Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_3197_Furlan_deJesus_Application%20of%20Ganoderma_lucidum http://hdl.handle.net/11449/129277 |
Resumo: | This study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 degrees C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1: 1 to 1: 3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum. |
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Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat strawGanoderma lucidumSolid state fermentationLignocellulolytic enzymesThis study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 degrees C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1: 1 to 1: 3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Foreign Affairs and International Trade Canada (DFAIT)Canadian Bureau for International Education (CBIE)University of Toronto, Centre for Biocomposites and Biomaterials Processing, Faculty of ForestryKing AbdulAziz University, Center of Excellence for Advanced Materials ResearchUniversidade de São Paulo, Centro de Energia Nuclear na Agricultura de PiracicabaUniversidade do Sagrado Coração, Centro de Ciências Exatas e Sociais AplicadasUniversidade Estadual Paulista, Departamento de Proteção Vegetal, Faculdade de Ciências Agronômicas de BotucatuNorth Carolina State Univ Dept Wood &paper SciUniversidade Estadual Paulista (Unesp)University of TorontoKing Abdulaziz UniversityUniversidade de São Paulo (USP)Universidade do Sagrado CoraçãoJesus, João Paulo Furlan de [UNESP]Sain, MohiniJeng, RobertNegrão, Djanira RodriguesLeão, Alcides Lopes [UNESP]Andrade, Meire Cristina Nogueira deMinhoni, Marli Teixeira de Almeida [UNESP]2015-10-21T20:44:40Z2015-10-21T20:44:40Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3197-3209application/pdfhttp://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_3197_Furlan_deJesus_Application%20of%20Ganoderma_lucidumBioresources. Raleigh: North Carolina State Univ Dept Wood &paper Sci, v. 10, n. 2, p. 3197-3209, 2015.1930-2126http://hdl.handle.net/11449/129277WOS:000354642000098WOS000354642000098.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioresources1.2020,405info:eu-repo/semantics/openAccess2024-04-30T18:07:43Zoai:repositorio.unesp.br:11449/129277Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T18:07:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
spellingShingle |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw Jesus, João Paulo Furlan de [UNESP] Ganoderma lucidum Solid state fermentation Lignocellulolytic enzymes |
title_short |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_full |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_fullStr |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_full_unstemmed |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
title_sort |
Potential application of Ganoderma lucidum in solid state fermentation of primary sludge and wheat straw |
author |
Jesus, João Paulo Furlan de [UNESP] |
author_facet |
Jesus, João Paulo Furlan de [UNESP] Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Andrade, Meire Cristina Nogueira de Minhoni, Marli Teixeira de Almeida [UNESP] |
author_role |
author |
author2 |
Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Andrade, Meire Cristina Nogueira de Minhoni, Marli Teixeira de Almeida [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Toronto King Abdulaziz University Universidade de São Paulo (USP) Universidade do Sagrado Coração |
dc.contributor.author.fl_str_mv |
Jesus, João Paulo Furlan de [UNESP] Sain, Mohini Jeng, Robert Negrão, Djanira Rodrigues Leão, Alcides Lopes [UNESP] Andrade, Meire Cristina Nogueira de Minhoni, Marli Teixeira de Almeida [UNESP] |
dc.subject.por.fl_str_mv |
Ganoderma lucidum Solid state fermentation Lignocellulolytic enzymes |
topic |
Ganoderma lucidum Solid state fermentation Lignocellulolytic enzymes |
description |
This study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 degrees C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1: 1 to 1: 3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T20:44:40Z 2015-10-21T20:44:40Z 2015-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_3197_Furlan_deJesus_Application%20of%20Ganoderma_lucidum Bioresources. Raleigh: North Carolina State Univ Dept Wood &paper Sci, v. 10, n. 2, p. 3197-3209, 2015. 1930-2126 http://hdl.handle.net/11449/129277 WOS:000354642000098 WOS000354642000098.pdf |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_2_3197_Furlan_deJesus_Application%20of%20Ganoderma_lucidum http://hdl.handle.net/11449/129277 |
identifier_str_mv |
Bioresources. Raleigh: North Carolina State Univ Dept Wood &paper Sci, v. 10, n. 2, p. 3197-3209, 2015. 1930-2126 WOS:000354642000098 WOS000354642000098.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioresources 1.202 0,405 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3197-3209 application/pdf |
dc.publisher.none.fl_str_mv |
North Carolina State Univ Dept Wood &paper Sci |
publisher.none.fl_str_mv |
North Carolina State Univ Dept Wood &paper Sci |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965478598213632 |