I. lutea, such as drugs for topical and biocide products. |
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Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureusAvaliação de Ibicella lutea como agente antimicrobiano frente à Staphylococcus aureusJustificative and Objectives: the study aimed the assessment of the antibacterial activity of crude extracts and fractions of Ibicella lutea, front Staphylococcus aureus, thecombination of these compounds and cytotoxic activity. Methods: was used for antibacterial activity the Microdilution Test Broth, and performed the Checkerboard Test. The extracts showed antibacterial activity were submitted to the citotoxicity test, with macrophages cell and determination of the Selectivity Index (SI). Results: The acetate etila fraction (AcOE) was better antimicrobial activity (6.25 µg/mL) compared with the others extracts and fractions used, however none of the compounds showed bactericidal activity in concentrations employed. In present study, we can be observed an additive activity between AcOE and methanolic (MeOH) fractions, and indifferent interaction between crude extracts. According to citotoxicity test, the extract which led to a higher survival rate of macrophage cells was the fraction AcOE (IC50%=30.35 µg/mL). However, when the calculated SI no satisfactory results (SI < 10) to any of the extracts was observed. Conclusions: in the present study we can observe an antimicrobial activity of the fractions AcOE and MeOH to S. aureus, as well as an additive this potential when the fractions are combined, providing support from isolation and characterization of yours active components. Despite the extracts did not showed a satisfactory SI, new toxicity studies should be performed to establish the potential use of safety with the products derived from I. lutea, such as drugs for topical and biocide products.Justificativa e Objetivos: O estudo objetivou a avaliação da atividade antibacteriana de extratos brutos e frações de Ibicella lutea, na inibição do crescimento de Staphylococcus aureus, avaliando a combinação destes compostos e sua atividade citotóxica. Métodos: Para a atividade antibacteriana dos extratos foi utilizado o Teste de Microdiluição em Caldo, seguido do Teste Checkerboard. Os extratos que apresentaram atividade antibacteriana foram submetidos ao teste de citotoxicidade, com células macrofágicas e determinação do seu Índice de Seletividade (IS). Resultados: A fração de acetato de etila(AcOE) obteve o melhor potencial antibacteriano (6,25 µg/mL), entretanto nenhum dos compostos testados apresentaram atividade bactericida nas concentrações empregadas. Neste estudo pode-se observar uma ação aditiva entre as frações AcOE e metanólica (MeOH), sendo a interação entre os extratos brutos indiferente. De acordo com o teste de citotoxicidade, a fração AcOE apresentou uma maior índice de sobrevivência das células macrofágicas (IC50%=30,35 µg/mL). Entretanto, quando calculado o IS, não houve resultados satisfatórios (IS < 10) para os extratos analisados. Conclusões: Neste estudo observou-se o potencial antibacteriano das frações AcOE e MeOH, assim como uma ação aditiva na combinação das frações, dando suporte para o isolamento e caracterização de seus componentes ativos. Apesar dos extratos não apresentarem um IS satisfatório, novos estudos de toxicidade devem ser realizados para determinar com segurança o potencial de uso dos produtos provenientes de I. lutea, como é o caso de medicamentos para uso tópico ou biocidas.Unisc2016-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/820010.17058/reci.v6i4.8200Revista de Epidemiologia e Controle de Infecção; Vol. 6 No. 4 (2016); 191-196Revista de Epidemiologia e Controle de Infecção; v. 6 n. 4 (2016); 191-1962238-3360reponame:Revista de Epidemiologia e Controle de Infecçãoinstname:Universidade de Santa Cruz do Sul (UNISC)instacron:UNISCporhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/8200/5180Copyright (c) 2016 Revista de Epidemiologia e Controle de Infecçãoinfo:eu-repo/semantics/openAccessVolcão, Lisiane MartinsCoelho, Tatiane SilveiraVàsquez, AlvároCerdeiras, Maria PiaSilva, Pedro Eduardo Almeida daJúnior, Flávio Manoel Rodrigues da SilvaRamos, Daniela Fernandes2019-01-21T12:53:32Zoai:ojs.online.unisc.br:article/8200Revistahttps://online.unisc.br/seer/index.php/epidemiologia/indexONGhttp://online.unisc.br/seer/index.php/epidemiologia/oai||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com2238-33602238-3360opendoar:2019-01-21T12:53:32Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC)false |
