Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/ptr.7618 http://hdl.handle.net/11449/242259 |
Resumo: | Different propolis samples can be obtained in Brazil, such as green, brown and red. Studies related to Brazilian red propolis (BRP) have increased in the last few years, so the aim of this study was to investigate its effects on the prostate cell lines LNCaP and PC-3 and on human monocytes. BRP chemical composition was analyzed by HPLC-DAD, the viability of monocyte and cancer cell by MTT assay. Cytokine production (TNF-α, IL-1β, IL-6, IL-10) by monocytes was quantitated by ELISA, the expression of cell markers (TLR-2, TLR-4, HLA-DR, CD80) and reactive oxygen species by flow cytometry. The candidacidal activity and the effects of supernatant of treated monocytes on tumor cells were assessed. BRP affected LNCaP viability after 48 and 72 h, while PC-3 cells were more resistant over time. BRP upregulated CD80 and HLA-DR expression, and stimulated TNF-α, IL-6 and IL-10 production. BRP enhanced the fungicidal activity of monocytes, displayed an antioxidant action and the supernatant of BRP-treated monocytes diminished LNCaP viability. In the search for new immunomodulatory and antitumoral agents, BRP exerted a selective cytotoxic activity on prostate cancer cells and an immunomodulatory action, suggesting its potential for clinical trials with oncological patients and for the discovery of new immunomodulatory and antitumor drugs. |
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Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functionscytokineimmunomodulationmonocytepropolisprostate cancertoll-like receptorDifferent propolis samples can be obtained in Brazil, such as green, brown and red. Studies related to Brazilian red propolis (BRP) have increased in the last few years, so the aim of this study was to investigate its effects on the prostate cell lines LNCaP and PC-3 and on human monocytes. BRP chemical composition was analyzed by HPLC-DAD, the viability of monocyte and cancer cell by MTT assay. Cytokine production (TNF-α, IL-1β, IL-6, IL-10) by monocytes was quantitated by ELISA, the expression of cell markers (TLR-2, TLR-4, HLA-DR, CD80) and reactive oxygen species by flow cytometry. The candidacidal activity and the effects of supernatant of treated monocytes on tumor cells were assessed. BRP affected LNCaP viability after 48 and 72 h, while PC-3 cells were more resistant over time. BRP upregulated CD80 and HLA-DR expression, and stimulated TNF-α, IL-6 and IL-10 production. BRP enhanced the fungicidal activity of monocytes, displayed an antioxidant action and the supernatant of BRP-treated monocytes diminished LNCaP viability. In the search for new immunomodulatory and antitumoral agents, BRP exerted a selective cytotoxic activity on prostate cancer cells and an immunomodulatory action, suggesting its potential for clinical trials with oncological patients and for the discovery of new immunomodulatory and antitumor drugs.São Paulo State University (UNESP) Institute of BiosciencesDepartment of Health Science Oeste Paulista University (UNOESTE)School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP)São Paulo State University (UNESP) Institute of BiosciencesUniversidade Estadual Paulista (UNESP)Oeste Paulista University (UNOESTE)Universidade de São Paulo (USP)Santiago, Karina Basso [UNESP]Rodrigues, João Carlos Zamae [UNESP]de Oliveira Cardoso, Eliza [UNESP]Conte, Fernanda Lopes [UNESP]Tasca, Karen Ingrid [UNESP]Romagnoli, Graziela GoreteAldana-Mejía, Jennyfer AndreaBastos, Jairo KenuppSforcin, José Maurício [UNESP]2023-03-02T12:26:06Z2023-03-02T12:26:06Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/ptr.7618Phytotherapy Research.1099-15730951-418Xhttp://hdl.handle.net/11449/24225910.1002/ptr.76182-s2.0-85137576016Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytotherapy Researchinfo:eu-repo/semantics/openAccess2023-03-02T12:26:06Zoai:repositorio.unesp.br:11449/242259Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T12:26:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
title |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
spellingShingle |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions Santiago, Karina Basso [UNESP] cytokine immunomodulation monocyte propolis prostate cancer toll-like receptor |
title_short |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
title_full |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
title_fullStr |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
title_full_unstemmed |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
title_sort |
Brazilian red propolis exerts a cytotoxic action against prostate cancer cells and upregulates human monocyte functions |
author |
Santiago, Karina Basso [UNESP] |
author_facet |
Santiago, Karina Basso [UNESP] Rodrigues, João Carlos Zamae [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Tasca, Karen Ingrid [UNESP] Romagnoli, Graziela Gorete Aldana-Mejía, Jennyfer Andrea Bastos, Jairo Kenupp Sforcin, José Maurício [UNESP] |
author_role |
author |
author2 |
Rodrigues, João Carlos Zamae [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Tasca, Karen Ingrid [UNESP] Romagnoli, Graziela Gorete Aldana-Mejía, Jennyfer Andrea Bastos, Jairo Kenupp Sforcin, José Maurício [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Oeste Paulista University (UNOESTE) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Santiago, Karina Basso [UNESP] Rodrigues, João Carlos Zamae [UNESP] de Oliveira Cardoso, Eliza [UNESP] Conte, Fernanda Lopes [UNESP] Tasca, Karen Ingrid [UNESP] Romagnoli, Graziela Gorete Aldana-Mejía, Jennyfer Andrea Bastos, Jairo Kenupp Sforcin, José Maurício [UNESP] |
dc.subject.por.fl_str_mv |
cytokine immunomodulation monocyte propolis prostate cancer toll-like receptor |
topic |
cytokine immunomodulation monocyte propolis prostate cancer toll-like receptor |
description |
Different propolis samples can be obtained in Brazil, such as green, brown and red. Studies related to Brazilian red propolis (BRP) have increased in the last few years, so the aim of this study was to investigate its effects on the prostate cell lines LNCaP and PC-3 and on human monocytes. BRP chemical composition was analyzed by HPLC-DAD, the viability of monocyte and cancer cell by MTT assay. Cytokine production (TNF-α, IL-1β, IL-6, IL-10) by monocytes was quantitated by ELISA, the expression of cell markers (TLR-2, TLR-4, HLA-DR, CD80) and reactive oxygen species by flow cytometry. The candidacidal activity and the effects of supernatant of treated monocytes on tumor cells were assessed. BRP affected LNCaP viability after 48 and 72 h, while PC-3 cells were more resistant over time. BRP upregulated CD80 and HLA-DR expression, and stimulated TNF-α, IL-6 and IL-10 production. BRP enhanced the fungicidal activity of monocytes, displayed an antioxidant action and the supernatant of BRP-treated monocytes diminished LNCaP viability. In the search for new immunomodulatory and antitumoral agents, BRP exerted a selective cytotoxic activity on prostate cancer cells and an immunomodulatory action, suggesting its potential for clinical trials with oncological patients and for the discovery of new immunomodulatory and antitumor drugs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T12:26:06Z 2023-03-02T12:26:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ptr.7618 Phytotherapy Research. 1099-1573 0951-418X http://hdl.handle.net/11449/242259 10.1002/ptr.7618 2-s2.0-85137576016 |
url |
http://dx.doi.org/10.1002/ptr.7618 http://hdl.handle.net/11449/242259 |
identifier_str_mv |
Phytotherapy Research. 1099-1573 0951-418X 10.1002/ptr.7618 2-s2.0-85137576016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytotherapy Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964630015016960 |