Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/6875 |
Resumo: | The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination. |
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2015-09-10T15:06:59Z2015-09-10T15:06:59Z2009CHRISTOFOLETTI, Cintya Aparecida; DAVID, José Augusto Oliveira; FONTANETTI, Carmem Silvia. Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison. Genetics and Molecular Biology, São Paulo, v. 32, n. 1, p. 155-158, 2009. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024&lng=pt&nrm=iso>. Acesso em: 8 set. 2015. <http://dx.doi.org/10.1590/S1415-47572009005000020>.1678-4685http://repositorio.ufpa.br/jspui/handle/2011/6875The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.engPeixeTilápia-do-NiloOreochromis niloticusDNAEnsaio cometaImpregnação pela prataEtídioApplication of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparisoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCHRISTOFOLETTI, Cintya AparecidaDAVID, José Augusto de OliveiraFONTANETTI, Carmem Silviainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_ApplicationCometAssay.pdfArtigo_ApplicationCometAssay.pdfapplication/pdf81043http://repositorio.ufpa.br/oai/bitstream/2011/6875/1/Artigo_ApplicationCometAssay.pdf04d9c31a7cba9d5aa733af9fb52b9ad1MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6875/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821328http://repositorio.ufpa.br/oai/bitstream/2011/6875/3/license_text1ddceb2a2635a9472b3c10d731d0906eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6875/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/6875/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_ApplicationCometAssay.pdf.txtArtigo_ApplicationCometAssay.pdf.txtExtracted texttext/plain21127http://repositorio.ufpa.br/oai/bitstream/2011/6875/6/Artigo_ApplicationCometAssay.pdf.txt98afd83ff92a8a77363ba5b1fb75f38bMD562011/68752017-12-14 10:26:26.211oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-12-14T13:26:26Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
title |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
spellingShingle |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison CHRISTOFOLETTI, Cintya Aparecida Peixe Tilápia-do-Nilo Oreochromis niloticus DNA Ensaio cometa Impregnação pela prata Etídio |
title_short |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
title_full |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
title_fullStr |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
title_full_unstemmed |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
title_sort |
Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison |
author |
CHRISTOFOLETTI, Cintya Aparecida |
author_facet |
CHRISTOFOLETTI, Cintya Aparecida DAVID, José Augusto de Oliveira FONTANETTI, Carmem Silvia |
author_role |
author |
author2 |
DAVID, José Augusto de Oliveira FONTANETTI, Carmem Silvia |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
CHRISTOFOLETTI, Cintya Aparecida DAVID, José Augusto de Oliveira FONTANETTI, Carmem Silvia |
dc.subject.por.fl_str_mv |
Peixe Tilápia-do-Nilo Oreochromis niloticus DNA Ensaio cometa Impregnação pela prata Etídio |
topic |
Peixe Tilápia-do-Nilo Oreochromis niloticus DNA Ensaio cometa Impregnação pela prata Etídio |
description |
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2015-09-10T15:06:59Z |
dc.date.available.fl_str_mv |
2015-09-10T15:06:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CHRISTOFOLETTI, Cintya Aparecida; DAVID, José Augusto Oliveira; FONTANETTI, Carmem Silvia. Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison. Genetics and Molecular Biology, São Paulo, v. 32, n. 1, p. 155-158, 2009. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024&lng=pt&nrm=iso>. Acesso em: 8 set. 2015. <http://dx.doi.org/10.1590/S1415-47572009005000020>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/6875 |
dc.identifier.issn.none.fl_str_mv |
1678-4685 |
identifier_str_mv |
CHRISTOFOLETTI, Cintya Aparecida; DAVID, José Augusto Oliveira; FONTANETTI, Carmem Silvia. Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison. Genetics and Molecular Biology, São Paulo, v. 32, n. 1, p. 155-158, 2009. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024&lng=pt&nrm=iso>. Acesso em: 8 set. 2015. <http://dx.doi.org/10.1590/S1415-47572009005000020>. 1678-4685 |
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http://repositorio.ufpa.br/jspui/handle/2011/6875 |
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eng |
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