Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy

Detalhes bibliográficos
Autor(a) principal: Daré, Regina G.
Data de Publicação: 2020
Outros Autores: Costa, Ana, Nakamura, Celso V., Truiti, Maria C.T., Ximenes, Valdecir F. [UNESP], Lautenschlager, Sueli O.S., Sarmento, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ijpharm.2020.119336
http://hdl.handle.net/11449/198734
Resumo: Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery.
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spelling Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategyEthyl protocatechuateFranz cellsNanostructured lipid carriersProtocatechuic acidSolid lipid nanoparticlesTopical deliveryExcessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Pharmacy State University of Maringá (UEM)i3S – Institute for Research and Innovation in Health University of Porto, Rua Alfredo Allen, 208INEB – Institute of Biomedical Engineering University of Porto, Rua Alfredo Allen, 208Department of Chemistry Faculty of Sciences University of São Paulo (UNESP)CESPU IINFACTS – Institute for Research and Advanced Training in Health Sciences and Technologies, Rua Central de Gandra 1317Department of Chemistry Faculty of Sciences University of São Paulo (UNESP)FAPESP: 2014/50926-0CAPES: 88881.188573/2018-01Universidade Estadual de Maringá (UEM)University of PortoUniversidade Estadual Paulista (Unesp)IINFACTS – Institute for Research and Advanced Training in Health Sciences and TechnologiesDaré, Regina G.Costa, AnaNakamura, Celso V.Truiti, Maria C.T.Ximenes, Valdecir F. [UNESP]Lautenschlager, Sueli O.S.Sarmento, Bruno2020-12-12T01:20:42Z2020-12-12T01:20:42Z2020-05-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ijpharm.2020.119336International Journal of Pharmaceutics, v. 582.1873-34760378-5173http://hdl.handle.net/11449/19873410.1016/j.ijpharm.2020.1193362-s2.0-85083295842Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Pharmaceuticsinfo:eu-repo/semantics/openAccess2021-10-22T20:04:12Zoai:repositorio.unesp.br:11449/198734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:53:44.083956Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
title Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
spellingShingle Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
Daré, Regina G.
Ethyl protocatechuate
Franz cells
Nanostructured lipid carriers
Protocatechuic acid
Solid lipid nanoparticles
Topical delivery
title_short Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
title_full Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
title_fullStr Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
title_full_unstemmed Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
title_sort Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
author Daré, Regina G.
author_facet Daré, Regina G.
Costa, Ana
Nakamura, Celso V.
Truiti, Maria C.T.
Ximenes, Valdecir F. [UNESP]
Lautenschlager, Sueli O.S.
Sarmento, Bruno
author_role author
author2 Costa, Ana
Nakamura, Celso V.
Truiti, Maria C.T.
Ximenes, Valdecir F. [UNESP]
Lautenschlager, Sueli O.S.
Sarmento, Bruno
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
University of Porto
Universidade Estadual Paulista (Unesp)
IINFACTS – Institute for Research and Advanced Training in Health Sciences and Technologies
dc.contributor.author.fl_str_mv Daré, Regina G.
Costa, Ana
Nakamura, Celso V.
Truiti, Maria C.T.
Ximenes, Valdecir F. [UNESP]
Lautenschlager, Sueli O.S.
Sarmento, Bruno
dc.subject.por.fl_str_mv Ethyl protocatechuate
Franz cells
Nanostructured lipid carriers
Protocatechuic acid
Solid lipid nanoparticles
Topical delivery
topic Ethyl protocatechuate
Franz cells
Nanostructured lipid carriers
Protocatechuic acid
Solid lipid nanoparticles
Topical delivery
description Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:20:42Z
2020-12-12T01:20:42Z
2020-05-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ijpharm.2020.119336
International Journal of Pharmaceutics, v. 582.
1873-3476
0378-5173
http://hdl.handle.net/11449/198734
10.1016/j.ijpharm.2020.119336
2-s2.0-85083295842
url http://dx.doi.org/10.1016/j.ijpharm.2020.119336
http://hdl.handle.net/11449/198734
identifier_str_mv International Journal of Pharmaceutics, v. 582.
1873-3476
0378-5173
10.1016/j.ijpharm.2020.119336
2-s2.0-85083295842
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Pharmaceutics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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