Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10266-020-00495-y http://hdl.handle.net/11449/201566 |
Resumo: | Previous studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation. |
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Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogsAnimal modelLectinOral mucosaPalateWound healingPrevious studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Division of PeriodontologyDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University—UNESP, R Humaita, 1680Department of Implantology University of Santo AmaroDepartment of Dentistry II School of Dentistry Federal University of Maranhao—UFMADepartment of Cellular and Molecular Biology School of Medicine of Ribeirao Preto University of Sao PauloDepartment of Physiology and Pathology School of Dentistry Sao Paulo State University—UNESPDivision of PeriodontologyDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University—UNESP, R Humaita, 1680Department of Physiology and Pathology School of Dentistry Sao Paulo State University—UNESPFAPESP: 2006/60642-2FAPESP: 2009/16432-1FAPESP: 2015/21697-5Universidade Estadual Paulista (Unesp)University of Santo AmaroFederal University of Maranhao—UFMAUniversidade de São Paulo (USP)Kim, Yeon Jung [UNESP]de Molon, Rafael Scaf [UNESP]da Silva, Vanessa Camilada Veiga Conrado, Marina Cavalcanti AlbuquerqueSpolidório, Luis Carlos [UNESP]Roque-Barreira, Maria Cristina AntunesCirelli, Joni Augusto [UNESP]2020-12-12T02:35:59Z2020-12-12T02:35:59Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article560-568http://dx.doi.org/10.1007/s10266-020-00495-yOdontology, v. 108, n. 4, p. 560-568, 2020.1618-12551618-1247http://hdl.handle.net/11449/20156610.1007/s10266-020-00495-y2-s2.0-85079660323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOdontologyinfo:eu-repo/semantics/openAccess2024-09-27T14:05:06Zoai:repositorio.unesp.br:11449/201566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
title |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
spellingShingle |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs Kim, Yeon Jung [UNESP] Animal model Lectin Oral mucosa Palate Wound healing |
title_short |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
title_full |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
title_fullStr |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
title_full_unstemmed |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
title_sort |
Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs |
author |
Kim, Yeon Jung [UNESP] |
author_facet |
Kim, Yeon Jung [UNESP] de Molon, Rafael Scaf [UNESP] da Silva, Vanessa Camila da Veiga Conrado, Marina Cavalcanti Albuquerque Spolidório, Luis Carlos [UNESP] Roque-Barreira, Maria Cristina Antunes Cirelli, Joni Augusto [UNESP] |
author_role |
author |
author2 |
de Molon, Rafael Scaf [UNESP] da Silva, Vanessa Camila da Veiga Conrado, Marina Cavalcanti Albuquerque Spolidório, Luis Carlos [UNESP] Roque-Barreira, Maria Cristina Antunes Cirelli, Joni Augusto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Santo Amaro Federal University of Maranhao—UFMA Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Kim, Yeon Jung [UNESP] de Molon, Rafael Scaf [UNESP] da Silva, Vanessa Camila da Veiga Conrado, Marina Cavalcanti Albuquerque Spolidório, Luis Carlos [UNESP] Roque-Barreira, Maria Cristina Antunes Cirelli, Joni Augusto [UNESP] |
dc.subject.por.fl_str_mv |
Animal model Lectin Oral mucosa Palate Wound healing |
topic |
Animal model Lectin Oral mucosa Palate Wound healing |
description |
Previous studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:59Z 2020-12-12T02:35:59Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10266-020-00495-y Odontology, v. 108, n. 4, p. 560-568, 2020. 1618-1255 1618-1247 http://hdl.handle.net/11449/201566 10.1007/s10266-020-00495-y 2-s2.0-85079660323 |
url |
http://dx.doi.org/10.1007/s10266-020-00495-y http://hdl.handle.net/11449/201566 |
identifier_str_mv |
Odontology, v. 108, n. 4, p. 560-568, 2020. 1618-1255 1618-1247 10.1007/s10266-020-00495-y 2-s2.0-85079660323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Odontology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
560-568 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546431072436224 |