Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.15376/biores.10.2.3197-3209 http://hdl.handle.net/11449/228039 |
Resumo: | This study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 °C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1:1 to 1:3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum. |
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Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat strawGanoderma lucidumLignocellulolytic enzymesSolid state fermentationThis study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 °C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1:1 to 1:3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum.Módulo de Cogumelos, Departamento de Defesa Fitossanitária, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, 18603-970, P.O. Box 237, Botucatu, São PauloCentre for Biocomposites and Biomaterials Processing, Faculty of Forestry, University of TorontoCenter of Excellence for Advanced Materials Research, King AbdulAziz UniversityCentro de Energia Nuclear na Agricultura, University of São Paulo, P.O. Box 96Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, P.O. Box. 237Universidade do Sagrado Coração, Centro de Ciências Exatas e Sociais Aplicadas, Rua Irmã Arminda, 10-50, Jardim BrasilMódulo de Cogumelos, Departamento de Defesa Fitossanitária, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, 18603-970, P.O. Box 237, Botucatu, São PauloDepartamento de Engenharia Rural, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, P.O. Box. 237Universidade Estadual Paulista (UNESP)Centre for Biocomposites and Biomaterials Processing, Faculty of Forestry, University of TorontoCenter of Excellence for Advanced Materials Research, King AbdulAziz UniversityUniversidade de São Paulo (USP)Universidade do Sagrado Coração, Centro de Ciências Exatas e Sociais AplicadasFurlan de Jesus, João Paulo [UNESP]Sain, MohiniJeng, RobertNegrão, Djanira RodriguesLeão, Alcides Lopes [UNESP]Nogueira de Andrade, Meire CristinaAlmeida Minhoni, Marli Teixeira de [UNESP]2022-04-29T07:26:26Z2022-04-29T07:26:26Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3197-3209http://dx.doi.org/10.15376/biores.10.2.3197-3209BioResources, v. 10, n. 2, p. 3197-3209, 2015.1930-2126http://hdl.handle.net/11449/22803910.15376/biores.10.2.3197-32092-s2.0-84940883814Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioResourcesinfo:eu-repo/semantics/openAccess2024-04-30T18:07:20Zoai:repositorio.unesp.br:11449/228039Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T18:07:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
spellingShingle |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw Furlan de Jesus, João Paulo [UNESP] Ganoderma lucidum Lignocellulolytic enzymes Solid state fermentation |
title_short |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_full |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_fullStr |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_full_unstemmed |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_sort |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
author |
Furlan de Jesus, João Paulo [UNESP] |
author_facet |
Furlan de Jesus, João Paulo [UNESP] Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Nogueira de Andrade, Meire Cristina Almeida Minhoni, Marli Teixeira de [UNESP] |
author_role |
author |
author2 |
Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Nogueira de Andrade, Meire Cristina Almeida Minhoni, Marli Teixeira de [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Centre for Biocomposites and Biomaterials Processing, Faculty of Forestry, University of Toronto Center of Excellence for Advanced Materials Research, King AbdulAziz University Universidade de São Paulo (USP) Universidade do Sagrado Coração, Centro de Ciências Exatas e Sociais Aplicadas |
dc.contributor.author.fl_str_mv |
Furlan de Jesus, João Paulo [UNESP] Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Nogueira de Andrade, Meire Cristina Almeida Minhoni, Marli Teixeira de [UNESP] |
dc.subject.por.fl_str_mv |
Ganoderma lucidum Lignocellulolytic enzymes Solid state fermentation |
topic |
Ganoderma lucidum Lignocellulolytic enzymes Solid state fermentation |
description |
This study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 °C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1:1 to 1:3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2022-04-29T07:26:26Z 2022-04-29T07:26:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.15376/biores.10.2.3197-3209 BioResources, v. 10, n. 2, p. 3197-3209, 2015. 1930-2126 http://hdl.handle.net/11449/228039 10.15376/biores.10.2.3197-3209 2-s2.0-84940883814 |
url |
http://dx.doi.org/10.15376/biores.10.2.3197-3209 http://hdl.handle.net/11449/228039 |
identifier_str_mv |
BioResources, v. 10, n. 2, p. 3197-3209, 2015. 1930-2126 10.15376/biores.10.2.3197-3209 2-s2.0-84940883814 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BioResources |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3197-3209 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965060712366080 |