Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2019.11.025 http://hdl.handle.net/11449/198227 |
Resumo: | The objectives of this study were to determine if the utilization of a presynchronization strategy would improve fertility at first artificial insemination (AI) during an E2/P4 ovulation synchronization protocol with or without GnRH administration at the beginning of the protocol. This experiment was conducted using cows (n = 665) at their first postpartum service and the following breeding treatment: CIDR insertion and 2.0 mg of estradiol benzoate (EB) on day −11; 25 mg dinoprost tromethamine (PG) on day −4; PG, CIDR withdrawal, and 1.0 mg of estradiol cypionate (ECP) on day −2; timed-AI on day 0. At 31 ± 3 days postpartum, cows were randomly allocated to one of three treatments on a weekly basis: 1) -P + GnRH: cows assigned to the breeding protocol with 100 μg of GnRH on day −11, 2) P + GnRH: cows assigned to a presynchronization protocol using CIDR insertion +2 mg EB on day −28, PG + ECP and CIDR withdrawal on day −21, and beginning of the breeding protocol plus GnRH (100 μg) on day −11, and 3) +P-GnRH: cows assigned to a presynchronization protocol and the breeding treatment without GnRH on day −11. No treatment effects were observed on P/AI at the pregnancy diagnoses on days 32 and 60, or for pregnancy losses between days 32 and 60 of pregnancy whether analyses included all cows or only cows that ovulated near TAI. Moreover, milk yield negatively affected P/AI. Cows with greater circulating P4 concentrations on day −4 had greater P/AI on day 60. Cows without CL on day −11 had a reduced P/AI and this effect was more significant in cows not treated with GnRH. Cows assigned to -P + GnRH had the lowest circulating P4 concentration on day −4 (3.4 ± 0.16 ng/mL), followed by + P-GnRH (4.56 ± 0.17 ng/mL), and +P + GnRH (5.08 ± 0.17 ng/mL) cohorts. The data of the current study suggest that the combination of a Presynch and GnRH administration at the beginning of a TAI protocol was the most effective way to increase the % of cows with a functional CL and with elevated circulating P4 concentrations at the time of PG treatment. |
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Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cowsDairy cowsFertilityPresynchronizationProgesteroneTimed-AI protocolsThe objectives of this study were to determine if the utilization of a presynchronization strategy would improve fertility at first artificial insemination (AI) during an E2/P4 ovulation synchronization protocol with or without GnRH administration at the beginning of the protocol. This experiment was conducted using cows (n = 665) at their first postpartum service and the following breeding treatment: CIDR insertion and 2.0 mg of estradiol benzoate (EB) on day −11; 25 mg dinoprost tromethamine (PG) on day −4; PG, CIDR withdrawal, and 1.0 mg of estradiol cypionate (ECP) on day −2; timed-AI on day 0. At 31 ± 3 days postpartum, cows were randomly allocated to one of three treatments on a weekly basis: 1) -P + GnRH: cows assigned to the breeding protocol with 100 μg of GnRH on day −11, 2) P + GnRH: cows assigned to a presynchronization protocol using CIDR insertion +2 mg EB on day −28, PG + ECP and CIDR withdrawal on day −21, and beginning of the breeding protocol plus GnRH (100 μg) on day −11, and 3) +P-GnRH: cows assigned to a presynchronization protocol and the breeding treatment without GnRH on day −11. No treatment effects were observed on P/AI at the pregnancy diagnoses on days 32 and 60, or for pregnancy losses between days 32 and 60 of pregnancy whether analyses included all cows or only cows that ovulated near TAI. Moreover, milk yield negatively affected P/AI. Cows with greater circulating P4 concentrations on day −4 had greater P/AI on day 60. Cows without CL on day −11 had a reduced P/AI and this effect was more significant in cows not treated with GnRH. Cows assigned to -P + GnRH had the lowest circulating P4 concentration on day −4 (3.4 ± 0.16 ng/mL), followed by + P-GnRH (4.56 ± 0.17 ng/mL), and +P + GnRH (5.08 ± 0.17 ng/mL) cohorts. The data of the current study suggest that the combination of a Presynch and GnRH administration at the beginning of a TAI protocol was the most effective way to increase the % of cows with a functional CL and with elevated circulating P4 concentrations at the time of PG treatment.Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Department of Dairy Science University of WisconsinNutricorpDepartment of Animal Production São Paulo State UniversityFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Department of Animal Production São Paulo State UniversityUniversidade Estadual Paulista (Unesp)University of WisconsinNutricorpPereira, M. H.C. [UNESP]Wiltbank, M. C.Guida, T. G. [UNESP]Lopes, F. R. [UNESP]Cappellozza, B. I.Vasconcelos, J. L.M. [UNESP]2020-12-12T01:07:01Z2020-12-12T01:07:01Z2020-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article124-134http://dx.doi.org/10.1016/j.theriogenology.2019.11.025Theriogenology, v. 147, p. 124-134.0093-691Xhttp://hdl.handle.net/11449/19822710.1016/j.theriogenology.2019.11.0252-s2.0-85075904453Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2021-10-23T10:02:23Zoai:repositorio.unesp.br:11449/198227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:02:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
