Perivascular adipose tissue and vascular responses in healthy trained rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.sciencedirect.com/science/article/pii/S0024320515000582 http://hdl.handle.net/11449/128764 |
Resumo: | Aims: The importance of perivascular adipose tissue (PVAT) in vascular function has recently been recognized. The aim of the study was to investigate the effects of exercise training on anticontractile responses of periaortic adipose tissue.Main methods: Male Wistar rats were divided into sedentary (SD) and trained (TR). Running training was performed for 60 min/day, 5 days/week, for 8 weeks. Concentration-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (PHE) and serotonin (5-HT) were obtained in aortic rings without (PVAT-) or with (PVAT+) PVAT. The protein expressions of eNOS, AMPK alpha, pAMPKThr172 and mtTFA were determined in PVAT. The contents of adiponectin, leptin and TNF-alpha were evaluated systemically and locally.Key findings: The PVAT+ rings did not modify the relaxing responses to ACh and SNP whereas it showed anticontractile effects for both PHE and 5-HT agents in the SD and TR groups. The amount of PVAT was markedly reduced in TR (3.6 +/- 03 mg/mm) compared with SD (6.8 +/- 0.6 mg/mm). Increased protein expressions of eNOS, pAMPKThr172 and mtTFA were observed in PVAT from TR animals, without modifications in PVAT-derived adiponectin, leptin and TNF-alpha. Circulatory leptin levels were reduced in TR without changes in adiponectin.Significance: Our findings show that exercise training for 8 weeks did not alter the anticontractile effects induced by PVAT in rat-isolated aorta. Moreover, PVAT-derived adipokine, adiponectin and leptin levels were not different in trained healthy animals despite a significant metabolic adaptation and reduction in periaortic adipose tissue amount. (C) 2015 Elsevier Inc. All rights reserved. |
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Repositório Institucional da UNESP |
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Perivascular adipose tissue and vascular responses in healthy trained ratsAerobic exercise trainingVascular responsePerivascular adipose tissueAdipokinesAims: The importance of perivascular adipose tissue (PVAT) in vascular function has recently been recognized. The aim of the study was to investigate the effects of exercise training on anticontractile responses of periaortic adipose tissue.Main methods: Male Wistar rats were divided into sedentary (SD) and trained (TR). Running training was performed for 60 min/day, 5 days/week, for 8 weeks. Concentration-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (PHE) and serotonin (5-HT) were obtained in aortic rings without (PVAT-) or with (PVAT+) PVAT. The protein expressions of eNOS, AMPK alpha, pAMPKThr172 and mtTFA were determined in PVAT. The contents of adiponectin, leptin and TNF-alpha were evaluated systemically and locally.Key findings: The PVAT+ rings did not modify the relaxing responses to ACh and SNP whereas it showed anticontractile effects for both PHE and 5-HT agents in the SD and TR groups. The amount of PVAT was markedly reduced in TR (3.6 +/- 03 mg/mm) compared with SD (6.8 +/- 0.6 mg/mm). Increased protein expressions of eNOS, pAMPKThr172 and mtTFA were observed in PVAT from TR animals, without modifications in PVAT-derived adiponectin, leptin and TNF-alpha. Circulatory leptin levels were reduced in TR without changes in adiponectin.Significance: Our findings show that exercise training for 8 weeks did not alter the anticontractile effects induced by PVAT in rat-isolated aorta. Moreover, PVAT-derived adipokine, adiponectin and leptin levels were not different in trained healthy animals despite a significant metabolic adaptation and reduction in periaortic adipose tissue amount. (C) 2015 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Dept Phys Educ, Inst Biosci, Rio Claro, SP, BrazilUniv Estadual Campinas, UNICAMP, Inst Biol, Dept Struct &Funct Biol, Campinas, SP, BrazilUniv Estadual Campinas, UNICAMP, Sch Med Sci, Dept Pharmacol, Campinas, SP, BrazilUniv Estadual Paulista, UNESP, Departamento de Educação Física, Inst Biosci, Rio Claro, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Araujo, Hygor N. [UNESP]Valgas da Silva, Carmem P. [UNESP]Sponton, Amanda C. S.Clerici, Stefano P.Davel, Ana P. C.Antunes, EdsonZanesco, Angelina [UNESP]Delbin, Maria A.2015-10-21T13:13:16Z2015-10-21T13:13:16Z2015-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article79-87http://www.sciencedirect.com/science/article/pii/S0024320515000582Life Sciences, v. 125, p. 79-87, 2015.0024-3205http://hdl.handle.net/11449/12876410.1016/j.lfs.2014.12.032WOS:0003516594000124472007237545596Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciences3.2341,071info:eu-repo/semantics/openAccess2021-10-23T21:57:01Zoai:repositorio.unesp.br:11449/128764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:43:17.078715Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Perivascular adipose tissue and vascular responses in healthy trained rats |
