Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin

Detalhes bibliográficos
Autor(a) principal: Cavalcante Esposito, Ana Claudia [UNESP]
Data de Publicação: 2020
Outros Autores: Brianezi, Gabrielli [UNESP], Souza, Nathalia Pereira de [UNESP], Bartoli Miot, Luciane Donida [UNESP], Miot, Helio Amante [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5021/ad.2020.32.2.101
http://hdl.handle.net/11449/196696
Resumo: Background: Melasma is a chronic acquired focal hyper-melanosis which pathogenesis has not been fully elucidated. Classical pathophysiologic studies have analysed the affected and perilesional areas, but little is known about the status of sun-protected skin, which is subjected to the same endogenous and genetic factors. Objective: To assess the histological characteristics of melasma compared to adjacent and retroauricular skin. Methods: Skin samples were collected from 10 female from: melasma, perilesional area and retroauricular. The samples were stained (haematoxyl in-eosin, periodic acid-Schiff, Fontana-Masson, picrosiri us red, toluidine blue and Verhoeff), immunolabelled for CD34 and Wnt1. The data from the skin sites were analysed simultaneously by a multivariate model. Results: Melasma skin exhibited noteworthy stratum corneum compaction, greater collagen heterogeneity, solar elastosis, higher number of mast cells, basement membrane zone (BMZ) damage, Wnt1 expression, pendulum melanocytes, higher cell ularity and vascular proliferation at the superficial dermis. Stratum corneum compaction, collagen heterogeneity and BMZ abnormalities were variables associated to melasma that not follow a continuum through retroauricular to adjacent skin. Mast cell count was the variable that disclosed correlation with the most other abnormalities as well as had the greater contribution in the multivariate model. Conclusion: In addition to melanocyte hyperactivity, melasma skin exhibits alterations in the epidermal barrier, upper dermis and BMZ, which differ from the adjacent sun-exposed skin and retroauricular skin, indicating a distinct phenotype, rather than a mere extension of photoageing or intrinsic ageing. Mast cells appear to play a central role in the physiopathology of melasma.
id UNSP_0323602fd79b0cdf2c7d255949f72c82
oai_identifier_str oai:repositorio.unesp.br:11449/196696
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular SkinBasement membraneDermisEpidermisMelanosisBackground: Melasma is a chronic acquired focal hyper-melanosis which pathogenesis has not been fully elucidated. Classical pathophysiologic studies have analysed the affected and perilesional areas, but little is known about the status of sun-protected skin, which is subjected to the same endogenous and genetic factors. Objective: To assess the histological characteristics of melasma compared to adjacent and retroauricular skin. Methods: Skin samples were collected from 10 female from: melasma, perilesional area and retroauricular. The samples were stained (haematoxyl in-eosin, periodic acid-Schiff, Fontana-Masson, picrosiri us red, toluidine blue and Verhoeff), immunolabelled for CD34 and Wnt1. The data from the skin sites were analysed simultaneously by a multivariate model. Results: Melasma skin exhibited noteworthy stratum corneum compaction, greater collagen heterogeneity, solar elastosis, higher number of mast cells, basement membrane zone (BMZ) damage, Wnt1 expression, pendulum melanocytes, higher cell ularity and vascular proliferation at the superficial dermis. Stratum corneum compaction, collagen heterogeneity and BMZ abnormalities were variables associated to melasma that not follow a continuum through retroauricular to adjacent skin. Mast cell count was the variable that disclosed correlation with the most other abnormalities as well as had the greater contribution in the multivariate model. Conclusion: In addition to melanocyte hyperactivity, melasma skin exhibits alterations in the epidermal barrier, upper dermis and BMZ, which differ from the adjacent sun-exposed skin and retroauricular skin, indicating a distinct phenotype, rather than a mere extension of photoageing or intrinsic ageing. Mast cells appear to play a central role in the physiopathology of melasma.FUNADERSPSao Paulo State Univ, Botucatu Sch Med, Dept Dermatol & Radioterapy, BR-18618000 Botucatu, SP, BrazilSao Paulo State Univ, Botucatu Sch Med, Dept Patol, Botucatu, SP, BrazilSao Paulo State Univ, Botucatu Sch Med, Dept Dermatol & Radioterapy, BR-18618000 Botucatu, SP, BrazilSao Paulo State Univ, Botucatu Sch Med, Dept Patol, Botucatu, SP, Brazil: CNPq -401309/2016-9Korean Dermatological AssocUniversidade Estadual Paulista (Unesp)Cavalcante Esposito, Ana Claudia [UNESP]Brianezi, Gabrielli [UNESP]Souza, Nathalia Pereira de [UNESP]Bartoli Miot, Luciane Donida [UNESP]Miot, Helio Amante [UNESP]2020-12-10T19:53:16Z2020-12-10T19:53:16Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-108http://dx.doi.org/10.5021/ad.2020.32.2.101Annals Of Dermatology. Seoul: Korean Dermatological Assoc, v. 32, n. 2, p. 101-108, 2020.1013-9087http://hdl.handle.net/11449/19669610.5021/ad.2020.32.2.101WOS:000520021900002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnnals Of Dermatologyinfo:eu-repo/semantics/openAccess2024-09-03T13:15:04Zoai:repositorio.unesp.br:11449/196696Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
title Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
spellingShingle Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
Cavalcante Esposito, Ana Claudia [UNESP]
Basement membrane
Dermis
Epidermis
Melanosis
title_short Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
title_full Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
title_fullStr Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
title_full_unstemmed Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
title_sort Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin
author Cavalcante Esposito, Ana Claudia [UNESP]
author_facet Cavalcante Esposito, Ana Claudia [UNESP]
Brianezi, Gabrielli [UNESP]
Souza, Nathalia Pereira de [UNESP]
Bartoli Miot, Luciane Donida [UNESP]
Miot, Helio Amante [UNESP]
author_role author
author2 Brianezi, Gabrielli [UNESP]
Souza, Nathalia Pereira de [UNESP]
Bartoli Miot, Luciane Donida [UNESP]
Miot, Helio Amante [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cavalcante Esposito, Ana Claudia [UNESP]
Brianezi, Gabrielli [UNESP]
Souza, Nathalia Pereira de [UNESP]
Bartoli Miot, Luciane Donida [UNESP]
Miot, Helio Amante [UNESP]
dc.subject.por.fl_str_mv Basement membrane
Dermis
Epidermis
Melanosis
topic Basement membrane
Dermis
Epidermis
Melanosis
description Background: Melasma is a chronic acquired focal hyper-melanosis which pathogenesis has not been fully elucidated. Classical pathophysiologic studies have analysed the affected and perilesional areas, but little is known about the status of sun-protected skin, which is subjected to the same endogenous and genetic factors. Objective: To assess the histological characteristics of melasma compared to adjacent and retroauricular skin. Methods: Skin samples were collected from 10 female from: melasma, perilesional area and retroauricular. The samples were stained (haematoxyl in-eosin, periodic acid-Schiff, Fontana-Masson, picrosiri us red, toluidine blue and Verhoeff), immunolabelled for CD34 and Wnt1. The data from the skin sites were analysed simultaneously by a multivariate model. Results: Melasma skin exhibited noteworthy stratum corneum compaction, greater collagen heterogeneity, solar elastosis, higher number of mast cells, basement membrane zone (BMZ) damage, Wnt1 expression, pendulum melanocytes, higher cell ularity and vascular proliferation at the superficial dermis. Stratum corneum compaction, collagen heterogeneity and BMZ abnormalities were variables associated to melasma that not follow a continuum through retroauricular to adjacent skin. Mast cell count was the variable that disclosed correlation with the most other abnormalities as well as had the greater contribution in the multivariate model. Conclusion: In addition to melanocyte hyperactivity, melasma skin exhibits alterations in the epidermal barrier, upper dermis and BMZ, which differ from the adjacent sun-exposed skin and retroauricular skin, indicating a distinct phenotype, rather than a mere extension of photoageing or intrinsic ageing. Mast cells appear to play a central role in the physiopathology of melasma.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T19:53:16Z
2020-12-10T19:53:16Z
2020-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5021/ad.2020.32.2.101
Annals Of Dermatology. Seoul: Korean Dermatological Assoc, v. 32, n. 2, p. 101-108, 2020.
1013-9087
http://hdl.handle.net/11449/196696
10.5021/ad.2020.32.2.101
WOS:000520021900002
url http://dx.doi.org/10.5021/ad.2020.32.2.101
http://hdl.handle.net/11449/196696
identifier_str_mv Annals Of Dermatology. Seoul: Korean Dermatological Assoc, v. 32, n. 2, p. 101-108, 2020.
1013-9087
10.5021/ad.2020.32.2.101
WOS:000520021900002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Annals Of Dermatology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 101-108
dc.publisher.none.fl_str_mv Korean Dermatological Assoc
publisher.none.fl_str_mv Korean Dermatological Assoc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021378105016320