Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?

Detalhes bibliográficos
Autor(a) principal: Madeira, Fernanda Fernandez [UNESP]
Data de Publicação: 2021
Outros Autores: Delgado, Luiza Maria Grzyb [UNESP], Bittinelli, Isadora de Freitas [UNESP], de Oliveira, Jader [UNESP], Ravazi, Amanda [UNESP], dos Reis, Yago Visinho [UNESP], de Oliveira, Ana Beatriz Bortolozo [UNESP], Cristal, Daniel Cesaretto [UNESP], Galvão, Cleber, de Azeredo-Oliveira, Maria Tercília Vilela [UNESP], da Rosa, João Aristeu [UNESP], Alevi, Kaio Cesar Chaboli [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s13071-021-04988-9
http://hdl.handle.net/11449/229783
Resumo: Background: Triatoma sordida is one of the main Chagas disease vectors in Brazil. In addition to Brazil, this species has already been reported in Bolivia, Argentina, Paraguay, and Uruguay. It is hypothesized that the insects currently identified as T. sordida are a species subcomplex formed by three cytotypes (T. sordida sensu stricto [s.s.], T. sordida La Paz, and T. sordida Argentina). With the recent description of T. rosai from the Argentinean specimens, it became necessary to assess the taxonomic status of T. sordida from La Paz, Bolivia, since it was suggested that it may represent a new species, which has taxonomic, evolutionary, and epidemiological implications. Based on the above, we carried out molecular and experimental crossover studies to assess the specific status of T. sordida La Paz. Methods: To evaluate the pre- and postzygotic barriers between T. sordida La Paz and T. sordida s.s., experimental crosses and intercrosses between F1 hybrids and between F2 hybrids were conducted. In addition, cytogenetic analyses of the F1 and F2 hybrids were applied with an emphasis on the degree of pairing between the homeologous chromosomes, and morphological analyses of the male gonads were performed to evaluate the presence of gonadal dysgenesis. Lastly, the genetic distance between T. sordida La Paz and T. sordida s.s. was calculated for the CYTB, ND1, and ITS1 genes. Results: Regardless of the gene used, T. sordida La Paz showed low genetic distance compared to T. sordida s.s. (below 2%). Experimental crosses resulted in offspring for both directions, demonstrating that there are no prezygotic barriers installed between these allopatric populations. Furthermore, postzygotic barriers were not observed either (since the F1 × F1 and F2 × F2 intercrosses resulted in viable offspring). Morphological and cytogenetic analyses of the male gonads of the F1 and F2 offspring demonstrated that the testes were not atrophied and did not show chromosome pairing errors. Conclusion: Based on the low genetic distance (which configures intraspecific variation), associated with the absence of prezygotic and postzygotic reproductive barriers, we confirm that T. sordida La Paz represents only a chromosomal polymorphism of T. sordida s.s. Graphical abstract: [Figure not available: see fulltext.]
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spelling Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?Chagas disease vectorExperimental crossesGenetic distanceTriatominesBackground: Triatoma sordida is one of the main Chagas disease vectors in Brazil. In addition to Brazil, this species has already been reported in Bolivia, Argentina, Paraguay, and Uruguay. It is hypothesized that the insects currently identified as T. sordida are a species subcomplex formed by three cytotypes (T. sordida sensu stricto [s.s.], T. sordida La Paz, and T. sordida Argentina). With the recent description of T. rosai from the Argentinean specimens, it became necessary to assess the taxonomic status of T. sordida from La Paz, Bolivia, since it was suggested that it may represent a new species, which has taxonomic, evolutionary, and epidemiological implications. Based on the above, we carried out molecular and experimental crossover studies to assess the specific status of T. sordida La Paz. Methods: To evaluate the pre- and postzygotic barriers between T. sordida La Paz and T. sordida s.s., experimental crosses and intercrosses between F1 hybrids and between F2 hybrids were conducted. In addition, cytogenetic analyses of the F1 and F2 hybrids were applied with an emphasis on the degree of pairing between the homeologous chromosomes, and morphological analyses of the male gonads were performed to evaluate the presence of gonadal dysgenesis. Lastly, the genetic distance between T. sordida La Paz and T. sordida s.s. was calculated for the CYTB, ND1, and ITS1 genes. Results: Regardless of the gene used, T. sordida La Paz showed low genetic distance compared to T. sordida s.s. (below 2%). Experimental crosses resulted in offspring for both directions, demonstrating that there are no prezygotic barriers installed between these allopatric populations. Furthermore, postzygotic barriers were not observed either (since the F1 × F1 and F2 × F2 intercrosses resulted in viable offspring). Morphological and cytogenetic analyses of the male gonads of the F1 and F2 offspring demonstrated that the testes were not atrophied and did not show chromosome pairing errors. Conclusion: Based on the low genetic distance (which configures intraspecific variation), associated with the absence of prezygotic and postzygotic reproductive barriers, we confirm that T. sordida La Paz represents only a chromosomal polymorphism of T. sordida s.s. Graphical abstract: [Figure not available: see fulltext.]Laboratório de Biologia Celular Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de Biociências Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião JúniorLaboratório de