Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2020v41n3p811 http://hdl.handle.net/11449/198710 |
Resumo: | Induction of resistance in common bean (Phaseolus vulgaris) has been considered a promising alternative to control anthracnose. Among the changes generated in the induction of resistance, structural changes have been reported by several authors as an efficient form of resistance to the stress plants undergo. Histochemical analysis techniques have been used to investigate tissue changes triggered by induction of resistance. Thus, this study aims to investigate certain histochemical changes suffered by common bean plants induced with potassium phosphite and Trichoderma spp. in response to the attack by Colletotrichum lindemuthianum, aiming to determine the host response pattern in terms of structural changes, associating it to possible disease control. Treatments consisted of isolates T. harzianum (isolate TOD1) and T. virens (isolate TM4), leaf fertilizer potassium phosphite Fertilis®, and distilled water (control). Inducers were applied to common bean alone or associated, consisting of five treatments plus the control treatment. The six treatments were evaluated for the absence and presence of C. lindemuthianum in a factorial scheme (6×2). Treatments allowed evaluating the severity of anthracnose in common bean, location of H2O2, lignin deposition, and hypersensitivity response in common bean hypocotyl by histochemical staining. Potassium phosphite and combinations of T. virens and T. harzianum with potassium phosphite efficiently reduced disease severity under greenhouse conditions, reaching 68, 84, and 71%, respectively. Studies with hypocotyl showed that T. harzianum + potassium phosphite and T. virens + potassium phosphite accelerated the H2O2 accumulation process and lignin deposition at the pathogen penetration site, in addition to the hypersensitivity reaction through the resistance-inducing activity, contributing to the protection of common bean against anthracnose caused by C. lindemuthianum. |
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Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianumAnthracnoseHypersensitivity responseInduction of resistanceOxygen-reactive speciesInduction of resistance in common bean (Phaseolus vulgaris) has been considered a promising alternative to control anthracnose. Among the changes generated in the induction of resistance, structural changes have been reported by several authors as an efficient form of resistance to the stress plants undergo. Histochemical analysis techniques have been used to investigate tissue changes triggered by induction of resistance. Thus, this study aims to investigate certain histochemical changes suffered by common bean plants induced with potassium phosphite and Trichoderma spp. in response to the attack by Colletotrichum lindemuthianum, aiming to determine the host response pattern in terms of structural changes, associating it to possible disease control. Treatments consisted of isolates T. harzianum (isolate TOD1) and T. virens (isolate TM4), leaf fertilizer potassium phosphite Fertilis®, and distilled water (control). Inducers were applied to common bean alone or associated, consisting of five treatments plus the control treatment. The six treatments were evaluated for the absence and presence of C. lindemuthianum in a factorial scheme (6×2). Treatments allowed evaluating the severity of anthracnose in common bean, location of H2O2, lignin deposition, and hypersensitivity response in common bean hypocotyl by histochemical staining. Potassium phosphite and combinations of T. virens and T. harzianum with potassium phosphite efficiently reduced disease severity under greenhouse conditions, reaching 68, 84, and 71%, respectively. Studies with hypocotyl showed that T. harzianum + potassium phosphite and T. virens + potassium phosphite accelerated the H2O2 accumulation process and lignin deposition at the pathogen penetration site, in addition to the hypersensitivity reaction through the resistance-inducing activity, contributing to the protection of common bean against anthracnose caused by C. lindemuthianum.Instituto Federal do Paraná IFPRUniversidade Estadual do Oeste do Paraná UNIOESTEUniversidade Estadual Paulista Júlio de Mesquita Filho UNESPIFPRUniversidade Estadual Paulista Júlio de Mesquita Filho UNESPIFPRUNIOESTEUniversidade Estadual Paulista (Unesp)Figueira, Eliana Peliçon PereiraKuhn, Odair JoséMartinazzo-Portz, TatianeStangarlin, José RenatoPereira, Marcos Donizete Peliçon [UNESP]Lampugnani, Camila2020-12-12T01:20:05Z2020-12-12T01:20:05Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article811-828http://dx.doi.org/10.5433/1679-0359.2020v41n3p811Semina:Ciencias Agrarias, v. 41, n. 3, p. 811-828, 2020.1679-03591676-546Xhttp://hdl.handle.net/11449/19871010.5433/1679-0359.2020v41n3p8112-s2.0-85082922516Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2021-10-22T19:50:45Zoai:repositorio.unesp.br:11449/198710Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T19:50:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
