Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2013.03.007 http://hdl.handle.net/11449/75520 |
Resumo: | The objective of this experiment was to evaluate the effects of bovine somatotropin administration on serum concentrations of glucose, insulin, NEFA, IGF-I, and progesterone (P4) in ovariectomized non-lactating dairy cows receiving exogenous P4, as a model to estimate treatment effects on hepatic P4 degradation. Ten non-lactating, non-pregnant, and ovariectomized Gir×Holstein cows were assigned to the experiment (d -14 to 27). On d 0, cows were ranked by BW and BCS, and randomly assigned to one of two treatments: (1) bovine somatotropin (BST; n=5) or (2) saline control (control; n=5). Cows assigned to the BST treatment were administered s.c. injections containing 500. mg of sometribove zinc on d 0, 9, and 18 of the experiment, whereas control cows concurrently received a 10-mL s.c. injection of 0.9% saline. On d -2, cows were inserted with an intravaginal releasing device containing 1.9. g of P4, which remained in the cows until the end the experiment (d 27). Cow BW and BCS were assessed on d -14, 0, and 27. Blood samples were collected daily from d 0 to d 27, at 0 (immediately before), 1, and 2. h relative to concentrate feeding for determination of serum glucose, insulin, NEFA, P4, and IGF-I concentrations. Concentrations of glucose, NEFA, and insulin obtained prior to feeding (0. h) were used to determine pre-prandial revised quantitative insulin sensitivity check index (RQUICKI). No treatment effects were detected for BW (P=0.72) and BCS change (P=0.79) during the experiment. Beginning on d 2 of the experiment, BST cows had greater (P≤0.01) serum IGF-I concentrations compared with control cohorts (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.05) insulin concentrations compared with control cohorts from d 8 to d 11, d 16 and 17, as well as from d 19 to d 21 of the experiment (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.01) mean glucose and NEFA concentrations, as well as reduced (P<0.01) mean RQUICKI during the experiment compared with control cohorts. No treatment effects, however, were detected (P=0.73) for serum P4 concentrations. In conclusion, results from this experiment indicate that hepatic P4 catabolism is not directly regulated by circulating IGF-I, whereas BST administration decreases insulin sensitivity in non-lactating dairy cows in adequate nutritional status. © 2013 Elsevier B.V. |
id |
UNSP_00853168bd807e0c20e892020ab8d961 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/75520 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cowsDairy cowsIGF-IInsulinProgesteroneBosBovinaeThe objective of this experiment was to evaluate the effects of bovine somatotropin administration on serum concentrations of glucose, insulin, NEFA, IGF-I, and progesterone (P4) in ovariectomized non-lactating dairy cows receiving exogenous P4, as a model to estimate treatment effects on hepatic P4 degradation. Ten non-lactating, non-pregnant, and ovariectomized Gir×Holstein cows were assigned to the experiment (d -14 to 27). On d 0, cows were ranked by BW and BCS, and randomly assigned to one of two treatments: (1) bovine somatotropin (BST; n=5) or (2) saline control (control; n=5). Cows assigned to the BST treatment were administered s.c. injections containing 500. mg of sometribove zinc on d 0, 9, and 18 of the experiment, whereas control cows concurrently received a 10-mL s.c. injection of 0.9% saline. On d -2, cows were inserted with an intravaginal releasing device containing 1.9. g of P4, which remained in the cows until the end the experiment (d 27). Cow BW and BCS were assessed on d -14, 0, and 27. Blood samples were collected daily from d 0 to d 27, at 0 (immediately before), 1, and 2. h relative to concentrate feeding for determination of serum glucose, insulin, NEFA, P4, and IGF-I concentrations. Concentrations of glucose, NEFA, and insulin obtained prior to feeding (0. h) were used to determine pre-prandial revised quantitative insulin sensitivity check index (RQUICKI). No treatment effects were detected for BW (P=0.72) and BCS change (P=0.79) during the experiment. Beginning on d 2 of the experiment, BST cows had greater (P≤0.01) serum IGF-I concentrations compared with control cohorts (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.05) insulin concentrations compared with control cohorts from d 8 to d 11, d 16 and 17, as well as from d 19 to d 21 of the experiment (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.01) mean glucose and NEFA concentrations, as well as reduced (P<0.01) mean RQUICKI during the experiment compared with control cohorts. No treatment effects, however, were detected (P=0.73) for serum P4 concentrations. In conclusion, results from this experiment indicate that hepatic P4 catabolism is not directly regulated by circulating IGF-I, whereas BST administration decreases insulin sensitivity in non-lactating dairy cows in adequate nutritional status. © 2013 Elsevier B.V.Departamento de Produção Animal Faculdade de Medicina Veterinária e Zootecnia UNESP, Botucatu, SP 18618-000Oregon State University Eastern Oregon Agricultural Research Center, Burns, ORDepartamento de Produção Animal Faculdade de Medicina Veterinária e Zootecnia UNESP, Botucatu, SP 18618-000Universidade Estadual Paulista (Unesp)Eastern Oregon Agricultural Research CenterAboin, A. C. [UNESP]Cooke, Reinaldo Fernandes [UNESP]Vieira, F. V R [UNESP]Leiva, T. [UNESP]Vasconcelos, José Luiz Moraes [UNESP]2014-05-27T11:29:35Z2014-05-27T11:29:35Z2013-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article240-245application/pdfhttp://dx.doi.org/10.1016/j.livsci.2013.03.007Livestock Science, v. 154, n. 1-3, p. 240-245, 2013.1871-1413http://hdl.handle.net/11449/7552010.1016/j.livsci.2013.03.007WOS:0003198522000332-s2.0-848771072662-s2.0-84877107266.pdf1069922096621313Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2023-10-13T06:04:30Zoai:repositorio.unesp.br:11449/75520Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-13T06:04:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
