Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA

Detalhes bibliográficos
Autor(a) principal: Silva, Duilio M. Z. A. [UNESP]
Data de Publicação: 2015
Outros Autores: Utsunomia, Ricardo [UNESP], Pansonato-Alves, Jose C. [UNESP], Oliveira, Claudio [UNESP], Foresti, Fausto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000438813
http://hdl.handle.net/11449/160866
Resumo: Astyanax is a genus of Characidae fishes currently composed of 155 valid species. Previous cytogenetic studies revealed high chromosomal diversification among them, and several studies have been performed using traditional cytogenetic techniques to investigate karyotypes and chromosomal locations of 18S and 5S rDNA genes. However, only a few studies are currently available about other repetitive sequences. Here, the chromosomal location of small nuclear RNA genes, identified as U1 and U2 snRNA clusters, was established and compared to the distribution of 5S rDNA and histone clusters in 5 Astyanax species (A. paranae, A. fasciatus, A. bockmanni, A. altiparanae, and A. jordani) using FISH. The cytogenetic mapping of U1 and U2 snRNA demonstrated a conserved pattern in the number of sites per genome independent of the location in Astyanax species. The location of the U1 snRNA gene was frequently associated with 5S rDNA sequences, indicating a possible interaction between the distinct repetitive DNA families. Finally, comparisons involving the location of U1 and U2 snRNA clusters in the chromosomes of Astyanax species revealed a very diverse pattern, suggesting that many rearrangements have occurred during the diversification process of this group. (C) 2015 S. Karger AG, Basel
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spelling Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNAFish cytogeneticsGenome evolutionHistone genesrDNAU snRNAAstyanax is a genus of Characidae fishes currently composed of 155 valid species. Previous cytogenetic studies revealed high chromosomal diversification among them, and several studies have been performed using traditional cytogenetic techniques to investigate karyotypes and chromosomal locations of 18S and 5S rDNA genes. However, only a few studies are currently available about other repetitive sequences. Here, the chromosomal location of small nuclear RNA genes, identified as U1 and U2 snRNA clusters, was established and compared to the distribution of 5S rDNA and histone clusters in 5 Astyanax species (A. paranae, A. fasciatus, A. bockmanni, A. altiparanae, and A. jordani) using FISH. The cytogenetic mapping of U1 and U2 snRNA demonstrated a conserved pattern in the number of sites per genome independent of the location in Astyanax species. The location of the U1 snRNA gene was frequently associated with 5S rDNA sequences, indicating a possible interaction between the distinct repetitive DNA families. Finally, comparisons involving the location of U1 and U2 snRNA clusters in the chromosomes of Astyanax species revealed a very diverse pattern, suggesting that many rearrangements have occurred during the diversification process of this group. (C) 2015 S. Karger AG, BaselUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilKargerUniversidade Estadual Paulista (Unesp)Silva, Duilio M. Z. A. [UNESP]Utsunomia, Ricardo [UNESP]Pansonato-Alves, Jose C. [UNESP]Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2018-11-26T16:17:03Z2018-11-26T16:17:03Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article144-152application/pdfhttp://dx.doi.org/10.1159/000438813Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 144-152, 2015.1424-8581http://hdl.handle.net/11449/16086610.1159/000438813WOS:000361848600008WOS000361848600008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytogenetic And Genome Research0,748info:eu-repo/semantics/openAccess2023-10-10T06:04:47Zoai:repositorio.unesp.br:11449/160866Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:31:08.408987Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
title Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
spellingShingle Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
Silva, Duilio M. Z. A. [UNESP]
Fish cytogenetics
Genome evolution
Histone genes
rDNA
U snRNA
title_short Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
title_full Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
title_fullStr Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
title_full_unstemmed Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
title_sort Chromosomal Mapping of Repetitive DNA Sequences in Five Species of Astyanax (Characiformes, Characidae) Reveals Independent Location of U1 and U2 snRNA Sites and Association of U1 snRNA and 5S rDNA
author Silva, Duilio M. Z. A. [UNESP]
author_facet Silva, Duilio M. Z. A. [UNESP]
Utsunomia, Ricardo [UNESP]
Pansonato-Alves, Jose C. [UNESP]
Oliveira, Claudio [UNESP]
Foresti, Fausto [UNESP]
author_role author
author2 Utsunomia, Ricardo [UNESP]
Pansonato-Alves, Jose C. [UNESP]
Oliveira, Claudio [UNESP]
Foresti, Fausto [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Duilio M. Z. A. [UNESP]
Utsunomia, Ricardo [UNESP]
Pansonato-Alves, Jose C. [UNESP]
Oliveira, Claudio [UNESP]
Foresti, Fausto [UNESP]
dc.subject.por.fl_str_mv Fish cytogenetics
Genome evolution
Histone genes
rDNA
U snRNA
topic Fish cytogenetics
Genome evolution
Histone genes
rDNA
U snRNA
description Astyanax is a genus of Characidae fishes currently composed of 155 valid species. Previous cytogenetic studies revealed high chromosomal diversification among them, and several studies have been performed using traditional cytogenetic techniques to investigate karyotypes and chromosomal locations of 18S and 5S rDNA genes. However, only a few studies are currently available about other repetitive sequences. Here, the chromosomal location of small nuclear RNA genes, identified as U1 and U2 snRNA clusters, was established and compared to the distribution of 5S rDNA and histone clusters in 5 Astyanax species (A. paranae, A. fasciatus, A. bockmanni, A. altiparanae, and A. jordani) using FISH. The cytogenetic mapping of U1 and U2 snRNA demonstrated a conserved pattern in the number of sites per genome independent of the location in Astyanax species. The location of the U1 snRNA gene was frequently associated with 5S rDNA sequences, indicating a possible interaction between the distinct repetitive DNA families. Finally, comparisons involving the location of U1 and U2 snRNA clusters in the chromosomes of Astyanax species revealed a very diverse pattern, suggesting that many rearrangements have occurred during the diversification process of this group. (C) 2015 S. Karger AG, Basel
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-11-26T16:17:03Z
2018-11-26T16:17:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000438813
Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 144-152, 2015.
1424-8581
http://hdl.handle.net/11449/160866
10.1159/000438813
WOS:000361848600008
WOS000361848600008.pdf
url http://dx.doi.org/10.1159/000438813
http://hdl.handle.net/11449/160866
identifier_str_mv Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 144-152, 2015.
1424-8581
10.1159/000438813
WOS:000361848600008
WOS000361848600008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytogenetic And Genome Research
0,748
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 144-152
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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