Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches

Detalhes bibliográficos
Autor(a) principal: Senhorello, Igor L. S. [UNESP]
Data de Publicação: 2020
Outros Autores: Terra, Erika M., Sueiro, Felipe A. R., Firmo, Bruna F. [UNESP], Anai, Leticia A. [UNESP], Goloni, Camila [UNESP], Canavari, Isabela C. [UNESP], Ampuero, Roberto A. N. [UNESP], Pereira, Rodrigo S., Tinucci-Costa, Mirela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/vco.12552
http://hdl.handle.net/11449/198151
Resumo: This study aimed at evaluating the behaviour and understanding the diagnostic value of the carcinoembryonic antigen (CEA) in bitches with mammary carcinoma as a tool for monitoring and prognosis of canine cancer patients. Serum samples from 77 bitches were divided into four groups, G1 (n = 21), control group (healthy/neoplasia free bitches); G2 (n = 31), bitches with non-metastatic mammary carcinoma less than 3 cm; G3 (n = 12), bitches with non-metastatic mammary carcinoma greater than 3 cm; and, G4 (n = 13) bitches with mammary carcinoma and lymph node metastasis. The marker was dosed once in G1, whereas in G2, G3 and G4, CEA levels were determined before (M0) and 15 days after (M1) mastectomy, using the ELISA kit for humans while reading used ELISYS ONE human. A group of 11 bitches was followed up 45 days after mastectomy (M2). The results for the concentration of markers in blood serum samples at the evaluated times and their relationship with neoplasia biological behaviour and observed clinicopathological changes were evaluated by the Tukey test at 5% significance. The ROC curve was established to find the cut-off value and calculate the test sensitivity and specificity, the multivariate matching analysis was performed to confirm the association between CEA values and clinicopathological variables. CEA values increased significantly in bitches with mammary carcinoma, metastatic tumours with a diameter larger than 3.0 cm and high grade, compared with healthy ones. In addition, mastectomy reduced the CEA concentration in the blood (P <.05) whereas high CEA levels were associated with unfavourable prognostic factors (P <.05). The biomarker presented good diagnostic value, especially for more aggressive tumours. In conclusion, CEA serum concentrations allowed to follow efficiently the evolution of mammary tumours in bitches, since CEA values increased in bitches with mammary gland tumour and decreased after mastectomy while correlating with prognostic factors such as tumour size, nodal metastasis and histological grade. Further studies are still needed to confirm its diagnostic value for follow-up of relapse and early metastasis.
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spelling Clinical value of carcinoembryonic antigen in mammary neoplasms of bitchesbiomarkerscanine mammary tumourCEAmetastasisprognosisThis study aimed at evaluating the behaviour and understanding the diagnostic value of the carcinoembryonic antigen (CEA) in bitches with mammary carcinoma as a tool for monitoring and prognosis of canine cancer patients. Serum samples from 77 bitches were divided into four groups, G1 (n = 21), control group (healthy/neoplasia free bitches); G2 (n = 31), bitches with non-metastatic mammary carcinoma less than 3 cm; G3 (n = 12), bitches with non-metastatic mammary carcinoma greater than 3 cm; and, G4 (n = 13) bitches with mammary carcinoma and lymph node metastasis. The marker was dosed once in G1, whereas in G2, G3 and G4, CEA levels were determined before (M0) and 15 days after (M1) mastectomy, using the ELISA kit for humans while reading used ELISYS ONE human. A group of 11 bitches was followed up 45 days after mastectomy (M2). The results for the concentration of markers in blood serum samples at the evaluated times and their relationship with neoplasia biological behaviour and observed clinicopathological changes were evaluated by the Tukey test at 5% significance. The ROC curve was established to find the cut-off value and calculate the test sensitivity and specificity, the multivariate matching analysis was performed to confirm the association between CEA values and clinicopathological variables. CEA values increased significantly in bitches with mammary carcinoma, metastatic tumours with a diameter larger than 3.0 cm and high grade, compared with healthy ones. In addition, mastectomy reduced the CEA concentration in the blood (P <.05) whereas high CEA levels were associated with unfavourable prognostic factors (P <.05). The biomarker presented good diagnostic value, especially for more aggressive tumours. In conclusion, CEA serum concentrations allowed to follow efficiently the evolution of mammary tumours in bitches, since CEA values increased in bitches with mammary gland tumour and decreased after mastectomy while correlating with prognostic factors such as tumour size, nodal metastasis and histological grade. Further studies are still needed to confirm its diagnostic value for follow-up of relapse and early metastasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences UNESPVeterinarian Nestlé Purina PetCareVeterinarian VETPAT Pathology and Molecular Biology LaboratoryDepartment of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences UNESPFAPESP: 2015/15015-9FAPESP: 2016/00128-5Universidade Estadual Paulista (Unesp)Nestlé Purina PetCareVETPAT Pathology and Molecular Biology LaboratorySenhorello, Igor L. S. [UNESP]Terra, Erika M.Sueiro, Felipe A. R.Firmo, Bruna F. [UNESP]Anai, Leticia A. [UNESP]Goloni, Camila [UNESP]Canavari, Isabela C. [UNESP]Ampuero, Roberto A. N. [UNESP]Pereira, Rodrigo S.Tinucci-Costa, Mirela [UNESP]2020-12-12T01:00:34Z2020-12-12T01:00:34Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article315-323http://dx.doi.org/10.1111/vco.12552Veterinary and Comparative Oncology, v. 18, n. 3, p. 315-323, 2020.1476-58291476-5810http://hdl.handle.net/11449/19815110.1111/vco.125522-s2.0-85075127526Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary and Comparative Oncologyinfo:eu-repo/semantics/openAccess2021-10-23T09:13:45Zoai:repositorio.unesp.br:11449/198151Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:10.353921Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
title Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
spellingShingle Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
Senhorello, Igor L. S. [UNESP]
biomarkers
canine mammary tumour
CEA
metastasis
prognosis
title_short Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
title_full Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
title_fullStr Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
title_full_unstemmed Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
title_sort Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches
author Senhorello, Igor L. S. [UNESP]
author_facet Senhorello, Igor L. S. [UNESP]
Terra, Erika M.
