Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128590 |
Resumo: | This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraiso Pedres (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line. All of the analyzed loci were informative (PIC>0.5). These results indicate that these free-range animals have a high genetic variability, at least for the majority of the analyzed loci, and this genetic variation is higher than the commercial chickens and similar for the no-commercial birds. |
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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines(Caipira) broiler line(Caipira) layer lineMicrosatellitePolymorphismThis study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraiso Pedres (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line. All of the analyzed loci were informative (PIC>0.5). These results indicate that these free-range animals have a high genetic variability, at least for the majority of the analyzed loci, and this genetic variation is higher than the commercial chickens and similar for the no-commercial birds.Univ Estado Santa Catarina UDESC, Ctr Ciencias Agrovet CAV, DNA UDESC, Lab Anal Genet, Lages, SC, BrazilUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Genet, Botucatu, SP, BrazilUniv Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Lab Biotecnol, Piracicaba, SP, BrazilUDESC, Ctr Educ Super Regiao Sul CERES, Lab Genet Mol LAGENMOL, BR-88790000 Laguna, SC, BrazilUniversidade Estadual Paulista, UNESP, Inst Biociencias, Dept Genet, Botucatu, SP, BrazilUniv Federal Santa MariaUniversidade de Estado de Santa Catarina (UDESC)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Pagani Possamai, Mari HelenBattilana, JaquelinePaludo, EdianeHerkenhoff, Marcos Edgar [UNESP]Pertille, FabioLima-Rosa, Carlos Andre da Veiga2015-10-21T13:11:17Z2015-10-21T13:11:17Z2015-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article877-883application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877&lng=en&nrm=iso&tlng=enCiencia Rural, v. 45, n. 5, p. 877-883, 2015.0103-8478http://hdl.handle.net/11449/12859010.1590/0103-8478cr20140132S0103-84782015000500877WOS:000354789100021S0103-84782015000500877.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0.5250,337info:eu-repo/semantics/openAccess2023-10-08T06:07:27Zoai:repositorio.unesp.br:11449/128590Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:21:18.118062Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
title |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
spellingShingle |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines Pagani Possamai, Mari Helen (Caipira) broiler line (Caipira) layer line Microsatellite Polymorphism |
title_short |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
title_full |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
title_fullStr |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
title_full_unstemmed |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
title_sort |
Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines |
author |
Pagani Possamai, Mari Helen |
author_facet |
Pagani Possamai, Mari Helen Battilana, Jaqueline Paludo, Ediane Herkenhoff, Marcos Edgar [UNESP] Pertille, Fabio Lima-Rosa, Carlos Andre da Veiga |
author_role |
author |
author2 |
Battilana, Jaqueline Paludo, Ediane Herkenhoff, Marcos Edgar [UNESP] Pertille, Fabio Lima-Rosa, Carlos Andre da Veiga |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Estado de Santa Catarina (UDESC) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pagani Possamai, Mari Helen Battilana, Jaqueline Paludo, Ediane Herkenhoff, Marcos Edgar [UNESP] Pertille, Fabio Lima-Rosa, Carlos Andre da Veiga |
dc.subject.por.fl_str_mv |
(Caipira) broiler line (Caipira) layer line Microsatellite Polymorphism |
topic |
(Caipira) broiler line (Caipira) layer line Microsatellite Polymorphism |
description |
This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraiso Pedres (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line. All of the analyzed loci were informative (PIC>0.5). These results indicate that these free-range animals have a high genetic variability, at least for the majority of the analyzed loci, and this genetic variation is higher than the commercial chickens and similar for the no-commercial birds. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:11:17Z 2015-10-21T13:11:17Z 2015-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877&lng=en&nrm=iso&tlng=en Ciencia Rural, v. 45, n. 5, p. 877-883, 2015. 0103-8478 http://hdl.handle.net/11449/128590 10.1590/0103-8478cr20140132 S0103-84782015000500877 WOS:000354789100021 S0103-84782015000500877.pdf |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128590 |
identifier_str_mv |
Ciencia Rural, v. 45, n. 5, p. 877-883, 2015. 0103-8478 10.1590/0103-8478cr20140132 S0103-84782015000500877 WOS:000354789100021 S0103-84782015000500877.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Rural 0.525 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
877-883 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Federal Santa Maria |
publisher.none.fl_str_mv |
Univ Federal Santa Maria |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128350882889728 |