Inside-out vs. standard vein graft to repair a sensory nerve in rats
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/(SICI)1097-0185(19991101)256:3<227 http://hdl.handle.net/11449/65887 |
Resumo: | Nerve regeneration in a sensory nerve was obtained by the application of different techniques: inside-out vein graft (IOVG group) and standard vein graft (SVG group). These techniques provide a good microenvironment for axon regeneration in motor nerves, but their efficiency for regeneration of sensory nerves is controversial. The saphenous nerve was sectioned and repaired by the inside-out and standard vein graft techniques in rats. After 4, 12, and 20 weeks the graft and the distal stump were observed under electron microscopy. In each studied period, the pattern, diameters, and thickness of the myelin sheaths of the regenerated axons were measured in the graft and distal stump. A comparative study about the regenerated nerve fibers by these two different techniques was performed. Regenerated nerve fibers were prominent in both vein grafts 4 weeks after the surgical procedures. On the other hand, in the distal stump, regenerated nerve fibers were observed only from 12 weeks. In both inside-out vein graft and standard vein graft statistical difference was not observed about the diameters and thickness of the myelinated fibers after 20 weeks. On the other hand, the inside-out group had greater regenerated axon number when compared to the standard group. There is a capillary invasion in both graft and distal stump, especially in the IOVG group. The regenerated axons follow these capillaries all the time like satellite microfascicles. After 20 weeks, the diameters of regenerated fibers repaired by the standard vein graft technique were closer to the normal fibers compared to the inside-out vein graft. On the other hand, the pattern of these regenerated axons was better in the IOVG group. |
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Inside-out vs. standard vein graft to repair a sensory nerve in ratsNerve regenerationRatVein graftanimal experimentanimal tissuecapillary proliferationelectron microscopymalemyelin sheathnerve fiber regenerationnerve regenerationnerve transectionnonhumanpriority journalratsaphenous nervetechniquevein graftvein wallAnimalsAxonsDisease Models, AnimalImage Processing, Computer-AssistedJugular VeinsMaleNerve FibersNerve RegenerationPeripheral NervesRatsRats, WistarSaphenous VeinTransplantation, AutologousAnimaliaNerve regeneration in a sensory nerve was obtained by the application of different techniques: inside-out vein graft (IOVG group) and standard vein graft (SVG group). These techniques provide a good microenvironment for axon regeneration in motor nerves, but their efficiency for regeneration of sensory nerves is controversial. The saphenous nerve was sectioned and repaired by the inside-out and standard vein graft techniques in rats. After 4, 12, and 20 weeks the graft and the distal stump were observed under electron microscopy. In each studied period, the pattern, diameters, and thickness of the myelin sheaths of the regenerated axons were measured in the graft and distal stump. A comparative study about the regenerated nerve fibers by these two different techniques was performed. Regenerated nerve fibers were prominent in both vein grafts 4 weeks after the surgical procedures. On the other hand, in the distal stump, regenerated nerve fibers were observed only from 12 weeks. In both inside-out vein graft and standard vein graft statistical difference was not observed about the diameters and thickness of the myelinated fibers after 20 weeks. On the other hand, the inside-out group had greater regenerated axon number when compared to the standard group. There is a capillary invasion in both graft and distal stump, especially in the IOVG group. The regenerated axons follow these capillaries all the time like satellite microfascicles. After 20 weeks, the diameters of regenerated fibers repaired by the standard vein graft technique were closer to the normal fibers compared to the inside-out vein graft. On the other hand, the pattern of these regenerated axons was better in the IOVG group.Department of Anatomy IB/UNESP/Botucatu, São PauloDepartment of Morphology IB/UNESP/Botucatu, São PauloDepartment of Biostatistics IB/UNESP/Botucatu, São PauloDepartment of Anatomy IB/UNESP/Botucatu, 18618-000 Rubião Júnior, São PauloDepartment of Anatomy IB/UNESP/Botucatu, São PauloDepartment of Morphology IB/UNESP/Botucatu, São PauloDepartment of Biostatistics IB/UNESP/Botucatu, São PauloDepartment of Anatomy IB/UNESP/Botucatu, 18618-000 Rubião Júnior, São PauloUniversidade Estadual Paulista (Unesp)Ferrari, Fábio [UNESP]De Rodrigues, Antonio Castro [UNESP]Malvezzi, Cristiane Karina [UNESP]Silva, Maeli Dal Pai [UNESP]Padovani, Carlos Roberto [UNESP]2014-05-27T11:19:47Z2014-05-27T11:19:47Z1999-11-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article227-232application/pdfhttp://dx.doi.org/10.1002/(SICI)1097-0185(19991101)256:3<227Anatomical Record, v. 256, n. 3, p. 227-232, 1999.0003-276Xhttp://hdl.handle.net/11449/6588710.1002/(SICI)1097-0185(19991101)256:3<227WOS:0000833558000022-s2.0-00327028412-s2.0-0032702841.pdf8727897080522289Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnatomical Recordinfo:eu-repo/semantics/openAccess2024-01-26T06:28:39Zoai:repositorio.unesp.br:11449/65887Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:04.047448Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
