Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Epidemiologia e Controle de Infecção |
Texto Completo: | https://online.unisc.br/seer/index.php/epidemiologia/article/view/15303 |
Resumo: | Justification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characterize the isolates of the genus Staphylococcus spp. from bloodstream infections as to the production of bacterial biofilm and resistance to the main antimicrobials used in clinical practice. Methods: Blood cultures were collected with an indication of positivity for bacterial growth from multiple sectors of the study hospital, which were subsequently processed to identify the bacterial genus through the use of phenotypic tests for Gram positive bacteria. The verification of the resistance profile was performed following the Kirby-Bauer disk diffusion. The identification of the production and quantification of the bacterial biofilm occurred following the protocol described by O’toole (2010). Results: The most frequent clinical isolate was Coagulase negative Staphylococci 38 (54.29%), followed by Staphylococcus aureus 32 (45.71%). Resistance to erythromycin, norfloxacin, levofloxacin and azithromycin was observed in most isolates (70%). Regarding methicillin, more MRSA (59.38%) than MR-CONS (47.37%) were isolated. The ICU was the place where the formation of the biofilm showed indicative data of greater adherence, which was associated with MRSA strains. Conclusion: The bacterial isolates associated with bloodstream infections showed high resistance to antimicrobials. The presence of MRSA and MR-CONS with strong and/or moderate biofilm production capacity represents a greater risk to the health of patients affected by infections caused by these agents. |
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Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobialsBiofilmesHemoculturaStaphylococcusJustification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characterize the isolates of the genus Staphylococcus spp. from bloodstream infections as to the production of bacterial biofilm and resistance to the main antimicrobials used in clinical practice. Methods: Blood cultures were collected with an indication of positivity for bacterial growth from multiple sectors of the study hospital, which were subsequently processed to identify the bacterial genus through the use of phenotypic tests for Gram positive bacteria. The verification of the resistance profile was performed following the Kirby-Bauer disk diffusion. The identification of the production and quantification of the bacterial biofilm occurred following the protocol described by O’toole (2010). Results: The most frequent clinical isolate was Coagulase negative Staphylococci 38 (54.29%), followed by Staphylococcus aureus 32 (45.71%). Resistance to erythromycin, norfloxacin, levofloxacin and azithromycin was observed in most isolates (70%). Regarding methicillin, more MRSA (59.38%) than MR-CONS (47.37%) were isolated. The ICU was the place where the formation of the biofilm showed indicative data of greater adherence, which was associated with MRSA strains. Conclusion: The bacterial isolates associated with bloodstream infections showed high resistance to antimicrobials. The presence of MRSA and MR-CONS with strong and/or moderate biofilm production capacity represents a greater risk to the health of patients affected by infections caused by these agents.Unisc2021-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/1530310.17058/reci.v11i2.15303Revista de Epidemiologia e Controle de Infecção; Vol. 11 No. 2 (2021)Revista de Epidemiologia e Controle de Infecção; v. 11 n. 2 (2021)2238-3360reponame:Revista de Epidemiologia e Controle de Infecçãoinstname:Universidade de Santa Cruz do Sul (UNISC)instacron:UNISCenghttps://online.unisc.br/seer/index.php/epidemiologia/article/view/15303/9962Copyright (c) 2021 Lamartine Rodrigues Martins, Mariana Quitéria de Morais Silva, Carlos Alberto Medeiros Neto, Maria Izabelly Silva Pimentel, Igor Vasconcelos Rocha, Sibele Ribeiro de Oliveirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMartins, Lamartine RodriguesSilva, Mariana Quitéria de MoraisMedeiros Neto, Carlos AlbertoPimentel, Maria Izabelly SilvaRocha, Igor VasconcelosOliveira, Sibele Ribeiro de2022-08-08T13:09:10Zoai:ojs.online.unisc.br:article/15303Revistahttps://online.unisc.br/seer/index.php/epidemiologia/indexONGhttp://online.unisc.br/seer/index.php/epidemiologia/oai||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com2238-33602238-3360opendoar:2022-08-08T13:09:10Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC)false |
dc.title.none.fl_str_mv |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
title |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
spellingShingle |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials Martins, Lamartine Rodrigues Biofilmes Hemocultura Staphylococcus |
title_short |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
title_full |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
title_fullStr |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
title_full_unstemmed |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
title_sort |
Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials |
author |
Martins, Lamartine Rodrigues |
author_facet |
Martins, Lamartine Rodrigues Silva, Mariana Quitéria de Morais Medeiros Neto, Carlos Alberto Pimentel, Maria Izabelly Silva Rocha, Igor Vasconcelos Oliveira, Sibele Ribeiro de |
author_role |
author |
author2 |
Silva, Mariana Quitéria de Morais Medeiros Neto, Carlos Alberto Pimentel, Maria Izabelly Silva Rocha, Igor Vasconcelos Oliveira, Sibele Ribeiro de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Martins, Lamartine Rodrigues Silva, Mariana Quitéria de Morais Medeiros Neto, Carlos Alberto Pimentel, Maria Izabelly Silva Rocha, Igor Vasconcelos Oliveira, Sibele Ribeiro de |
dc.subject.por.fl_str_mv |
Biofilmes Hemocultura Staphylococcus |
topic |
Biofilmes Hemocultura Staphylococcus |
description |
Justification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characterize the isolates of the genus Staphylococcus spp. from bloodstream infections as to the production of bacterial biofilm and resistance to the main antimicrobials used in clinical practice. Methods: Blood cultures were collected with an indication of positivity for bacterial growth from multiple sectors of the study hospital, which were subsequently processed to identify the bacterial genus through the use of phenotypic tests for Gram positive bacteria. The verification of the resistance profile was performed following the Kirby-Bauer disk diffusion. The identification of the production and quantification of the bacterial biofilm occurred following the protocol described by O’toole (2010). Results: The most frequent clinical isolate was Coagulase negative Staphylococci 38 (54.29%), followed by Staphylococcus aureus 32 (45.71%). Resistance to erythromycin, norfloxacin, levofloxacin and azithromycin was observed in most isolates (70%). Regarding methicillin, more MRSA (59.38%) than MR-CONS (47.37%) were isolated. The ICU was the place where the formation of the biofilm showed indicative data of greater adherence, which was associated with MRSA strains. Conclusion: The bacterial isolates associated with bloodstream infections showed high resistance to antimicrobials. The presence of MRSA and MR-CONS with strong and/or moderate biofilm production capacity represents a greater risk to the health of patients affected by infections caused by these agents. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/15303 10.17058/reci.v11i2.15303 |
url |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/15303 |
identifier_str_mv |
10.17058/reci.v11i2.15303 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/15303/9962 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Unisc |
publisher.none.fl_str_mv |
Unisc |
dc.source.none.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção; Vol. 11 No. 2 (2021) Revista de Epidemiologia e Controle de Infecção; v. 11 n. 2 (2021) 2238-3360 reponame:Revista de Epidemiologia e Controle de Infecção instname:Universidade de Santa Cruz do Sul (UNISC) instacron:UNISC |
instname_str |
Universidade de Santa Cruz do Sul (UNISC) |
instacron_str |
UNISC |
institution |
UNISC |
reponame_str |
Revista de Epidemiologia e Controle de Infecção |
collection |
Revista de Epidemiologia e Controle de Infecção |
repository.name.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC) |
repository.mail.fl_str_mv |
||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com |
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1800218811831418880 |