Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2021.105273 http://hdl.handle.net/11449/229702 |
Resumo: | Objectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm). Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm2 and 80 mW/cm2) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm2 shortly after LPS supplementation. The tests were performed 24 h after irradiation to assess mitochondrial activity (MTT assay), the number of viable cells (Trypan Blue), cell morphology (Scanning Electron Microscopy - SEM), and the quantification of Nitric Oxide (NO) and Reactive Oxygen Species (ROS). The data were analyzed using Kruskal–Wallis and Dunn's tests (p < 0.05). Results: The irradiated groups showed larger viable cells number than the non-irradiated group with LPS (p < 0.0001). All irradiation parameters decreased ROS concentrations after LPS application compared to the non-irradiated group (p < 0.05). All irradiation parameters enhanced the NO values compared to those of the control group (p < 0.05). The SEM images showed cells with regular morphology that adhered to the substrate. Conclusions: According to the parameters used in this study, the radiant exposure of 15 J/cm2 and irradiance of 40 mW/cm2 were the most effective irradiation parameters to stimulate and modulate oxidative stress in the primary teeth-derived dental pulp cells. |
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Repositório Institucional da UNESP |
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Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cellsDental pulp cellsInfrared LEDIrradiationReactive oxygen speciesObjectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm). Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm2 and 80 mW/cm2) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm2 shortly after LPS supplementation. The tests were performed 24 h after irradiation to assess mitochondrial activity (MTT assay), the number of viable cells (Trypan Blue), cell morphology (Scanning Electron Microscopy - SEM), and the quantification of Nitric Oxide (NO) and Reactive Oxygen Species (ROS). The data were analyzed using Kruskal–Wallis and Dunn's tests (p < 0.05). Results: The irradiated groups showed larger viable cells number than the non-irradiated group with LPS (p < 0.0001). All irradiation parameters decreased ROS concentrations after LPS application compared to the non-irradiated group (p < 0.05). All irradiation parameters enhanced the NO values compared to those of the control group (p < 0.05). The SEM images showed cells with regular morphology that adhered to the substrate. Conclusions: According to the parameters used in this study, the radiant exposure of 15 J/cm2 and irradiance of 40 mW/cm2 were the most effective irradiation parameters to stimulate and modulate oxidative stress in the primary teeth-derived dental pulp cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Pediatric Dentistry School of Dentistry Federal University of Uberlândia UFUDepartment of Endodontics School of Dentistry Federal University of Uberlandia UFUDepartment of Oral Pathology University of Ribeirão Preto UNAERPDepartment of Physiology and Pathology Araraquara School of Dentistry UNESPInstitute of Biomedical Sciences Federal University of Uberlandia UFUDepartment of Physiology and Pathology Araraquara School of Dentistry UNESPCNPq: 434204-2018-8Universidade Federal de Uberlândia (UFU)UNAERPUniversidade Estadual Paulista (UNESP)Bonvicini, Jéssica F.S.de Souza, Gabriela L.Basso, Fernanda G.de Souza Costa, Carlos A. [UNESP]Moura, Camilla C.G.Silva, Marcelo J.B.Turrioni, Ana Paula2022-04-29T08:35:24Z2022-04-29T08:35:24Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2021.105273Archives of Oral Biology, v. 131.1879-15060003-9969http://hdl.handle.net/11449/22970210.1016/j.archoralbio.2021.1052732-s2.0-85117086396Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:35:24Zoai:repositorio.unesp.br:11449/229702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:35:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
title |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
spellingShingle |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells Bonvicini, Jéssica F.S. Dental pulp cells Infrared LED Irradiation Reactive oxygen species |
title_short |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
title_full |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
title_fullStr |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
title_full_unstemmed |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
title_sort |
Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells |
author |
Bonvicini, Jéssica F.S. |
author_facet |
Bonvicini, Jéssica F.S. de Souza, Gabriela L. Basso, Fernanda G. de Souza Costa, Carlos A. [UNESP] Moura, Camilla C.G. Silva, Marcelo J.B. Turrioni, Ana Paula |
author_role |
author |
author2 |
de Souza, Gabriela L. Basso, Fernanda G. de Souza Costa, Carlos A. [UNESP] Moura, Camilla C.G. Silva, Marcelo J.B. Turrioni, Ana Paula |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) UNAERP Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Bonvicini, Jéssica F.S. de Souza, Gabriela L. Basso, Fernanda G. de Souza Costa, Carlos A. [UNESP] Moura, Camilla C.G. Silva, Marcelo J.B. Turrioni, Ana Paula |
dc.subject.por.fl_str_mv |
Dental pulp cells Infrared LED Irradiation Reactive oxygen species |
topic |
Dental pulp cells Infrared LED Irradiation Reactive oxygen species |
description |
Objectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm). Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm2 and 80 mW/cm2) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm2 shortly after LPS supplementation. The tests were performed 24 h after irradiation to assess mitochondrial activity (MTT assay), the number of viable cells (Trypan Blue), cell morphology (Scanning Electron Microscopy - SEM), and the quantification of Nitric Oxide (NO) and Reactive Oxygen Species (ROS). The data were analyzed using Kruskal–Wallis and Dunn's tests (p < 0.05). Results: The irradiated groups showed larger viable cells number than the non-irradiated group with LPS (p < 0.0001). All irradiation parameters decreased ROS concentrations after LPS application compared to the non-irradiated group (p < 0.05). All irradiation parameters enhanced the NO values compared to those of the control group (p < 0.05). The SEM images showed cells with regular morphology that adhered to the substrate. Conclusions: According to the parameters used in this study, the radiant exposure of 15 J/cm2 and irradiance of 40 mW/cm2 were the most effective irradiation parameters to stimulate and modulate oxidative stress in the primary teeth-derived dental pulp cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:35:24Z 2022-04-29T08:35:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2021.105273 Archives of Oral Biology, v. 131. 1879-1506 0003-9969 http://hdl.handle.net/11449/229702 10.1016/j.archoralbio.2021.105273 2-s2.0-85117086396 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2021.105273 http://hdl.handle.net/11449/229702 |
identifier_str_mv |
Archives of Oral Biology, v. 131. 1879-1506 0003-9969 10.1016/j.archoralbio.2021.105273 2-s2.0-85117086396 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045933292716032 |