A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus

Detalhes bibliográficos
Autor(a) principal: Urzêdo Leão, E. [UNESP]
Data de Publicação: 2016
Outros Autores: Tavella, L., Krause-Sakate, R. [UNESP], Turina, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4454/JPP.V98I2.018
http://hdl.handle.net/11449/228157
Resumo: Groundnut ringspot virus (GRSV) is a tospovirus species transmitted in a circulative propagative manner by several thrips species. Traditionally, GRSV is detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), so this paper describes a rapid diagnostic system for the reliable detection of GRSV in plants and individual thrips by real-time RT-PCR. Watermelon GRSV infected leaves and fruits, and individual thrips (Frankliniella schultzei) collected from flowers on infected watermelons were used in the test. We designed a GRSV TaqMan assay to efficiently detect GRSV in all samples. The method had high specificity and could distinguish GRSV from the other tospovirus species, like Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV) and Zucchini lethal chlorosis virus (ZLCV). The test will help us to collect epidemiological data for GRSV in plants and thrips worldwide.
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spelling A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virusFrankliniella schultzeiGRSVReal-time RT-PCRTospovirusGroundnut ringspot virus (GRSV) is a tospovirus species transmitted in a circulative propagative manner by several thrips species. Traditionally, GRSV is detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), so this paper describes a rapid diagnostic system for the reliable detection of GRSV in plants and individual thrips by real-time RT-PCR. Watermelon GRSV infected leaves and fruits, and individual thrips (Frankliniella schultzei) collected from flowers on infected watermelons were used in the test. We designed a GRSV TaqMan assay to efficiently detect GRSV in all samples. The method had high specificity and could distinguish GRSV from the other tospovirus species, like Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV) and Zucchini lethal chlorosis virus (ZLCV). The test will help us to collect epidemiological data for GRSV in plants and thrips worldwide.Department of Plant Protection Faculdade de Ciências Agronômicas Universidade Estadual de São Paulo, Rua Dr. José Barbosa de Barros 1780Dipartimento di Scienze Agrarie Forestali e Alimentari ULF Entomologia Generale e Applicata University of Turin, Largo Paolo Braccini 2Istituto per la Protezione Sostenibile delle Piante C.N.R, Strada delle Cacce 73Department of Plant Protection Faculdade de Ciências Agronômicas Universidade Estadual de São Paulo, Rua Dr. José Barbosa de Barros 1780Universidade Estadual Paulista (UNESP)University of TurinC.N.RUrzêdo Leão, E. [UNESP]Tavella, L.Krause-Sakate, R. [UNESP]Turina, M.2022-04-29T07:35:50Z2022-04-29T07:35:50Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4454/JPP.V98I2.018Journal of Plant Pathology, v. 98, n. 2, 2016.1125-4653http://hdl.handle.net/11449/22815710.4454/JPP.V98I2.0182-s2.0-84971472348Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Pathologyinfo:eu-repo/semantics/openAccess2024-04-30T18:07:43Zoai:repositorio.unesp.br:11449/228157Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:42:25.386846Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
title A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
spellingShingle A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
Urzêdo Leão, E. [UNESP]
Frankliniella schultzei
GRSV
Real-time RT-PCR
Tospovirus
title_short A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
title_full A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
title_fullStr A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
title_full_unstemmed A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
title_sort A TaqMan real – Time RT - PCR assay for detection of Groundnut ringspot virus
author Urzêdo Leão, E. [UNESP]
author_facet Urzêdo Leão, E. [UNESP]
Tavella, L.
Krause-Sakate, R. [UNESP]
Turina, M.
author_role author
author2 Tavella, L.
Krause-Sakate, R. [UNESP]
Turina, M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Turin
C.N.R
dc.contributor.author.fl_str_mv Urzêdo Leão, E. [UNESP]
Tavella, L.
Krause-Sakate, R. [UNESP]
Turina, M.
dc.subject.por.fl_str_mv Frankliniella schultzei
GRSV
Real-time RT-PCR
Tospovirus
topic Frankliniella schultzei
GRSV
Real-time RT-PCR
Tospovirus
description Groundnut ringspot virus (GRSV) is a tospovirus species transmitted in a circulative propagative manner by several thrips species. Traditionally, GRSV is detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), so this paper describes a rapid diagnostic system for the reliable detection of GRSV in plants and individual thrips by real-time RT-PCR. Watermelon GRSV infected leaves and fruits, and individual thrips (Frankliniella schultzei) collected from flowers on infected watermelons were used in the test. We designed a GRSV TaqMan assay to efficiently detect GRSV in all samples. The method had high specificity and could distinguish GRSV from the other tospovirus species, like Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV) and Zucchini lethal chlorosis virus (ZLCV). The test will help us to collect epidemiological data for GRSV in plants and thrips worldwide.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2022-04-29T07:35:50Z
2022-04-29T07:35:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4454/JPP.V98I2.018
Journal of Plant Pathology, v. 98, n. 2, 2016.
1125-4653
http://hdl.handle.net/11449/228157
10.4454/JPP.V98I2.018
2-s2.0-84971472348
url http://dx.doi.org/10.4454/JPP.V98I2.018
http://hdl.handle.net/11449/228157
identifier_str_mv Journal of Plant Pathology, v. 98, n. 2, 2016.
1125-4653
10.4454/JPP.V98I2.018
2-s2.0-84971472348
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Plant Pathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129349204836352

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