MALDI-TOF MS identification of Prototheca algae associated with bovine mastitis

• Autor(a) principal: Fidelis, Carlos E.
• Data de Publicação: 2021
• Outros Autores: Franke, Manoela, de Abreu, Letícia C. R., Jagielski, Tomasz, Ribeiro, Márcio G. [UNESP], dos Santos, Marcos V., Gonçalves, Juliano L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1177/10406387211036459; http://hdl.handle.net/11449/229296
• topic: Brazil; mass spectrometry; microalgae; Prototheca

We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases.