Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

Detalhes bibliográficos
Autor(a) principal: Christofoletti, Cintya A. [UNESP]
Data de Publicação: 2009
Outros Autores: David, José Augusto O., Fontanetti, Carmem S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572009005000020
http://hdl.handle.net/11449/19640
Resumo: The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
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spelling Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparisoncomet assayethidium bromidesilver stainingtilapiaThe present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de BiologiaUniversidade Federal do Pará (UFPA) Instituto de Biociências Departamento de GenéticaUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de BiologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade Federal do Pará (UFPA)Christofoletti, Cintya A. [UNESP]David, José Augusto O.Fontanetti, Carmem S. [UNESP]2014-05-20T13:54:50Z2014-05-20T13:54:50Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article155-158application/pdfhttp://dx.doi.org/10.1590/S1415-47572009005000020Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 32, n. 1, p. 155-158, 2009.1415-4757http://hdl.handle.net/11449/1964010.1590/S1415-47572009005000020S1415-47572009000100024WOS:000263377700024S1415-47572009000100024.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-02T06:15:55Zoai:repositorio.unesp.br:11449/19640Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:19:36.688352Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
title Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
spellingShingle Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Christofoletti, Cintya A. [UNESP]
comet assay
ethidium bromide
silver staining
tilapia
title_short Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
title_full Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
title_fullStr Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
title_full_unstemmed Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
title_sort Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
author Christofoletti, Cintya A. [UNESP]
author_facet Christofoletti, Cintya A. [UNESP]
David, José Augusto O.
Fontanetti, Carmem S. [UNESP]
author_role author
author2 David, José Augusto O.
Fontanetti, Carmem S. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Pará (UFPA)
dc.contributor.author.fl_str_mv Christofoletti, Cintya A. [UNESP]
David, José Augusto O.
Fontanetti, Carmem S. [UNESP]
dc.subject.por.fl_str_mv comet assay
ethidium bromide
silver staining
tilapia
topic comet assay
ethidium bromide
silver staining
tilapia
description The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2014-05-20T13:54:50Z
2014-05-20T13:54:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572009005000020
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 32, n. 1, p. 155-158, 2009.
1415-4757
http://hdl.handle.net/11449/19640
10.1590/S1415-47572009005000020
S1415-47572009000100024
WOS:000263377700024
S1415-47572009000100024.pdf
url http://dx.doi.org/10.1590/S1415-47572009005000020
http://hdl.handle.net/11449/19640
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 32, n. 1, p. 155-158, 2009.
1415-4757
10.1590/S1415-47572009005000020
S1415-47572009000100024
WOS:000263377700024
S1415-47572009000100024.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 155-158
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129052316270592

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