Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment

Detalhes bibliográficos
Autor(a) principal: Oliveira, J. P. [UNESP]
Data de Publicação: 2016
Outros Autores: Kawanami, A. E. [UNESP], Silva, A. S.L. [UNESP], Chung, D. G. [UNESP], Werther, K. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.actatropica.2016.08.019
http://hdl.handle.net/11449/173489
Resumo: Leptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331 bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.
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spelling Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environmentLeptospiraPublic healthReptileTerrapinZoonosisLeptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331 bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nUniversidade Federal do Oeste do Pará (UFOPA) Instituto de Biodiversidade e Floresta (IBEF), Rua Vera Paz s/n, Bairro Salé, 68035-110Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Instituto de Biodiversidade e Floresta (IBEF)Oliveira, J. P. [UNESP]Kawanami, A. E. [UNESP]Silva, A. S.L. [UNESP]Chung, D. G. [UNESP]Werther, K. [UNESP]2018-12-11T17:05:51Z2018-12-11T17:05:51Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article165-168application/pdfhttp://dx.doi.org/10.1016/j.actatropica.2016.08.019Acta Tropica, v. 164, p. 165-168.1873-62540001-706Xhttp://hdl.handle.net/11449/17348910.1016/j.actatropica.2016.08.0192-s2.0-849880082582-s2.0-84988008258.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2024-06-07T13:02:16Zoai:repositorio.unesp.br:11449/173489Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:43.324081Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
title Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
spellingShingle Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
Oliveira, J. P. [UNESP]
Leptospira
Public health
Reptile
Terrapin
Zoonosis
title_short Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
title_full Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
title_fullStr Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
title_full_unstemmed Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
title_sort Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment
author Oliveira, J. P. [UNESP]
author_facet Oliveira, J. P. [UNESP]
Kawanami, A. E. [UNESP]
Silva, A. S.L. [UNESP]
Chung, D. G. [UNESP]
Werther, K. [UNESP]
author_role author
author2 Kawanami, A. E. [UNESP]
Silva, A. S.L. [UNESP]
Chung, D. G. [UNESP]
Werther, K. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Biodiversidade e Floresta (IBEF)
dc.contributor.author.fl_str_mv Oliveira, J. P. [UNESP]
Kawanami, A. E. [UNESP]
Silva, A. S.L. [UNESP]
Chung, D. G. [UNESP]
Werther, K. [UNESP]
dc.subject.por.fl_str_mv Leptospira
Public health
Reptile
Terrapin
Zoonosis
topic Leptospira
Public health
Reptile
Terrapin
Zoonosis
description Leptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331 bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
2018-12-11T17:05:51Z
2018-12-11T17:05:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.actatropica.2016.08.019
Acta Tropica, v. 164, p. 165-168.
1873-6254
0001-706X
http://hdl.handle.net/11449/173489
10.1016/j.actatropica.2016.08.019
2-s2.0-84988008258
2-s2.0-84988008258.pdf
url http://dx.doi.org/10.1016/j.actatropica.2016.08.019
http://hdl.handle.net/11449/173489
identifier_str_mv Acta Tropica, v. 164, p. 165-168.
1873-6254
0001-706X
10.1016/j.actatropica.2016.08.019
2-s2.0-84988008258
2-s2.0-84988008258.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Tropica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 165-168
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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