Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media

Detalhes bibliográficos
Autor(a) principal: Travensolo,Regiane de Fátima
Data de Publicação: 2009
Outros Autores: Costa,Maria Vitória Cecchette Gottardi, Carareto-Alves,Lucia Maria, Carrilho,Emanuel, Lemos,Eliana Gertrudes de Macedo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300006
Resumo: DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.
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spelling Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture mediaDNA MicroarrayXylella fastidiosaexpressiontranscriptomeDNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.Instituto de Tecnologia do Paraná - Tecpar2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300006Brazilian Archives of Biology and Technology v.52 n.3 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000300006info:eu-repo/semantics/openAccessTravensolo,Regiane de FátimaCosta,Maria Vitória Cecchette GottardiCarareto-Alves,Lucia MariaCarrilho,EmanuelLemos,Eliana Gertrudes de Macedoeng2009-07-22T00:00:00Zoai:scielo:S1516-89132009000300006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-07-22T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
title Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
spellingShingle Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
Travensolo,Regiane de Fátima
DNA Microarray
Xylella fastidiosa
expression
transcriptome
title_short Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
title_full Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
title_fullStr Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
title_full_unstemmed Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
title_sort Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
author Travensolo,Regiane de Fátima
author_facet Travensolo,Regiane de Fátima
Costa,Maria Vitória Cecchette Gottardi
Carareto-Alves,Lucia Maria
Carrilho,Emanuel
Lemos,Eliana Gertrudes de Macedo
author_role author
author2 Costa,Maria Vitória Cecchette Gottardi
Carareto-Alves,Lucia Maria
Carrilho,Emanuel
Lemos,Eliana Gertrudes de Macedo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Travensolo,Regiane de Fátima
Costa,Maria Vitória Cecchette Gottardi
Carareto-Alves,Lucia Maria
Carrilho,Emanuel
Lemos,Eliana Gertrudes de Macedo
dc.subject.por.fl_str_mv DNA Microarray
Xylella fastidiosa
expression
transcriptome
topic DNA Microarray
Xylella fastidiosa
expression
transcriptome
description DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.
publishDate 2009
dc.date.none.fl_str_mv 2009-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000300006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.3 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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