Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil

Detalhes bibliográficos
Autor(a) principal: Mongruel, Anna Claudia Baumel [UNESP]
Data de Publicação: 2022
Outros Autores: Medici, Emília Patrícia, Canena, Ariel da Costa, Calchi, Ana Cláudia [UNESP], Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/microorganisms10030614
http://hdl.handle.net/11449/234256
Resumo: The lowland tapir (Tapirus terrestris) is the largest land mammal in Brazil and classified as a vulnerable species, according to the assessment of the risk of extinction. The present study aimed at investigating the occurrence and genetic diversity of hemoplasmas in free-ranging T. terrestris from the Brazilian Pantanal and Cerrado biomes. Blood samples were collected from 94 living and eight road-killed tapirs, totalizing 125 samples Conventional PCR targeting four different genes (16S rRNA, 23S rRNA, RNAse P, and dnaK) were performed, and the obtained sequences were submitted for phylogenetic, genotype diversity, and distance analyses. The association between hemoplasma positivity and possible risk variables (age, gender, and origin) was assessed. Out of 122 analyzed samples, 41 (41/122; 33.61% CI: 25.84–42.38%) were positive in the 16S rRNA-based PCR assay for hemoplasmas. Positivity for hemoplasmas did not differ between tapirs’ gender and age. Tapirs from Pantanal were 5.64 times more likely to present positive results for hemoplasmas when compared to tapirs sampled in Cerrado. BLASTn, phylogenetic, genotype diversity, and distance analyses performed herein showed that the sampled lowland tapirs might be infected by two genetically distinct hemoplasmas, namely ‘Candidatus Mycoplasma haematoterrestris’ and ‘Candidatus Mycoplasma haematotapirus’. While the former was positioned into “Mycoplasma haemofelis group” and closely related to ‘Candidatus Mycoplasma haematoparvum, the latter was positioned into “Mycoplasma suis group” and closely related to ‘Candidatus Mycoplasma haematobos’. The impact of both putative novel species on tapir health status should be investigated.
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spelling Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in BrazilCerradoHemotropic MycoplasmaLowland tapirsPantanalThe lowland tapir (Tapirus terrestris) is the largest land mammal in Brazil and classified as a vulnerable species, according to the assessment of the risk of extinction. The present study aimed at investigating the occurrence and genetic diversity of hemoplasmas in free-ranging T. terrestris from the Brazilian Pantanal and Cerrado biomes. Blood samples were collected from 94 living and eight road-killed tapirs, totalizing 125 samples Conventional PCR targeting four different genes (16S rRNA, 23S rRNA, RNAse P, and dnaK) were performed, and the obtained sequences were submitted for phylogenetic, genotype diversity, and distance analyses. The association between hemoplasma positivity and possible risk variables (age, gender, and origin) was assessed. Out of 122 analyzed samples, 41 (41/122; 33.61% CI: 25.84–42.38%) were positive in the 16S rRNA-based PCR assay for hemoplasmas. Positivity for hemoplasmas did not differ between tapirs’ gender and age. Tapirs from Pantanal were 5.64 times more likely to present positive results for hemoplasmas when compared to tapirs sampled in Cerrado. BLASTn, phylogenetic, genotype diversity, and distance analyses performed herein showed that the sampled lowland tapirs might be infected by two genetically distinct hemoplasmas, namely ‘Candidatus Mycoplasma haematoterrestris’ and ‘Candidatus Mycoplasma haematotapirus’. While the former was positioned into “Mycoplasma haemofelis group” and closely related to ‘Candidatus Mycoplasma haematoparvum, the latter was positioned into “Mycoplasma suis group” and closely related to ‘Candidatus Mycoplasma haematobos’. The impact of both putative novel species on tapir health status should be investigated.Universidade Estadual PaulistaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Immunoparasitology Laboratory Department of Pathology Theriogenology and One Health School of Agricultural and Veterinary Sciences São Paulo State University UNESP, SPIniciativa Nacional para a Conservação da Anta Brasileira (INCAB) Instituto de Pesquisas Ecológicas (IPÊ), MSEscola Superior de Conservação Ambiental e Sustentabilidade (ESCAS/IPÊ), SPTapir Specialist Group (TSG) International Union for Conservation of Nature (IUCN SSC), MSImmunoparasitology Laboratory Department of Pathology Theriogenology and One Health School of Agricultural and Veterinary Sciences São Paulo State University UNESP, SPFAPESP: 2018/02753-0FAPESP: 2019/26403-0FAPESP: 2020/12037-0CNPq: 303701/2021-8Universidade