Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2013000400007 http://hdl.handle.net/11449/112627 |
Resumo: | Microsatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, H-E = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur. |
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Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive SuccessCoturnix japonicaparentage testingpolyandryquailSSRsMicrosatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, H-E = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, BrazilFed Univ Sao Carlos UFSCar, Dept Genet & Evolut, Sao Carlos, SP, BrazilUniv Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, BrazilFacta-fundacio Arnco Ciência Tecnologia AvicolasUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Gomes, M. L. [UNESP]Hatanaka, T.Campos, W. N. deWasko, A. P. [UNESP]2014-12-03T13:10:53Z2014-12-03T13:10:53Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article329-338application/pdfhttp://dx.doi.org/10.1590/S1516-635X2013000400007Brazilian Journal of Poultry Science. Campinas: Facta-fundacio Arnco Ciencia Tecnologia Avicolas, v. 15, n. 4, p. 329-338, 2013.1516-635Xhttp://hdl.handle.net/11449/112627S1516-635X2013000400007WOS:000330032300007S1516-635X2013000400007.pdf19268639173789090000-0003-4600-0367Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Poultry Science0.463info:eu-repo/semantics/openAccess2023-10-01T06:01:27Zoai:repositorio.unesp.br:11449/112627Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T11:06:45.470471Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
title |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
spellingShingle |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success Gomes, M. L. [UNESP] Coturnix japonica parentage testing polyandry quail SSRs |
title_short |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
title_full |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
title_fullStr |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
title_full_unstemmed |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
title_sort |
Assessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Success |
author |
Gomes, M. L. [UNESP] |
author_facet |
Gomes, M. L. [UNESP] Hatanaka, T. Campos, W. N. de Wasko, A. P. [UNESP] |
author_role |
author |
author2 |
Hatanaka, T. Campos, W. N. de Wasko, A. P. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Gomes, M. L. [UNESP] Hatanaka, T. Campos, W. N. de Wasko, A. P. [UNESP] |
dc.subject.por.fl_str_mv |
Coturnix japonica parentage testing polyandry quail SSRs |
topic |
Coturnix japonica parentage testing polyandry quail SSRs |
description |
Microsatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, H-E = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 2014-12-03T13:10:53Z 2014-12-03T13:10:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2013000400007 Brazilian Journal of Poultry Science. Campinas: Facta-fundacio Arnco Ciencia Tecnologia Avicolas, v. 15, n. 4, p. 329-338, 2013. 1516-635X http://hdl.handle.net/11449/112627 S1516-635X2013000400007 WOS:000330032300007 S1516-635X2013000400007.pdf 1926863917378909 0000-0003-4600-0367 |
url |
http://dx.doi.org/10.1590/S1516-635X2013000400007 http://hdl.handle.net/11449/112627 |
identifier_str_mv |
Brazilian Journal of Poultry Science. Campinas: Facta-fundacio Arnco Ciencia Tecnologia Avicolas, v. 15, n. 4, p. 329-338, 2013. 1516-635X S1516-635X2013000400007 WOS:000330032300007 S1516-635X2013000400007.pdf 1926863917378909 0000-0003-4600-0367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Poultry Science 0.463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
329-338 application/pdf |
dc.publisher.none.fl_str_mv |
Facta-fundacio Arnco Ciência Tecnologia Avicolas |
publisher.none.fl_str_mv |
Facta-fundacio Arnco Ciência Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045771594956800 |