Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-5150-PVB-4984 http://hdl.handle.net/11449/171173 |
Resumo: | Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep. |
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Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheepAntimicrobial treatmentBacteriosesBiofilmsCoagulase-negativeEnterotoxinsMastitisSheepStaphylococcus spp.Virulence factor genesCoagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Embrapa Pecuária Sudeste Rodovia Washington Luiz, Km 234, Cx. Postal 339Instituto de Biociências Universidade Estadual Paulista (Unesp), Distrito de Rubião JúniorEmbrapa Gado de Leite, Rua Eugênio do Nascimento 610Universidade Federal de Ouro Preto, Rua Costa Sena 171Programa de Pós-Graduação em Medicina Veterinária Preventiva Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato CastellaneInstituto de Biociências Universidade Estadual Paulista (Unesp), Distrito de Rubião JúniorPrograma de Pós-Graduação em Medicina Veterinária Preventiva Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato CastellaneFAPESP: 2012/23044-0Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade Federal de Ouro PretoZafalon, Luiz F.Cunha, Maria L.R.S. [UNESP]Brandão, Humberto M.Mosqueira, Vanessa C.F.Santana, Raul C.M.Júnior, Waldomiro BarioniMartins, Katheryne B. [UNESP]Pilon, Lucas E. [UNESP]2018-12-11T16:54:14Z2018-12-11T16:54:14Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article579-585application/pdfhttp://dx.doi.org/10.1590/1678-5150-PVB-4984Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018.1678-51500100-736Xhttp://hdl.handle.net/11449/17117310.1590/1678-5150-PVB-4984S0100-736X20180004005792-s2.0-85049455998S0100-736X2018000400579.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-11-07T06:12:04Zoai:repositorio.unesp.br:11449/171173Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:05:11.698988Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
title |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
spellingShingle |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep Zafalon, Luiz F. Antimicrobial treatment Bacterioses Biofilms Coagulase-negative Enterotoxins Mastitis Sheep Staphylococcus spp. Virulence factor genes |
title_short |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
title_full |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
title_fullStr |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
title_full_unstemmed |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
title_sort |
Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep |
author |
Zafalon, Luiz F. |
author_facet |
Zafalon, Luiz F. Cunha, Maria L.R.S. [UNESP] Brandão, Humberto M. Mosqueira, Vanessa C.F. Santana, Raul C.M. Júnior, Waldomiro Barioni Martins, Katheryne B. [UNESP] Pilon, Lucas E. [UNESP] |
author_role |
author |
author2 |
Cunha, Maria L.R.S. [UNESP] Brandão, Humberto M. Mosqueira, Vanessa C.F. Santana, Raul C.M. Júnior, Waldomiro Barioni Martins, Katheryne B. [UNESP] Pilon, Lucas E. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) Universidade Federal de Ouro Preto |
dc.contributor.author.fl_str_mv |
Zafalon, Luiz F. Cunha, Maria L.R.S. [UNESP] Brandão, Humberto M. Mosqueira, Vanessa C.F. Santana, Raul C.M. Júnior, Waldomiro Barioni Martins, Katheryne B. [UNESP] Pilon, Lucas E. [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial treatment Bacterioses Biofilms Coagulase-negative Enterotoxins Mastitis Sheep Staphylococcus spp. Virulence factor genes |
topic |
Antimicrobial treatment Bacterioses Biofilms Coagulase-negative Enterotoxins Mastitis Sheep Staphylococcus spp. Virulence factor genes |
description |
Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ß-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:14Z 2018-12-11T16:54:14Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-5150-PVB-4984 Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018. 1678-5150 0100-736X http://hdl.handle.net/11449/171173 10.1590/1678-5150-PVB-4984 S0100-736X2018000400579 2-s2.0-85049455998 S0100-736X2018000400579.pdf |
url |
http://dx.doi.org/10.1590/1678-5150-PVB-4984 http://hdl.handle.net/11449/171173 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 38, n. 4, p. 579-585, 2018. 1678-5150 0100-736X 10.1590/1678-5150-PVB-4984 S0100-736X2018000400579 2-s2.0-85049455998 S0100-736X2018000400579.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
579-585 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128753660854272 |