title |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
spellingShingle |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows Aboin, A. C. [UNESP] Dairy cows IGF-I Insulin Progesterone Bos Bovinae |
title_short |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
title_full |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
title_fullStr |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
title_full_unstemmed |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
title_sort |
Effects of bovine somatotropin injection on serum concentrations of progesterone in non-lactating dairy cows |
author |
Aboin, A. C. [UNESP] |
author_facet |
Aboin, A. C. [UNESP] Cooke, Reinaldo Fernandes [UNESP] Vieira, F. V R [UNESP] Leiva, T. [UNESP] Vasconcelos, José Luiz Moraes [UNESP] |
author_role |
author |
author2 |
Cooke, Reinaldo Fernandes [UNESP] Vieira, F. V R [UNESP] Leiva, T. [UNESP] Vasconcelos, José Luiz Moraes [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Eastern Oregon Agricultural Research Center |
dc.contributor.author.fl_str_mv |
Aboin, A. C. [UNESP] Cooke, Reinaldo Fernandes [UNESP] Vieira, F. V R [UNESP] Leiva, T. [UNESP] Vasconcelos, José Luiz Moraes [UNESP] |
dc.subject.por.fl_str_mv |
Dairy cows IGF-I Insulin Progesterone Bos Bovinae |
topic |
Dairy cows IGF-I Insulin Progesterone Bos Bovinae |
description |
The objective of this experiment was to evaluate the effects of bovine somatotropin administration on serum concentrations of glucose, insulin, NEFA, IGF-I, and progesterone (P4) in ovariectomized non-lactating dairy cows receiving exogenous P4, as a model to estimate treatment effects on hepatic P4 degradation. Ten non-lactating, non-pregnant, and ovariectomized Gir×Holstein cows were assigned to the experiment (d -14 to 27). On d 0, cows were ranked by BW and BCS, and randomly assigned to one of two treatments: (1) bovine somatotropin (BST; n=5) or (2) saline control (control; n=5). Cows assigned to the BST treatment were administered s.c. injections containing 500. mg of sometribove zinc on d 0, 9, and 18 of the experiment, whereas control cows concurrently received a 10-mL s.c. injection of 0.9% saline. On d -2, cows were inserted with an intravaginal releasing device containing 1.9. g of P4, which remained in the cows until the end the experiment (d 27). Cow BW and BCS were assessed on d -14, 0, and 27. Blood samples were collected daily from d 0 to d 27, at 0 (immediately before), 1, and 2. h relative to concentrate feeding for determination of serum glucose, insulin, NEFA, P4, and IGF-I concentrations. Concentrations of glucose, NEFA, and insulin obtained prior to feeding (0. h) were used to determine pre-prandial revised quantitative insulin sensitivity check index (RQUICKI). No treatment effects were detected for BW (P=0.72) and BCS change (P=0.79) during the experiment. Beginning on d 2 of the experiment, BST cows had greater (P≤0.01) serum IGF-I concentrations compared with control cohorts (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.05) insulin concentrations compared with control cohorts from d 8 to d 11, d 16 and 17, as well as from d 19 to d 21 of the experiment (treatment×day interaction; P<0.01). Cows receiving BST had greater (P≤0.01) mean glucose and NEFA concentrations, as well as reduced (P<0.01) mean RQUICKI during the experiment compared with control cohorts. No treatment effects, however, were detected (P=0.73) for serum P4 concentrations. In conclusion, results from this experiment indicate that hepatic P4 catabolism is not directly regulated by circulating IGF-I, whereas BST administration decreases insulin sensitivity in non-lactating dairy cows in adequate nutritional status. © 2013 Elsevier B.V. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 2014-05-27T11:29:35Z 2014-05-27T11:29:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2013.03.007 Livestock Science, v. 154, n. 1-3, p. 240-245, 2013. 1871-1413 http://hdl.handle.net/11449/75520 10.1016/j.livsci.2013.03.007 WOS:000319852200033 2-s2.0-84877107266 2-s2.0-84877107266.pdf 1069922096621313 |
url |
http://dx.doi.org/10.1016/j.livsci.2013.03.007 http://hdl.handle.net/11449/75520 |
identifier_str_mv |
Livestock Science, v. 154, n. 1-3, p. 240-245, 2013. 1871-1413 10.1016/j.livsci.2013.03.007 WOS:000319852200033 2-s2.0-84877107266 2-s2.0-84877107266.pdf 1069922096621313 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 1.204 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
240-245 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789378934734848 |