Bronchoalveolar lavage (BAL) in tapirs (Tapirus terrestris) withMycobacterium tuberculosis

• Autor(a) principal: Biava, J. S. [UNESP]
• Data de Publicação: 2014
• Outros Autores: Gonçalves, R. C.[UNESP], Javorouski, M. L., Bonat, M., Lacerda, O., Murakami, P. S., Vilani, R. G. D. C., Biondo, A. W.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.scielo.br/scielo.php?pid=S0102-09352014000601643&script=sci_arttext&tlng=pt; http://hdl.handle.net/11449/127388
• topic: Tapirs; Diagnosis; Endoscopy; Tuberculosis; Antas; Diagnóstico; Endoscopia; Tuberculose

This paper describes the in vivo Bronchoalveolar lavage (BAL)technique by endoscopy in tapirs (Tapirus terrestris) with clinicalsigns of tuberculosis. The technique was performed in two tapirs, male and female,from Curitiba Zoo, Paraná, Brazil. A flexible endoscope and a polyethylene catheterwere used after the chemical restraint of the animals. For BAL technique, 60mL ofsaline 0.9% were infused with a polyethylene catheter, introduced by the endoscope'working channel, and 15mL of BAL were recovered, analyzed and submitted tocytocentrifugation. Slides were stained by Papanicolaou, periodic acid-Schiff (PAS)and Ziehl-Neelsen methods contained high quantity of inflammatory cells on lightmicroscopy (macrophages 27.5%, lymphocytes 0.5%, neutrophis 67% and eosinophis 5%).BAL samples were submitted to culture, bacilloscopy and PCR and were negative forboth animals. Based on this study, it was concluded that the bronchoalveolar lavagetechnique in tapirs is feasible, simple, noninvasive, practical and fast, providingan important clinical information in vivo regarding the functionalstatus of the lower respiratory tract.