Sueiro, Felipe A. R.
Firmo, Bruna F. [UNESP]
Anai, Leticia A. [UNESP]
Goloni, Camila [UNESP]
Canavari, Isabela C. [UNESP]
Ampuero, Roberto A. N. [UNESP]
Pereira, Rodrigo S.
Tinucci-Costa, Mirela [UNESP]
author_role author
author2 Terra, Erika M.
Sueiro, Felipe A. R.
Firmo, Bruna F. [UNESP]
Anai, Leticia A. [UNESP]
Goloni, Camila [UNESP]
Canavari, Isabela C. [UNESP]
Ampuero, Roberto A. N. [UNESP]
Pereira, Rodrigo S.
Tinucci-Costa, Mirela [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Nestlé Purina PetCare
VETPAT Pathology and Molecular Biology Laboratory
dc.contributor.author.fl_str_mv Senhorello, Igor L. S. [UNESP]
Terra, Erika M.
Sueiro, Felipe A. R.
Firmo, Bruna F. [UNESP]
Anai, Leticia A. [UNESP]
Goloni, Camila [UNESP]
Canavari, Isabela C. [UNESP]
Ampuero, Roberto A. N. [UNESP]
Pereira, Rodrigo S.
Tinucci-Costa, Mirela [UNESP]
dc.subject.por.fl_str_mv biomarkers
canine mammary tumour
CEA
metastasis
prognosis
topic biomarkers
canine mammary tumour
CEA
metastasis
prognosis
description This study aimed at evaluating the behaviour and understanding the diagnostic value of the carcinoembryonic antigen (CEA) in bitches with mammary carcinoma as a tool for monitoring and prognosis of canine cancer patients. Serum samples from 77 bitches were divided into four groups, G1 (n = 21), control group (healthy/neoplasia free bitches); G2 (n = 31), bitches with non-metastatic mammary carcinoma less than 3 cm; G3 (n = 12), bitches with non-metastatic mammary carcinoma greater than 3 cm; and, G4 (n = 13) bitches with mammary carcinoma and lymph node metastasis. The marker was dosed once in G1, whereas in G2, G3 and G4, CEA levels were determined before (M0) and 15 days after (M1) mastectomy, using the ELISA kit for humans while reading used ELISYS ONE human. A group of 11 bitches was followed up 45 days after mastectomy (M2). The results for the concentration of markers in blood serum samples at the evaluated times and their relationship with neoplasia biological behaviour and observed clinicopathological changes were evaluated by the Tukey test at 5% significance. The ROC curve was established to find the cut-off value and calculate the test sensitivity and specificity, the multivariate matching analysis was performed to confirm the association between CEA values and clinicopathological variables. CEA values increased significantly in bitches with mammary carcinoma, metastatic tumours with a diameter larger than 3.0 cm and high grade, compared with healthy ones. In addition, mastectomy reduced the CEA concentration in the blood (P <.05) whereas high CEA levels were associated with unfavourable prognostic factors (P <.05). The biomarker presented good diagnostic value, especially for more aggressive tumours. In conclusion, CEA serum concentrations allowed to follow efficiently the evolution of mammary tumours in bitches, since CEA values increased in bitches with mammary gland tumour and decreased after mastectomy while correlating with prognostic factors such as tumour size, nodal metastasis and histological grade. Further studies are still needed to confirm its diagnostic value for follow-up of relapse and early metastasis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:00:34Z
2020-12-12T01:00:34Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/vco.12552
Veterinary and Comparative Oncology, v. 18, n. 3, p. 315-323, 2020.
1476-5829
1476-5810
http://hdl.handle.net/11449/198151
10.1111/vco.12552
2-s2.0-85075127526
url http://dx.doi.org/10.1111/vco.12552
http://hdl.handle.net/11449/198151
identifier_str_mv Veterinary and Comparative Oncology, v. 18, n. 3, p. 315-323, 2020.
1476-5829
1476-5810
10.1111/vco.12552
2-s2.0-85075127526
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary and Comparative Oncology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 315-323
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129532757016576