Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs

Detalhes bibliográficos
Autor(a) principal: Kim, Yeon Jung [UNESP]
Data de Publicação: 2020
Outros Autores: de Molon, Rafael Scaf [UNESP], da Silva, Vanessa Camila, da Veiga Conrado, Marina Cavalcanti Albuquerque, Spolidório, Luis Carlos [UNESP], Roque-Barreira, Maria Cristina Antunes, Cirelli, Joni Augusto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10266-020-00495-y
http://hdl.handle.net/11449/201566
Resumo: Previous studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation.
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spelling Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogsAnimal modelLectinOral mucosaPalateWound healingPrevious studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Division of PeriodontologyDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University—UNESP, R Humaita, 1680Department of Implantology University of Santo AmaroDepartment of Dentistry II School of Dentistry Federal University of Maranhao—UFMADepartment of Cellular and Molecular Biology School of Medicine of Ribeirao Preto University of Sao PauloDepartment of Physiology and Pathology School of Dentistry Sao Paulo State University—UNESPDivision of PeriodontologyDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University—UNESP, R Humaita, 1680Department of Physiology and Pathology School of Dentistry Sao Paulo State University—UNESPFAPESP: 2006/60642-2FAPESP: 2009/16432-1FAPESP: 2015/21697-5Universidade Estadual Paulista (Unesp)University of Santo AmaroFederal University of Maranhao—UFMAUniversidade de São Paulo (USP)Kim, Yeon Jung [UNESP]de Molon, Rafael Scaf [UNESP]da Silva, Vanessa Camilada Veiga Conrado, Marina Cavalcanti AlbuquerqueSpolidório, Luis Carlos [UNESP]Roque-Barreira, Maria Cristina AntunesCirelli, Joni Augusto [UNESP]2020-12-12T02:35:59Z2020-12-12T02:35:59Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article560-568http://dx.doi.org/10.1007/s10266-020-00495-yOdontology, v. 108, n. 4, p. 560-568, 2020.1618-12551618-1247http://hdl.handle.net/11449/20156610.1007/s10266-020-00495-y2-s2.0-85079660323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOdontologyinfo:eu-repo/semantics/openAccess2024-09-27T14:05:06Zoai:repositorio.unesp.br:11449/201566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
title Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
spellingShingle Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
Kim, Yeon Jung [UNESP]
Animal model
Lectin
Oral mucosa
Palate
Wound healing
title_short Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
title_full Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
title_fullStr Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
title_full_unstemmed Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
title_sort Topical application of lectin Artin M improves wound healing in defects created in the palatal mucosa: an in vivo study in dogs
author Kim, Yeon Jung [UNESP]
author_facet Kim, Yeon Jung [UNESP]
de Molon, Rafael Scaf [UNESP]
da Silva, Vanessa Camila
da Veiga Conrado, Marina Cavalcanti Albuquerque
Spolidório, Luis Carlos [UNESP]
Roque-Barreira, Maria Cristina Antunes
Cirelli, Joni Augusto [UNESP]
author_role author
author2 de Molon, Rafael Scaf [UNESP]
da Silva, Vanessa Camila
da Veiga Conrado, Marina Cavalcanti Albuquerque
Spolidório, Luis Carlos [UNESP]
Roque-Barreira, Maria Cristina Antunes
Cirelli, Joni Augusto [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Santo Amaro
Federal University of Maranhao—UFMA
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Kim, Yeon Jung [UNESP]
de Molon, Rafael Scaf [UNESP]
da Silva, Vanessa Camila
da Veiga Conrado, Marina Cavalcanti Albuquerque
Spolidório, Luis Carlos [UNESP]
Roque-Barreira, Maria Cristina Antunes
Cirelli, Joni Augusto [UNESP]
dc.subject.por.fl_str_mv Animal model
Lectin
Oral mucosa
Palate
Wound healing
topic Animal model
Lectin
Oral mucosa
Palate
Wound healing
description Previous studies have shown that topical application of lectin Artin-M accelerates wound healing in the rat oral mucosa. The aim of this study was to evaluate, by means of histology and immunohistochemistry (IHC) the effects of Artin-M on wound healing in the palatal mucosa in dogs. Three full thickness wounds of 6 mm diameter were surgically created in the palatal mucosa of twenty dogs and randomly divided into three groups according to one of the treatment assigned: Group C—Control (coagulum); Group A—Artin-M gel; Group V—Vehicle (carboxymethylcellulose 3%). Each animal received all the three experimental treatments. Afterwards, four animals were killed at 2, 4, 7, 14 and 21 days post-surgery. Wounded areas were photographed and scored for macroscopic evaluation. Biopsies were harvested and used for descriptive histological analysis, proliferating cell nuclear antigen IHC and measurement of myeloperoxidase activity. The results demonstrated faster wound closure in group A in comparison to the other groups in all the periods evaluated. Histological analyses exhibited improved re-epithelialization and collagen fiber formation resulting in faster maturation of granulation tissue in group A compared to the other groups by day 14. Treatment with Artin-M gel significantly induced cell proliferation and increased volumetric density of fibroblasts at day 2 and 4 (p < 0.05). Neutrophil infiltration in group A was significantly higher than the other groups (p < 0.05) at the same time points. Collectively, our findings demonstrated that Artin-M may potentially favor wound healing on palatal mucosa lesions via recruitment of neutrophils and promotion of cell proliferation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:35:59Z
2020-12-12T02:35:59Z
2020-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10266-020-00495-y
Odontology, v. 108, n. 4, p. 560-568, 2020.
1618-1255
1618-1247
http://hdl.handle.net/11449/201566
10.1007/s10266-020-00495-y
2-s2.0-85079660323
url http://dx.doi.org/10.1007/s10266-020-00495-y
http://hdl.handle.net/11449/201566
identifier_str_mv Odontology, v. 108, n. 4, p. 560-568, 2020.
1618-1255
1618-1247
10.1007/s10266-020-00495-y
2-s2.0-85079660323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Odontology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 560-568
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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