title |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
spellingShingle |
Inside-out vs. standard vein graft to repair a sensory nerve in rats Ferrari, Fábio [UNESP] Nerve regeneration Rat Vein graft animal experiment animal tissue capillary proliferation electron microscopy male myelin sheath nerve fiber regeneration nerve regeneration nerve transection nonhuman priority journal rat saphenous nerve technique vein graft vein wall Animals Axons Disease Models, Animal Image Processing, Computer-Assisted Jugular Veins Male Nerve Fibers Nerve Regeneration Peripheral Nerves Rats Rats, Wistar Saphenous Vein Transplantation, Autologous Animalia |
title_short |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
title_full |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
title_fullStr |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
title_full_unstemmed |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
title_sort |
Inside-out vs. standard vein graft to repair a sensory nerve in rats |
author |
Ferrari, Fábio [UNESP] |
author_facet |
Ferrari, Fábio [UNESP] De Rodrigues, Antonio Castro [UNESP] Malvezzi, Cristiane Karina [UNESP] Silva, Maeli Dal Pai [UNESP] Padovani, Carlos Roberto [UNESP] |
author_role |
author |
author2 |
De Rodrigues, Antonio Castro [UNESP] Malvezzi, Cristiane Karina [UNESP] Silva, Maeli Dal Pai [UNESP] Padovani, Carlos Roberto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferrari, Fábio [UNESP] De Rodrigues, Antonio Castro [UNESP] Malvezzi, Cristiane Karina [UNESP] Silva, Maeli Dal Pai [UNESP] Padovani, Carlos Roberto [UNESP] |
dc.subject.por.fl_str_mv |
Nerve regeneration Rat Vein graft animal experiment animal tissue capillary proliferation electron microscopy male myelin sheath nerve fiber regeneration nerve regeneration nerve transection nonhuman priority journal rat saphenous nerve technique vein graft vein wall Animals Axons Disease Models, Animal Image Processing, Computer-Assisted Jugular Veins Male Nerve Fibers Nerve Regeneration Peripheral Nerves Rats Rats, Wistar Saphenous Vein Transplantation, Autologous Animalia |
topic |
Nerve regeneration Rat Vein graft animal experiment animal tissue capillary proliferation electron microscopy male myelin sheath nerve fiber regeneration nerve regeneration nerve transection nonhuman priority journal rat saphenous nerve technique vein graft vein wall Animals Axons Disease Models, Animal Image Processing, Computer-Assisted Jugular Veins Male Nerve Fibers Nerve Regeneration Peripheral Nerves Rats Rats, Wistar Saphenous Vein Transplantation, Autologous Animalia |
description |
Nerve regeneration in a sensory nerve was obtained by the application of different techniques: inside-out vein graft (IOVG group) and standard vein graft (SVG group). These techniques provide a good microenvironment for axon regeneration in motor nerves, but their efficiency for regeneration of sensory nerves is controversial. The saphenous nerve was sectioned and repaired by the inside-out and standard vein graft techniques in rats. After 4, 12, and 20 weeks the graft and the distal stump were observed under electron microscopy. In each studied period, the pattern, diameters, and thickness of the myelin sheaths of the regenerated axons were measured in the graft and distal stump. A comparative study about the regenerated nerve fibers by these two different techniques was performed. Regenerated nerve fibers were prominent in both vein grafts 4 weeks after the surgical procedures. On the other hand, in the distal stump, regenerated nerve fibers were observed only from 12 weeks. In both inside-out vein graft and standard vein graft statistical difference was not observed about the diameters and thickness of the myelinated fibers after 20 weeks. On the other hand, the inside-out group had greater regenerated axon number when compared to the standard group. There is a capillary invasion in both graft and distal stump, especially in the IOVG group. The regenerated axons follow these capillaries all the time like satellite microfascicles. After 20 weeks, the diameters of regenerated fibers repaired by the standard vein graft technique were closer to the normal fibers compared to the inside-out vein graft. On the other hand, the pattern of these regenerated axons was better in the IOVG group. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-11-09 2014-05-27T11:19:47Z 2014-05-27T11:19:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/(SICI)1097-0185(19991101)256:3<227 Anatomical Record, v. 256, n. 3, p. 227-232, 1999. 0003-276X http://hdl.handle.net/11449/65887 10.1002/(SICI)1097-0185(19991101)256:3<227 WOS:000083355800002 2-s2.0-0032702841 2-s2.0-0032702841.pdf 8727897080522289 |
url |
http://dx.doi.org/10.1002/(SICI)1097-0185(19991101)256:3<227 http://hdl.handle.net/11449/65887 |
identifier_str_mv |
Anatomical Record, v. 256, n. 3, p. 227-232, 1999. 0003-276X 10.1002/(SICI)1097-0185(19991101)256:3<227 WOS:000083355800002 2-s2.0-0032702841 2-s2.0-0032702841.pdf 8727897080522289 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anatomical Record |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
227-232 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129570974466048 |