Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol9-2gmr731 http://hdl.handle.net/11449/71787 |
Resumo: | Complete blood counts and hemoglobin isoform data were gathered from 36 specimens of the turtle species Phrynops geoffroanus from the northwestern region of São Paulo State, Brazil. They were collected in an urban area. The hemoglobin profiles were obtained after red blood cell lysis and by electrophoretic migration in alkaline pH, acid pH, and neutral pH buffer. The hemoglobin components were confirmed using high-performance liquid chromatography (HPLC). Erythrogram analysis included hematocrit, total hemoglobin concentration, total red blood cell count, and red blood cell indices. The leukogram included a total white blood cell count and a calculation of the percent values of neutrophils, lymphocytes, monocytes, basophils, eosinophils, heterophils, and azurophils. HPLC analysis revealed three hemoglobin components; the first with a concentration of 5.5%, the second was a major component with an average concentration of 67.1%, and the third with a concentration of 28.5%. The hematological profile obtained for these specimens allowed us to establish a pattern for P. geoffroanus in São Paulo State Northwestern region. The average hematocrit values were 22.5% for females and 24.0% for males. For total hemoglobin, we found average values of 6.66 g/dL in females and 7.22 g/dL in males. The number of white blood cells was 2725 x 103/μL for females and 2775 x 103/μL for males. There was a predominance of heterophils, eosinophils, and monocytes in both sexes. No significant differences were found between males and females for hematological profile. The hematological results were compared to literature data for other Chelonia. They were similar to what is known for fresh water turtles. © FUNPEC-RP. |
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Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, BrazilHematological profileHemoglobin polymorphismPhrynops geoffroanusbufferhemoglobinacidityalkalinityanimal cellbasophilblood cell countblood samplingBrazilcontrolled studyelectrophoretic mobilityeosinophil counterythrocyte countfemalegenetic polymorphismhematocrithematological parametershemoglobin blood levelhemolysishigh performance liquid chromatographyleukocyte countlymphocyte countmalemean corpuscular volumemonocyteneutrophil countnonhumanpHsex differenceturtleurban areaAnimalsErythrocyte CountFemaleGeographyHematocritHematologic TestsHemoglobinsLeukocyte CountMalePolymorphism, GeneticTurtlesPleurodiraTestudinesComplete blood counts and hemoglobin isoform data were gathered from 36 specimens of the turtle species Phrynops geoffroanus from the northwestern region of São Paulo State, Brazil. They were collected in an urban area. The hemoglobin profiles were obtained after red blood cell lysis and by electrophoretic migration in alkaline pH, acid pH, and neutral pH buffer. The hemoglobin components were confirmed using high-performance liquid chromatography (HPLC). Erythrogram analysis included hematocrit, total hemoglobin concentration, total red blood cell count, and red blood cell indices. The leukogram included a total white blood cell count and a calculation of the percent values of neutrophils, lymphocytes, monocytes, basophils, eosinophils, heterophils, and azurophils. HPLC analysis revealed three hemoglobin components; the first with a concentration of 5.5%, the second was a major component with an average concentration of 67.1%, and the third with a concentration of 28.5%. The hematological profile obtained for these specimens allowed us to establish a pattern for P. geoffroanus in São Paulo State Northwestern region. The average hematocrit values were 22.5% for females and 24.0% for males. For total hemoglobin, we found average values of 6.66 g/dL in females and 7.22 g/dL in males. The number of white blood cells was 2725 x 103/μL for females and 2775 x 103/μL for males. There was a predominance of heterophils, eosinophils, and monocytes in both sexes. No significant differences were found between males and females for hematological profile. The hematological results were compared to literature data for other Chelonia. They were similar to what is known for fresh water turtles. © FUNPEC-RP.Departamento de Biologia, Laboratorio de Hemoglobinas e Genética das Doencas Hematologicas Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Biologia, Laboratorio de Anatomia Comparada Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Biologia, Laboratorio de Microscopia e Microanalise Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Quimica e Ciencias Ambientais, Laboratorio de Bioquimica Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Biologia, Laboratorio de Hemoglobinas e Genética das Doencas Hematologicas Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Biologia, Laboratorio de Anatomia Comparada Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Biologia, Laboratorio de Microscopia e Microanalise Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPDepartamento de Quimica e Ciencias Ambientais, Laboratorio de Bioquimica Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Zago, C. E S [UNESP]Ferrarezi, A. L. [UNESP]Vizotto, L. D. [UNESP]Oliveira, C. [UNESP]Cabral, S. R P [UNESP]Taboga, S. R. [UNESP]Bonilla-Rodriguez, G. O. [UNESP]Venancio, L. P R [UNESP]Bonini-Domingos, C. R. [UNESP]2014-05-27T11:24:44Z2014-05-27T11:24:44Z2010-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article721-726application/pdfhttp://dx.doi.org/10.4238/vol9-2gmr731Genetics and Molecular Research, v. 9, n. 2, p. 721-726, 2010.1676-5680http://hdl.handle.net/11449/7178710.4238/vol9-2gmr7312-s2.0-779544513012-s2.0-77954451301.pdf5731856650217859144525946852618832794280661767190000-0002-0970-42880000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-10-02T06:08:07Zoai:repositorio.unesp.br:11449/71787Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:48:47.083843Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
