Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate

Detalhes bibliográficos
Autor(a) principal: De Gregorio, Lara S. [UNESP]
Data de Publicação: 2021
Outros Autores: Franco-Belussi, Lilian [UNESP], De Oliveira, Classius [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2994/SAJH-D-19-00111.1
http://hdl.handle.net/11449/229467
Resumo: Abstract. Amphibians are threatened with extinction worldwide, and one of the global causes is environmental pollution. Several toxicants can interfere with their immune system, making them more susceptible to disease, which also contributes to their decline. The contaminants 4-nonylphenol (NP), a detergent with estrogenic properties, and cyproterone acetate (CPA), an anti-androgenic medicine, are known to impair the immune system of some vertebrates, but the effects are understudied in anurans. In this study, we evaluated the effects of NP (at 1, 10, and 100 μg/L) and CPA (at 0.025, 0.25, and 2.5 ng/L) on leukocyte count in tadpoles and juveniles of Lithobates catesbeianus. We analyzed lymphocytes, neutrophils, basophils, eosinophils, monocytes, and thrombocytes in a proportional count and made comparisons between the groups. The experiments lasted 28 d, and the conditions were almost identical for both stages (varying only the volume of water). The tadpoles showed decreased lymphocytes and increased neutrophils at 1 μg/L NP and increased eosinophils at the two highest doses of CPA. The juveniles showed reduced neutrophils in all treatments with both compounds and increased thrombocytes (except at 2.5 ng/L CPA). Also, all dosages of CPA increased basophils. Although the life-stages had different response patterns, our study showed that NP and CPA altered the proportion of leukocytes on blood stream in both stages, probably as a response against the negative effects of the compounds, which might make the animals more susceptible to diseases and contribute to their decline.
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spelling Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone AcetateEcotoxicologyEndocrine disrupting chemicalsLeukocytesPollutantsAbstract. Amphibians are threatened with extinction worldwide, and one of the global causes is environmental pollution. Several toxicants can interfere with their immune system, making them more susceptible to disease, which also contributes to their decline. The contaminants 4-nonylphenol (NP), a detergent with estrogenic properties, and cyproterone acetate (CPA), an anti-androgenic medicine, are known to impair the immune system of some vertebrates, but the effects are understudied in anurans. In this study, we evaluated the effects of NP (at 1, 10, and 100 μg/L) and CPA (at 0.025, 0.25, and 2.5 ng/L) on leukocyte count in tadpoles and juveniles of Lithobates catesbeianus. We analyzed lymphocytes, neutrophils, basophils, eosinophils, monocytes, and thrombocytes in a proportional count and made comparisons between the groups. The experiments lasted 28 d, and the conditions were almost identical for both stages (varying only the volume of water). The tadpoles showed decreased lymphocytes and increased neutrophils at 1 μg/L NP and increased eosinophils at the two highest doses of CPA. The juveniles showed reduced neutrophils in all treatments with both compounds and increased thrombocytes (except at 2.5 ng/L CPA). Also, all dosages of CPA increased basophils. Although the life-stages had different response patterns, our study showed that NP and CPA altered the proportion of leukocytes on blood stream in both stages, probably as a response against the negative effects of the compounds, which might make the animals more susceptible to diseases and contribute to their decline.Department of Biology São Paulo State University, CEP 15054-000, São José do Rio PretoLaboratory of Experimental Pathology Federal University of Mato Grosso Do sul Institute of Biosciences, CEP 79002-970, Campo GrandeDepartment of Biology São Paulo State University, CEP 15054-000, São José do Rio PretoUniversidade Estadual Paulista (UNESP)Institute of BiosciencesDe Gregorio, Lara S. [UNESP]Franco-Belussi, Lilian [UNESP]De Oliveira, Classius [UNESP]2022-04-29T08:32:41Z2022-04-29T08:32:41Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article75-83http://dx.doi.org/10.2994/SAJH-D-19-00111.1South American Journal of Herpetology, v. 20, n. 1, p. 75-83, 2021.1982-355X1808-9798http://hdl.handle.net/11449/22946710.2994/SAJH-D-19-00111.12-s2.0-85114369950Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSouth American Journal of Herpetologyinfo:eu-repo/semantics/openAccess2022-04-29T08:32:41Zoai:repositorio.unesp.br:11449/229467Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:25:24.228548Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
title Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
spellingShingle Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
De Gregorio, Lara S. [UNESP]
Ecotoxicology
Endocrine disrupting chemicals
Leukocytes
Pollutants
title_short Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
title_full Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
title_fullStr Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
title_full_unstemmed Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
title_sort Leukocyte Profile of Tadpoles and Juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the Effects of Nonylphenol and Cyproterone Acetate
author De Gregorio, Lara S. [UNESP]
author_facet De Gregorio, Lara S. [UNESP]
Franco-Belussi, Lilian [UNESP]
De Oliveira, Classius [UNESP]
author_role author
author2 Franco-Belussi, Lilian [UNESP]
De Oliveira, Classius [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Institute of Biosciences
dc.contributor.author.fl_str_mv De Gregorio, Lara S. [UNESP]
Franco-Belussi, Lilian [UNESP]
De Oliveira, Classius [UNESP]
dc.subject.por.fl_str_mv Ecotoxicology
Endocrine disrupting chemicals
Leukocytes
Pollutants
topic Ecotoxicology
Endocrine disrupting chemicals
Leukocytes
Pollutants
description Abstract. Amphibians are threatened with extinction worldwide, and one of the global causes is environmental pollution. Several toxicants can interfere with their immune system, making them more susceptible to disease, which also contributes to their decline. The contaminants 4-nonylphenol (NP), a detergent with estrogenic properties, and cyproterone acetate (CPA), an anti-androgenic medicine, are known to impair the immune system of some vertebrates, but the effects are understudied in anurans. In this study, we evaluated the effects of NP (at 1, 10, and 100 μg/L) and CPA (at 0.025, 0.25, and 2.5 ng/L) on leukocyte count in tadpoles and juveniles of Lithobates catesbeianus. We analyzed lymphocytes, neutrophils, basophils, eosinophils, monocytes, and thrombocytes in a proportional count and made comparisons between the groups. The experiments lasted 28 d, and the conditions were almost identical for both stages (varying only the volume of water). The tadpoles showed decreased lymphocytes and increased neutrophils at 1 μg/L NP and increased eosinophils at the two highest doses of CPA. The juveniles showed reduced neutrophils in all treatments with both compounds and increased thrombocytes (except at 2.5 ng/L CPA). Also, all dosages of CPA increased basophils. Although the life-stages had different response patterns, our study showed that NP and CPA altered the proportion of leukocytes on blood stream in both stages, probably as a response against the negative effects of the compounds, which might make the animals more susceptible to diseases and contribute to their decline.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
2022-04-29T08:32:41Z
2022-04-29T08:32:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2994/SAJH-D-19-00111.1
South American Journal of Herpetology, v. 20, n. 1, p. 75-83, 2021.
1982-355X
1808-9798
http://hdl.handle.net/11449/229467
10.2994/SAJH-D-19-00111.1
2-s2.0-85114369950
url http://dx.doi.org/10.2994/SAJH-D-19-00111.1
http://hdl.handle.net/11449/229467
identifier_str_mv South American Journal of Herpetology, v. 20, n. 1, p. 75-83, 2021.
1982-355X
1808-9798
10.2994/SAJH-D-19-00111.1
2-s2.0-85114369950
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv South American Journal of Herpetology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 75-83
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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