title |
Perivascular adipose tissue and vascular responses in healthy trained rats |
spellingShingle |
Perivascular adipose tissue and vascular responses in healthy trained rats Araujo, Hygor N. [UNESP] Aerobic exercise training Vascular response Perivascular adipose tissue Adipokines |
title_short |
Perivascular adipose tissue and vascular responses in healthy trained rats |
title_full |
Perivascular adipose tissue and vascular responses in healthy trained rats |
title_fullStr |
Perivascular adipose tissue and vascular responses in healthy trained rats |
title_full_unstemmed |
Perivascular adipose tissue and vascular responses in healthy trained rats |
title_sort |
Perivascular adipose tissue and vascular responses in healthy trained rats |
author |
Araujo, Hygor N. [UNESP] |
author_facet |
Araujo, Hygor N. [UNESP] Valgas da Silva, Carmem P. [UNESP] Sponton, Amanda C. S. Clerici, Stefano P. Davel, Ana P. C. Antunes, Edson Zanesco, Angelina [UNESP] Delbin, Maria A. |
author_role |
author |
author2 |
Valgas da Silva, Carmem P. [UNESP] Sponton, Amanda C. S. Clerici, Stefano P. Davel, Ana P. C. Antunes, Edson Zanesco, Angelina [UNESP] Delbin, Maria A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Araujo, Hygor N. [UNESP] Valgas da Silva, Carmem P. [UNESP] Sponton, Amanda C. S. Clerici, Stefano P. Davel, Ana P. C. Antunes, Edson Zanesco, Angelina [UNESP] Delbin, Maria A. |
dc.subject.por.fl_str_mv |
Aerobic exercise training Vascular response Perivascular adipose tissue Adipokines |
topic |
Aerobic exercise training Vascular response Perivascular adipose tissue Adipokines |
description |
Aims: The importance of perivascular adipose tissue (PVAT) in vascular function has recently been recognized. The aim of the study was to investigate the effects of exercise training on anticontractile responses of periaortic adipose tissue.Main methods: Male Wistar rats were divided into sedentary (SD) and trained (TR). Running training was performed for 60 min/day, 5 days/week, for 8 weeks. Concentration-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (PHE) and serotonin (5-HT) were obtained in aortic rings without (PVAT-) or with (PVAT+) PVAT. The protein expressions of eNOS, AMPK alpha, pAMPKThr172 and mtTFA were determined in PVAT. The contents of adiponectin, leptin and TNF-alpha were evaluated systemically and locally.Key findings: The PVAT+ rings did not modify the relaxing responses to ACh and SNP whereas it showed anticontractile effects for both PHE and 5-HT agents in the SD and TR groups. The amount of PVAT was markedly reduced in TR (3.6 +/- 03 mg/mm) compared with SD (6.8 +/- 0.6 mg/mm). Increased protein expressions of eNOS, pAMPKThr172 and mtTFA were observed in PVAT from TR animals, without modifications in PVAT-derived adiponectin, leptin and TNF-alpha. Circulatory leptin levels were reduced in TR without changes in adiponectin.Significance: Our findings show that exercise training for 8 weeks did not alter the anticontractile effects induced by PVAT in rat-isolated aorta. Moreover, PVAT-derived adipokine, adiponectin and leptin levels were not different in trained healthy animals despite a significant metabolic adaptation and reduction in periaortic adipose tissue amount. (C) 2015 Elsevier Inc. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:13:16Z 2015-10-21T13:13:16Z 2015-03-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.sciencedirect.com/science/article/pii/S0024320515000582 Life Sciences, v. 125, p. 79-87, 2015. 0024-3205 http://hdl.handle.net/11449/128764 10.1016/j.lfs.2014.12.032 WOS:000351659400012 4472007237545596 |
url |
http://www.sciencedirect.com/science/article/pii/S0024320515000582 http://hdl.handle.net/11449/128764 |
identifier_str_mv |
Life Sciences, v. 125, p. 79-87, 2015. 0024-3205 10.1016/j.lfs.2014.12.032 WOS:000351659400012 4472007237545596 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences 3.234 1,071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
79-87 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128849719853056 |