Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2013000400007 http://hdl.handle.net/11449/227498 |
Resumo: | Microsatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, HE = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur. |
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Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive SuccessCoturnix JaponicaParentage testingPolyan-dryQuailSSRsMicrosatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, HE = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur.Department of Genetics Biosciences Institute São Paulo State University (UNESP), Botucatu, SPDepartment of Genetics and Evolution Federal University of São Carlos (UFSCar), São Carlos, SPDepartment of Genetics Biosciences Institute São Paulo State University (UNESP), Botucatu, SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Gomes, G. L. [UNESP]Hatanaka, T.de Campos, W. N.Wasko, A. P. [UNESP]2022-04-29T07:13:30Z2022-04-29T07:13:30Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article329-338http://dx.doi.org/10.1590/S1516-635X2013000400007Revista Brasileira de Ciencia Avicola, v. 15, n. 4, p. 329-338, 2013.1516-635Xhttp://hdl.handle.net/11449/22749810.1590/S1516-635X20130004000072-s2.0-84892863522Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciencia Avicolainfo:eu-repo/semantics/openAccess2022-04-29T07:13:30Zoai:repositorio.unesp.br:11449/227498Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:13:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
title |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
spellingShingle |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success Gomes, G. L. [UNESP] Coturnix Japonica Parentage testing Polyan-dry Quail SSRs |
title_short |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
title_full |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
title_fullStr |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
title_full_unstemmed |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
title_sort |
Assessing paternity in Japanese quails (Coturnix Japonica) using microsatellite Markers - Inferences for its mating system and reproductive Success |
author |
Gomes, G. L. [UNESP] |
author_facet |
Gomes, G. L. [UNESP] Hatanaka, T. de Campos, W. N. Wasko, A. P. [UNESP] |
author_role |
author |
author2 |
Hatanaka, T. de Campos, W. N. Wasko, A. P. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Gomes, G. L. [UNESP] Hatanaka, T. de Campos, W. N. Wasko, A. P. [UNESP] |
dc.subject.por.fl_str_mv |
Coturnix Japonica Parentage testing Polyan-dry Quail SSRs |
topic |
Coturnix Japonica Parentage testing Polyan-dry Quail SSRs |
description |
Microsatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, HE = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 2022-04-29T07:13:30Z 2022-04-29T07:13:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2013000400007 Revista Brasileira de Ciencia Avicola, v. 15, n. 4, p. 329-338, 2013. 1516-635X http://hdl.handle.net/11449/227498 10.1590/S1516-635X2013000400007 2-s2.0-84892863522 |
url |
http://dx.doi.org/10.1590/S1516-635X2013000400007 http://hdl.handle.net/11449/227498 |
identifier_str_mv |
Revista Brasileira de Ciencia Avicola, v. 15, n. 4, p. 329-338, 2013. 1516-635X 10.1590/S1516-635X2013000400007 2-s2.0-84892863522 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciencia Avicola |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
329-338 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046067181191168 |