Estadual Paulista (UNESP)Instituto de Pesquisas Ecológicas (IPÊ)Escola Superior de Conservação Ambiental e Sustentabilidade (ESCAS/IPÊ)International Union for Conservation of Nature (IUCN SSC)Mongruel, Anna Claudia Baumel [UNESP]Medici, Emília PatríciaCanena, Ariel da CostaCalchi, Ana Cláudia [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2022-05-01T15:13:40Z2022-05-01T15:13:40Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms10030614Microorganisms, v. 10, n. 3, 2022.2076-2607http://hdl.handle.net/11449/23425610.3390/microorganisms100306142-s2.0-85126308858Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2024-06-07T13:02:17Zoai:repositorio.unesp.br:11449/234256Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:40:33.604814Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
title Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
spellingShingle Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
Mongruel, Anna Claudia Baumel [UNESP]
Cerrado
Hemotropic Mycoplasma
Lowland tapirs
Pantanal
title_short Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
title_full Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
title_fullStr Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
title_full_unstemmed Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
title_sort Expanding the Universe of Hemoplasmas: Multi-Locus Sequencing Reveals Putative Novel Hemoplasmas in Lowland Tapirs (Tapirus terrestris), the Largest Land Mammals in Brazil
author Mongruel, Anna Claudia Baumel [UNESP]
author_facet Mongruel, Anna Claudia Baumel [UNESP]
Medici, Emília Patrícia
Canena, Ariel da Costa
Calchi, Ana Cláudia [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 Medici, Emília Patrícia
Canena, Ariel da Costa
Calchi, Ana Cláudia [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Instituto de Pesquisas Ecológicas (IPÊ)
Escola Superior de Conservação Ambiental e Sustentabilidade (ESCAS/IPÊ)
International Union for Conservation of Nature (IUCN SSC)
dc.contributor.author.fl_str_mv Mongruel, Anna Claudia Baumel [UNESP]
Medici, Emília Patrícia
Canena, Ariel da Costa
Calchi, Ana Cláudia [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv Cerrado
Hemotropic Mycoplasma
Lowland tapirs
Pantanal
topic Cerrado
Hemotropic Mycoplasma
Lowland tapirs
Pantanal
description The lowland tapir (Tapirus terrestris) is the largest land mammal in Brazil and classified as a vulnerable species, according to the assessment of the risk of extinction. The present study aimed at investigating the occurrence and genetic diversity of hemoplasmas in free-ranging T. terrestris from the Brazilian Pantanal and Cerrado biomes. Blood samples were collected from 94 living and eight road-killed tapirs, totalizing 125 samples Conventional PCR targeting four different genes (16S rRNA, 23S rRNA, RNAse P, and dnaK) were performed, and the obtained sequences were submitted for phylogenetic, genotype diversity, and distance analyses. The association between hemoplasma positivity and possible risk variables (age, gender, and origin) was assessed. Out of 122 analyzed samples, 41 (41/122; 33.61% CI: 25.84–42.38%) were positive in the 16S rRNA-based PCR assay for hemoplasmas. Positivity for hemoplasmas did not differ between tapirs’ gender and age. Tapirs from Pantanal were 5.64 times more likely to present positive results for hemoplasmas when compared to tapirs sampled in Cerrado. BLASTn, phylogenetic, genotype diversity, and distance analyses performed herein showed that the sampled lowland tapirs might be infected by two genetically distinct hemoplasmas, namely ‘Candidatus Mycoplasma haematoterrestris’ and ‘Candidatus Mycoplasma haematotapirus’. While the former was positioned into “Mycoplasma haemofelis group” and closely related to ‘Candidatus Mycoplasma haematoparvum, the latter was positioned into “Mycoplasma suis group” and closely related to ‘Candidatus Mycoplasma haematobos’. The impact of both putative novel species on tapir health status should be investigated.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T15:13:40Z
2022-05-01T15:13:40Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/microorganisms10030614
Microorganisms, v. 10, n. 3, 2022.
2076-2607
http://hdl.handle.net/11449/234256
10.3390/microorganisms10030614
2-s2.0-85126308858
url http://dx.doi.org/10.3390/microorganisms10030614
http://hdl.handle.net/11449/234256
identifier_str_mv Microorganisms, v. 10, n. 3, 2022.
2076-2607
10.3390/microorganisms10030614
2-s2.0-85126308858
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
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instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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