title |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
spellingShingle |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows Pereira, M. H.C. [UNESP] Dairy cows Fertility Presynchronization Progesterone Timed-AI protocols |
title_short |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
title_full |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
title_fullStr |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
title_full_unstemmed |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
title_sort |
Evaluation of presynchronization and addition of GnRH at the beginning of an estradiol/progesterone protocol on circulating progesterone and fertility of lactating dairy cows |
author |
Pereira, M. H.C. [UNESP] |
author_facet |
Pereira, M. H.C. [UNESP] Wiltbank, M. C. Guida, T. G. [UNESP] Lopes, F. R. [UNESP] Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
author_role |
author |
author2 |
Wiltbank, M. C. Guida, T. G. [UNESP] Lopes, F. R. [UNESP] Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Wisconsin Nutricorp |
dc.contributor.author.fl_str_mv |
Pereira, M. H.C. [UNESP] Wiltbank, M. C. Guida, T. G. [UNESP] Lopes, F. R. [UNESP] Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
dc.subject.por.fl_str_mv |
Dairy cows Fertility Presynchronization Progesterone Timed-AI protocols |
topic |
Dairy cows Fertility Presynchronization Progesterone Timed-AI protocols |
description |
The objectives of this study were to determine if the utilization of a presynchronization strategy would improve fertility at first artificial insemination (AI) during an E2/P4 ovulation synchronization protocol with or without GnRH administration at the beginning of the protocol. This experiment was conducted using cows (n = 665) at their first postpartum service and the following breeding treatment: CIDR insertion and 2.0 mg of estradiol benzoate (EB) on day −11; 25 mg dinoprost tromethamine (PG) on day −4; PG, CIDR withdrawal, and 1.0 mg of estradiol cypionate (ECP) on day −2; timed-AI on day 0. At 31 ± 3 days postpartum, cows were randomly allocated to one of three treatments on a weekly basis: 1) -P + GnRH: cows assigned to the breeding protocol with 100 μg of GnRH on day −11, 2) P + GnRH: cows assigned to a presynchronization protocol using CIDR insertion +2 mg EB on day −28, PG + ECP and CIDR withdrawal on day −21, and beginning of the breeding protocol plus GnRH (100 μg) on day −11, and 3) +P-GnRH: cows assigned to a presynchronization protocol and the breeding treatment without GnRH on day −11. No treatment effects were observed on P/AI at the pregnancy diagnoses on days 32 and 60, or for pregnancy losses between days 32 and 60 of pregnancy whether analyses included all cows or only cows that ovulated near TAI. Moreover, milk yield negatively affected P/AI. Cows with greater circulating P4 concentrations on day −4 had greater P/AI on day 60. Cows without CL on day −11 had a reduced P/AI and this effect was more significant in cows not treated with GnRH. Cows assigned to -P + GnRH had the lowest circulating P4 concentration on day −4 (3.4 ± 0.16 ng/mL), followed by + P-GnRH (4.56 ± 0.17 ng/mL), and +P + GnRH (5.08 ± 0.17 ng/mL) cohorts. The data of the current study suggest that the combination of a Presynch and GnRH administration at the beginning of a TAI protocol was the most effective way to increase the % of cows with a functional CL and with elevated circulating P4 concentrations at the time of PG treatment. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:07:01Z 2020-12-12T01:07:01Z 2020-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2019.11.025 Theriogenology, v. 147, p. 124-134. 0093-691X http://hdl.handle.net/11449/198227 10.1016/j.theriogenology.2019.11.025 2-s2.0-85075904453 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2019.11.025 http://hdl.handle.net/11449/198227 |
identifier_str_mv |
Theriogenology, v. 147, p. 124-134. 0093-691X 10.1016/j.theriogenology.2019.11.025 2-s2.0-85075904453 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
124-134 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789377982627840 |