title |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
spellingShingle |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum Figueira, Eliana Peliçon Pereira Anthracnose Hypersensitivity response Induction of resistance Oxygen-reactive species |
title_short |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
title_full |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
title_fullStr |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
title_full_unstemmed |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
title_sort |
Histochemical changes induced by Trichoderma spp. And potassium phosphite in common bean (Phaseolus vulgaris) in response to the attack by Colletotrichum lindemuthianum |
author |
Figueira, Eliana Peliçon Pereira |
author_facet |
Figueira, Eliana Peliçon Pereira Kuhn, Odair José Martinazzo-Portz, Tatiane Stangarlin, José Renato Pereira, Marcos Donizete Peliçon [UNESP] Lampugnani, Camila |
author_role |
author |
author2 |
Kuhn, Odair José Martinazzo-Portz, Tatiane Stangarlin, José Renato Pereira, Marcos Donizete Peliçon [UNESP] Lampugnani, Camila |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
IFPR UNIOESTE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Figueira, Eliana Peliçon Pereira Kuhn, Odair José Martinazzo-Portz, Tatiane Stangarlin, José Renato Pereira, Marcos Donizete Peliçon [UNESP] Lampugnani, Camila |
dc.subject.por.fl_str_mv |
Anthracnose Hypersensitivity response Induction of resistance Oxygen-reactive species |
topic |
Anthracnose Hypersensitivity response Induction of resistance Oxygen-reactive species |
description |
Induction of resistance in common bean (Phaseolus vulgaris) has been considered a promising alternative to control anthracnose. Among the changes generated in the induction of resistance, structural changes have been reported by several authors as an efficient form of resistance to the stress plants undergo. Histochemical analysis techniques have been used to investigate tissue changes triggered by induction of resistance. Thus, this study aims to investigate certain histochemical changes suffered by common bean plants induced with potassium phosphite and Trichoderma spp. in response to the attack by Colletotrichum lindemuthianum, aiming to determine the host response pattern in terms of structural changes, associating it to possible disease control. Treatments consisted of isolates T. harzianum (isolate TOD1) and T. virens (isolate TM4), leaf fertilizer potassium phosphite Fertilis®, and distilled water (control). Inducers were applied to common bean alone or associated, consisting of five treatments plus the control treatment. The six treatments were evaluated for the absence and presence of C. lindemuthianum in a factorial scheme (6×2). Treatments allowed evaluating the severity of anthracnose in common bean, location of H2O2, lignin deposition, and hypersensitivity response in common bean hypocotyl by histochemical staining. Potassium phosphite and combinations of T. virens and T. harzianum with potassium phosphite efficiently reduced disease severity under greenhouse conditions, reaching 68, 84, and 71%, respectively. Studies with hypocotyl showed that T. harzianum + potassium phosphite and T. virens + potassium phosphite accelerated the H2O2 accumulation process and lignin deposition at the pathogen penetration site, in addition to the hypersensitivity reaction through the resistance-inducing activity, contributing to the protection of common bean against anthracnose caused by C. lindemuthianum. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:20:05Z 2020-12-12T01:20:05Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2020v41n3p811 Semina:Ciencias Agrarias, v. 41, n. 3, p. 811-828, 2020. 1679-0359 1676-546X http://hdl.handle.net/11449/198710 10.5433/1679-0359.2020v41n3p811 2-s2.0-85082922516 |
url |
http://dx.doi.org/10.5433/1679-0359.2020v41n3p811 http://hdl.handle.net/11449/198710 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 41, n. 3, p. 811-828, 2020. 1679-0359 1676-546X 10.5433/1679-0359.2020v41n3p811 2-s2.0-85082922516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina:Ciencias Agrarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
811-828 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803649600953778176 |