title |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
spellingShingle |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil Zago, C. E S [UNESP] Hematological profile Hemoglobin polymorphism Phrynops geoffroanus buffer hemoglobin acidity alkalinity animal cell basophil blood cell count blood sampling Brazil controlled study electrophoretic mobility eosinophil count erythrocyte count female genetic polymorphism hematocrit hematological parameters hemoglobin blood level hemolysis high performance liquid chromatography leukocyte count lymphocyte count male mean corpuscular volume monocyte neutrophil count nonhuman pH sex difference turtle urban area Animals Erythrocyte Count Female Geography Hematocrit Hematologic Tests Hemoglobins Leukocyte Count Male Polymorphism, Genetic Turtles Pleurodira Testudines |
title_short |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
title_full |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
title_fullStr |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
title_full_unstemmed |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
title_sort |
Hemoglobin polymorphism and hematological profile of Geoffroy's side-necked turtle (Phrynops geoffroanus, Testudines) in the northwestern region of São Paulo State, Brazil |
author |
Zago, C. E S [UNESP] |
author_facet |
Zago, C. E S [UNESP] Ferrarezi, A. L. [UNESP] Vizotto, L. D. [UNESP] Oliveira, C. [UNESP] Cabral, S. R P [UNESP] Taboga, S. R. [UNESP] Bonilla-Rodriguez, G. O. [UNESP] Venancio, L. P R [UNESP] Bonini-Domingos, C. R. [UNESP] |
author_role |
author |
author2 |
Ferrarezi, A. L. [UNESP] Vizotto, L. D. [UNESP] Oliveira, C. [UNESP] Cabral, S. R P [UNESP] Taboga, S. R. [UNESP] Bonilla-Rodriguez, G. O. [UNESP] Venancio, L. P R [UNESP] Bonini-Domingos, C. R. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Zago, C. E S [UNESP] Ferrarezi, A. L. [UNESP] Vizotto, L. D. [UNESP] Oliveira, C. [UNESP] Cabral, S. R P [UNESP] Taboga, S. R. [UNESP] Bonilla-Rodriguez, G. O. [UNESP] Venancio, L. P R [UNESP] Bonini-Domingos, C. R. [UNESP] |
dc.subject.por.fl_str_mv |
Hematological profile Hemoglobin polymorphism Phrynops geoffroanus buffer hemoglobin acidity alkalinity animal cell basophil blood cell count blood sampling Brazil controlled study electrophoretic mobility eosinophil count erythrocyte count female genetic polymorphism hematocrit hematological parameters hemoglobin blood level hemolysis high performance liquid chromatography leukocyte count lymphocyte count male mean corpuscular volume monocyte neutrophil count nonhuman pH sex difference turtle urban area Animals Erythrocyte Count Female Geography Hematocrit Hematologic Tests Hemoglobins Leukocyte Count Male Polymorphism, Genetic Turtles Pleurodira Testudines |
topic |
Hematological profile Hemoglobin polymorphism Phrynops geoffroanus buffer hemoglobin acidity alkalinity animal cell basophil blood cell count blood sampling Brazil controlled study electrophoretic mobility eosinophil count erythrocyte count female genetic polymorphism hematocrit hematological parameters hemoglobin blood level hemolysis high performance liquid chromatography leukocyte count lymphocyte count male mean corpuscular volume monocyte neutrophil count nonhuman pH sex difference turtle urban area Animals Erythrocyte Count Female Geography Hematocrit Hematologic Tests Hemoglobins Leukocyte Count Male Polymorphism, Genetic Turtles Pleurodira Testudines |
description |
Complete blood counts and hemoglobin isoform data were gathered from 36 specimens of the turtle species Phrynops geoffroanus from the northwestern region of São Paulo State, Brazil. They were collected in an urban area. The hemoglobin profiles were obtained after red blood cell lysis and by electrophoretic migration in alkaline pH, acid pH, and neutral pH buffer. The hemoglobin components were confirmed using high-performance liquid chromatography (HPLC). Erythrogram analysis included hematocrit, total hemoglobin concentration, total red blood cell count, and red blood cell indices. The leukogram included a total white blood cell count and a calculation of the percent values of neutrophils, lymphocytes, monocytes, basophils, eosinophils, heterophils, and azurophils. HPLC analysis revealed three hemoglobin components; the first with a concentration of 5.5%, the second was a major component with an average concentration of 67.1%, and the third with a concentration of 28.5%. The hematological profile obtained for these specimens allowed us to establish a pattern for P. geoffroanus in São Paulo State Northwestern region. The average hematocrit values were 22.5% for females and 24.0% for males. For total hemoglobin, we found average values of 6.66 g/dL in females and 7.22 g/dL in males. The number of white blood cells was 2725 x 103/μL for females and 2775 x 103/μL for males. There was a predominance of heterophils, eosinophils, and monocytes in both sexes. No significant differences were found between males and females for hematological profile. The hematological results were compared to literature data for other Chelonia. They were similar to what is known for fresh water turtles. © FUNPEC-RP. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-15 2014-05-27T11:24:44Z 2014-05-27T11:24:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol9-2gmr731 Genetics and Molecular Research, v. 9, n. 2, p. 721-726, 2010. 1676-5680 http://hdl.handle.net/11449/71787 10.4238/vol9-2gmr731 2-s2.0-77954451301 2-s2.0-77954451301.pdf 5731856650217859 1445259468526188 3279428066176719 0000-0002-0970-4288 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.4238/vol9-2gmr731 http://hdl.handle.net/11449/71787 |
identifier_str_mv |
Genetics and Molecular Research, v. 9, n. 2, p. 721-726, 2010. 1676-5680 10.4238/vol9-2gmr731 2-s2.0-77954451301 2-s2.0-77954451301.pdf 5731856650217859 1445259468526188 3279428066176719 0000-0002-0970-4288 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
721-726 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128278490251264 |