Parasitologia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1Laboratório de Entomologia em Saúde Pública Departamento de Epidemiologia Faculdade de Saúde Pública Universidade de São Paulo, Av. Dr. Arnaldo 715Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Pavilhão Rocha Lima, sala 505Laboratório de Biologia Celular Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de Biociências Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Instituto de Biociências Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião JúniorLaboratório de Parasitologia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 1Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Instituto Oswaldo Cruz (FIOCRUZ)Madeira, Fernanda Fernandez [UNESP]Delgado, Luiza Maria Grzyb [UNESP]Bittinelli, Isadora de Freitas [UNESP]de Oliveira, Jader [UNESP]Ravazi, Amanda [UNESP]dos Reis, Yago Visinho [UNESP]de Oliveira, Ana Beatriz Bortolozo [UNESP]Cristal, Daniel Cesaretto [UNESP]Galvão, Cleberde Azeredo-Oliveira, Maria Tercília Vilela [UNESP]da Rosa, João Aristeu [UNESP]Alevi, Kaio Cesar Chaboli [UNESP]2022-04-29T08:35:44Z2022-04-29T08:35:44Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13071-021-04988-9Parasites and Vectors, v. 14, n. 1, 2021.1756-3305http://hdl.handle.net/11449/22978310.1186/s13071-021-04988-92-s2.0-85117882826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasites and Vectorsinfo:eu-repo/semantics/openAccess2022-04-29T08:35:44Zoai:repositorio.unesp.br:11449/229783Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:53:58.268358Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
title Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
spellingShingle Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
Madeira, Fernanda Fernandez [UNESP]
Chagas disease vector
Experimental crosses
Genetic distance
Triatomines
title_short Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
title_full Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
title_fullStr Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
title_full_unstemmed Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
title_sort Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?
author Madeira, Fernanda Fernandez [UNESP]
author_facet Madeira, Fernanda Fernandez [UNESP]
Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
author_role author
author2 Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Instituto Oswaldo Cruz (FIOCRUZ)
dc.contributor.author.fl_str_mv Madeira, Fernanda Fernandez [UNESP]
Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
dc.subject.por.fl_str_mv Chagas disease vector
Experimental crosses
Genetic distance
Triatomines
topic Chagas disease vector
Experimental crosses
Genetic distance
Triatomines
description Background: Triatoma sordida is one of the main Chagas disease vectors in Brazil. In addition to Brazil, this species has already been reported in Bolivia, Argentina, Paraguay, and Uruguay. It is hypothesized that the insects currently identified as T. sordida are a species subcomplex formed by three cytotypes (T. sordida sensu stricto [s.s.], T. sordida La Paz, and T. sordida Argentina). With the recent description of T. rosai from the Argentinean specimens, it became necessary to assess the taxonomic status of T. sordida from La Paz, Bolivia, since it was suggested that it may represent a new species, which has taxonomic, evolutionary, and epidemiological implications. Based on the above, we carried out molecular and experimental crossover studies to assess the specific status of T. sordida La Paz. Methods: To evaluate the pre- and postzygotic barriers between T. sordida La Paz and T. sordida s.s., experimental crosses and intercrosses between F1 hybrids and between F2 hybrids were conducted. In addition, cytogenetic analyses of the F1 and F2 hybrids were applied with an emphasis on the degree of pairing between the homeologous chromosomes, and morphological analyses of the male gonads were performed to evaluate the presence of gonadal dysgenesis. Lastly, the genetic distance between T. sordida La Paz and T. sordida s.s. was calculated for the CYTB, ND1, and ITS1 genes. Results: Regardless of the gene used, T. sordida La Paz showed low genetic distance compared to T. sordida s.s. (below 2%). Experimental crosses resulted in offspring for both directions, demonstrating that there are no prezygotic barriers installed between these allopatric populations. Furthermore, postzygotic barriers were not observed either (since the F1 × F1 and F2 × F2 intercrosses resulted in viable offspring). Morphological and cytogenetic analyses of the male gonads of the F1 and F2 offspring demonstrated that the testes were not atrophied and did not show chromosome pairing errors. Conclusion: Based on the low genetic distance (which configures intraspecific variation), associated with the absence of prezygotic and postzygotic reproductive barriers, we confirm that T. sordida La Paz represents only a chromosomal polymorphism of T. sordida s.s. Graphical abstract: [Figure not available: see fulltext.]
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-29T08:35:44Z
2022-04-29T08:35:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s13071-021-04988-9
Parasites and Vectors, v. 14, n. 1, 2021.
1756-3305
http://hdl.handle.net/11449/229783
10.1186/s13071-021-04988-9
2-s2.0-85117882826
url http://dx.doi.org/10.1186/s13071-021-04988-9
http://hdl.handle.net/11449/229783
identifier_str_mv Parasites and Vectors, v. 14, n. 1, 2021.
1756-3305
10.1186/s13071-021-04988-9
2-s2.0-85117882826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasites and Vectors
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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