Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation

Detalhes bibliográficos
Autor(a) principal: Gomes, Eleni [UNESP]
Data de Publicação: 2009
Outros Autores: Leite, Rodrigo Simões Ribeiro [UNESP], Da Silva, Roberto [UNESP], Silva, Dênis [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2009/631942
http://hdl.handle.net/11449/71313
Resumo: An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50-55°C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca 2+ enhanced the stability of enzyme and its activity by 30%. The K m was 1.30 in absence of Ca 2+ and 1.16mg mL -1 in presence of this ion. In relation to the Vmax the presence of this ion increased from 1.76 to 2.07 μmol min -1mg -1. Copyright © 2009 Eleni Gomes et al.
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spelling Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentationPenicilliumPenicillium viridicatumAn exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50-55°C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca 2+ enhanced the stability of enzyme and its activity by 30%. The K m was 1.30 in absence of Ca 2+ and 1.16mg mL -1 in presence of this ion. In relation to the Vmax the presence of this ion increased from 1.76 to 2.07 μmol min -1mg -1. Copyright © 2009 Eleni Gomes et al.Laboratory of Biochemistry and Applied Microbiology, Ibilce Universidade Estadual Paulista (Unesp), Rua Cristovao Colombo, 2265, Jd. Nazareth, Sãao José do Rio Preto, SP, CEP 15054-000Laboratory of Biochemistry and Applied Microbiology, Ibilce Universidade Estadual Paulista (Unesp), Rua Cristovao Colombo, 2265, Jd. Nazareth, Sãao José do Rio Preto, SP, CEP 15054-000Universidade Estadual Paulista (Unesp)Gomes, Eleni [UNESP]Leite, Rodrigo Simões Ribeiro [UNESP]Da Silva, Roberto [UNESP]Silva, Dênis [UNESP]2014-05-27T11:24:04Z2014-05-27T11:24:04Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2009/631942International Journal of Microbiology.1687-918X1687-9198http://hdl.handle.net/11449/7131310.1155/2009/6319422-s2.0-800525064112-s2.0-80052506411.pdf70912417428519209424175688206545Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Microbiology0,662info:eu-repo/semantics/openAccess2024-01-08T06:28:39Zoai:repositorio.unesp.br:11449/71313Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:28:25.815904Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
title Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
spellingShingle Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
Gomes, Eleni [UNESP]
Penicillium
Penicillium viridicatum
title_short Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
title_full Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
title_fullStr Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
title_full_unstemmed Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
title_sort Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation
author Gomes, Eleni [UNESP]
author_facet Gomes, Eleni [UNESP]
Leite, Rodrigo Simões Ribeiro [UNESP]
Da Silva, Roberto [UNESP]
Silva, Dênis [UNESP]
author_role author
author2 Leite, Rodrigo Simões Ribeiro [UNESP]
Da Silva, Roberto [UNESP]
Silva, Dênis [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gomes, Eleni [UNESP]
Leite, Rodrigo Simões Ribeiro [UNESP]
Da Silva, Roberto [UNESP]
Silva, Dênis [UNESP]
dc.subject.por.fl_str_mv Penicillium
Penicillium viridicatum
topic Penicillium
Penicillium viridicatum
description An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50-55°C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca 2+ enhanced the stability of enzyme and its activity by 30%. The K m was 1.30 in absence of Ca 2+ and 1.16mg mL -1 in presence of this ion. In relation to the Vmax the presence of this ion increased from 1.76 to 2.07 μmol min -1mg -1. Copyright © 2009 Eleni Gomes et al.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2014-05-27T11:24:04Z
2014-05-27T11:24:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2009/631942
International Journal of Microbiology.
1687-918X
1687-9198
http://hdl.handle.net/11449/71313
10.1155/2009/631942
2-s2.0-80052506411
2-s2.0-80052506411.pdf
7091241742851920
9424175688206545
url http://dx.doi.org/10.1155/2009/631942
http://hdl.handle.net/11449/71313
identifier_str_mv International Journal of Microbiology.
1687-918X
1687-9198
10.1155/2009/631942
2-s2.0-80052506411
2-s2.0-80052506411.pdf
7091241742851920
9424175688206545
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Microbiology
0,662
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129430065774592

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