Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep

Detalhes bibliográficos
Autor(a) principal: Zafalon, Luiz F.
Data de Publicação: 2018
Outros Autores: Cunha, Maria L.R.S. [UNESP], Brandão, Humberto M., Mosqueira, Vanessa C.F., Santana, Raul C.M., Júnior, Waldomiro Barioni, Martins, Katheryne B. [UNESP], Pilon, Lucas E. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-5150-PVB-4984
http://hdl.handle.net/11449/171173
Resumo: Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.
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spelling Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheepAntimicrobial treatmentBacteriosesBiofilmsCoagulase-negativeEnterotoxinsMastitisSheepStaphylococcus spp.Virulence factor genesCoagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Embrapa Pecuária Sudeste Rodovia Washington Luiz, Km 234, Cx. Postal 339Instituto de Biociências Universidade Estadual Paulista (Unesp), Distrito de Rubião JúniorEmbrapa Gado de Leite, Rua Eugênio do Nascimento 610Universidade Federal de Ouro Preto, Rua Costa Sena 171Programa de Pós-Graduação em Medicina Veterinária Preventiva Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato CastellaneInstituto de Biociências Universidade Estadual Paulista (Unesp), Distrito de Rubião JúniorPrograma de Pós-Graduação em Medicina Veterinária Preventiva Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato CastellaneFAPESP: 2012/23044-0Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade Federal de Ouro PretoZafalon, Luiz F.Cunha, Maria L.R.S. [UNESP]Brandão, Humberto M.Mosqueira, Vanessa C.F.Santana, Raul C.M.Júnior, Waldomiro BarioniMartins, Katheryne B. [UNESP]Pilon, Lucas E. [UNESP]2018-12-11T16:54:14Z2018-12-11T16:54:14Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article579-585application/pdfhttp://dx.doi.org/10.1590/1678-5150-PVB-4984Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018.1678-51500100-736Xhttp://hdl.handle.net/11449/17117310.1590/1678-5150-PVB-4984S0100-736X20180004005792-s2.0-85049455998S0100-736X2018000400579.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-11-07T06:12:04Zoai:repositorio.unesp.br:11449/171173Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:05:11.698988Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
title Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
spellingShingle Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
Zafalon, Luiz F.
Antimicrobial treatment
Bacterioses
Biofilms
Coagulase-negative
Enterotoxins
Mastitis
Sheep
Staphylococcus spp.
Virulence factor genes
title_short Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
title_full Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
title_fullStr Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
title_full_unstemmed Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
title_sort Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
author Zafalon, Luiz F.
author_facet Zafalon, Luiz F.
Cunha, Maria L.R.S. [UNESP]
Brandão, Humberto M.
Mosqueira, Vanessa C.F.
Santana, Raul C.M.
Júnior, Waldomiro Barioni
Martins, Katheryne B. [UNESP]
Pilon, Lucas E. [UNESP]
author_role author
author2 Cunha, Maria L.R.S. [UNESP]
Brandão, Humberto M.
Mosqueira, Vanessa C.F.
Santana, Raul C.M.
Júnior, Waldomiro Barioni
Martins, Katheryne B. [UNESP]
Pilon, Lucas E. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
Universidade Federal de Ouro Preto
dc.contributor.author.fl_str_mv Zafalon, Luiz F.
Cunha, Maria L.R.S. [UNESP]
Brandão, Humberto M.
Mosqueira, Vanessa C.F.
Santana, Raul C.M.
Júnior, Waldomiro Barioni
Martins, Katheryne B. [UNESP]
Pilon, Lucas E. [UNESP]
dc.subject.por.fl_str_mv Antimicrobial treatment
Bacterioses
Biofilms
Coagulase-negative
Enterotoxins
Mastitis
Sheep
Staphylococcus spp.
Virulence factor genes
topic Antimicrobial treatment
Bacterioses
Biofilms
Coagulase-negative
Enterotoxins
Mastitis
Sheep
Staphylococcus spp.
Virulence factor genes
description Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:54:14Z
2018-12-11T16:54:14Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-5150-PVB-4984
Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018.
1678-5150
0100-736X
http://hdl.handle.net/11449/171173
10.1590/1678-5150-PVB-4984
S0100-736X2018000400579
2-s2.0-85049455998
S0100-736X2018000400579.pdf
url http://dx.doi.org/10.1590/1678-5150-PVB-4984
http://hdl.handle.net/11449/171173
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018.
1678-5150
0100-736X
10.1590/1678-5150-PVB-4984
S0100-736X2018000400579
2-s2.0-85049455998
S0100-736X2018000400579.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 579-585
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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