dc.title.none.fl_str_mv |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus Avaliação de Ibicella lutea como agente antimicrobiano frente à Staphylococcus aureus |
title |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
spellingShingle |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus Volcão, Lisiane Martins |
title_short |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
title_full |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
title_fullStr |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
title_full_unstemmed |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
title_sort |
Assessment of Ibicella lutea for antibacterial agent front Staphylococcus aureus |
author |
Volcão, Lisiane Martins |
author_facet |
Volcão, Lisiane Martins Coelho, Tatiane Silveira Vàsquez, Alváro Cerdeiras, Maria Pia Silva, Pedro Eduardo Almeida da Júnior, Flávio Manoel Rodrigues da Silva Ramos, Daniela Fernandes |
author_role |
author |
author2 |
Coelho, Tatiane Silveira Vàsquez, Alváro Cerdeiras, Maria Pia Silva, Pedro Eduardo Almeida da Júnior, Flávio Manoel Rodrigues da Silva Ramos, Daniela Fernandes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Volcão, Lisiane Martins Coelho, Tatiane Silveira Vàsquez, Alváro Cerdeiras, Maria Pia Silva, Pedro Eduardo Almeida da Júnior, Flávio Manoel Rodrigues da Silva Ramos, Daniela Fernandes |
description |
Justificative and Objectives: the study aimed the assessment of the antibacterial activity of crude extracts and fractions of Ibicella lutea, front Staphylococcus aureus, thecombination of these compounds and cytotoxic activity. Methods: was used for antibacterial activity the Microdilution Test Broth, and performed the Checkerboard Test. The extracts showed antibacterial activity were submitted to the citotoxicity test, with macrophages cell and determination of the Selectivity Index (SI). Results: The acetate etila fraction (AcOE) was better antimicrobial activity (6.25 µg/mL) compared with the others extracts and fractions used, however none of the compounds showed bactericidal activity in concentrations employed. In present study, we can be observed an additive activity between AcOE and methanolic (MeOH) fractions, and indifferent interaction between crude extracts. According to citotoxicity test, the extract which led to a higher survival rate of macrophage cells was the fraction AcOE (IC50%=30.35 µg/mL). However, when the calculated SI no satisfactory results (SI < 10) to any of the extracts was observed. Conclusions: in the present study we can observe an antimicrobial activity of the fractions AcOE and MeOH to S. aureus, as well as an additive this potential when the fractions are combined, providing support from isolation and characterization of yours active components. Despite the extracts did not showed a satisfactory SI, new toxicity studies should be performed to establish the potential use of safety with the products derived from I. lutea, such as drugs for topical and biocide products. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/8200 10.17058/reci.v6i4.8200 |
url |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/8200 |
identifier_str_mv |
10.17058/reci.v6i4.8200 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/8200/5180 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Revista de Epidemiologia e Controle de Infecção info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Revista de Epidemiologia e Controle de Infecção |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Unisc |
publisher.none.fl_str_mv |
Unisc |
dc.source.none.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção; Vol. 6 No. 4 (2016); 191-196 Revista de Epidemiologia e Controle de Infecção; v. 6 n. 4 (2016); 191-196 2238-3360 reponame:Revista de Epidemiologia e Controle de Infecção instname:Universidade de Santa Cruz do Sul (UNISC) instacron:UNISC |
instname_str |
Universidade de Santa Cruz do Sul (UNISC) |
instacron_str |
UNISC |
institution |
UNISC |
reponame_str |
Revista de Epidemiologia e Controle de Infecção |
collection |
Revista de Epidemiologia e Controle de Infecção |
repository.name.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC) |
repository.mail.fl_str_mv |
||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com |
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1800218810631847936 |