Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijpharm.2020.119336 http://hdl.handle.net/11449/198734 |
Resumo: | Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery. |
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Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategyEthyl protocatechuateFranz cellsNanostructured lipid carriersProtocatechuic acidSolid lipid nanoparticlesTopical deliveryExcessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Pharmacy State University of Maringá (UEM)i3S – Institute for Research and Innovation in Health University of Porto, Rua Alfredo Allen, 208INEB – Institute of Biomedical Engineering University of Porto, Rua Alfredo Allen, 208Department of Chemistry Faculty of Sciences University of São Paulo (UNESP)CESPU IINFACTS – Institute for Research and Advanced Training in Health Sciences and Technologies, Rua Central de Gandra 1317Department of Chemistry Faculty of Sciences University of São Paulo (UNESP)FAPESP: 2014/50926-0CAPES: 88881.188573/2018-01Universidade Estadual de Maringá (UEM)University of PortoUniversidade Estadual Paulista (Unesp)IINFACTS – Institute for Research and Advanced Training in Health Sciences and TechnologiesDaré, Regina G.Costa, AnaNakamura, Celso V.Truiti, Maria C.T.Ximenes, Valdecir F. [UNESP]Lautenschlager, Sueli O.S.Sarmento, Bruno2020-12-12T01:20:42Z2020-12-12T01:20:42Z2020-05-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ijpharm.2020.119336International Journal of Pharmaceutics, v. 582.1873-34760378-5173http://hdl.handle.net/11449/19873410.1016/j.ijpharm.2020.1193362-s2.0-85083295842Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Pharmaceuticsinfo:eu-repo/semantics/openAccess2021-10-22T20:04:12Zoai:repositorio.unesp.br:11449/198734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:53:44.083956Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
title |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
spellingShingle |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy Daré, Regina G. Ethyl protocatechuate Franz cells Nanostructured lipid carriers Protocatechuic acid Solid lipid nanoparticles Topical delivery |
title_short |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
title_full |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
title_fullStr |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
title_full_unstemmed |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
title_sort |
Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy |
author |
Daré, Regina G. |
author_facet |
Daré, Regina G. Costa, Ana Nakamura, Celso V. Truiti, Maria C.T. Ximenes, Valdecir F. [UNESP] Lautenschlager, Sueli O.S. Sarmento, Bruno |
author_role |
author |
author2 |
Costa, Ana Nakamura, Celso V. Truiti, Maria C.T. Ximenes, Valdecir F. [UNESP] Lautenschlager, Sueli O.S. Sarmento, Bruno |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) University of Porto Universidade Estadual Paulista (Unesp) IINFACTS – Institute for Research and Advanced Training in Health Sciences and Technologies |
dc.contributor.author.fl_str_mv |
Daré, Regina G. Costa, Ana Nakamura, Celso V. Truiti, Maria C.T. Ximenes, Valdecir F. [UNESP] Lautenschlager, Sueli O.S. Sarmento, Bruno |
dc.subject.por.fl_str_mv |
Ethyl protocatechuate Franz cells Nanostructured lipid carriers Protocatechuic acid Solid lipid nanoparticles Topical delivery |
topic |
Ethyl protocatechuate Franz cells Nanostructured lipid carriers Protocatechuic acid Solid lipid nanoparticles Topical delivery |
description |
Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, −2.2 to −5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4○C and 25○C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:20:42Z 2020-12-12T01:20:42Z 2020-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijpharm.2020.119336 International Journal of Pharmaceutics, v. 582. 1873-3476 0378-5173 http://hdl.handle.net/11449/198734 10.1016/j.ijpharm.2020.119336 2-s2.0-85083295842 |
url |
http://dx.doi.org/10.1016/j.ijpharm.2020.119336 http://hdl.handle.net/11449/198734 |
identifier_str_mv |
International Journal of Pharmaceutics, v. 582. 1873-3476 0378-5173 10.1016/j.ijpharm.2020.119336 2-s2.0-85083295842 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Pharmaceutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129471031541760 |