The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection

Detalhes bibliográficos
Autor(a) principal: Ayusso, Gabriela Miranda [UNESP]
Data de Publicação: 2023
Outros Autores: Lima, Maria Letícia Duarte [UNESP], da Silva Sanches, Paulo Ricardo [UNESP], Santos, Igor Andrade, Martins, Daniel Oliveira Silva [UNESP], da Conceição, Pâmela Jóyce Previdelli [UNESP], Carvalho, Tamara [UNESP], da Costa, Vivaldo Gomes [UNESP], Bittar, Cíntia [UNESP], Merits, Andres, Santos-Filho, Norival Alves [UNESP], Cilli, Eduardo Maffud [UNESP], Jardim, Ana Carolina Gomes [UNESP], de Freitas Calmon, Marilia [UNESP], Rahal, Paula [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/v15051168
http://hdl.handle.net/11449/250014
Resumo: Chikungunya virus (CHIKV) and Zika virus (ZIKV) are important disease-causing agents worldwide. Currently, there are no antiviral drugs or vaccines approved to treat these viruses. However, peptides have shown great potential for new drug development. A recent study described (p-BthTX-I)2K [(KKYRYHLKPF)2K], a peptide derived from the Bothropstoxin-I toxin in the venom of the Bothrops jararacussu snake, showed antiviral activity against SARS-CoV-2. In this study, we assessed the activity of this peptide against CHIKV and ZIKV and its antiviral action in the different stages of the viral replication cycle in vitro. We observed that (p-BthTX-I)2K impaired CHIKV infection by interfering with the early steps of the viral replication cycle, reducing CHIKV entry into BHK-21 cells specifically by reducing both the attachment and internalization steps. (p-BthTX-I)2K also inhibited the ZIKV replicative cycle in Vero cells. The peptide protected the cells against ZIKV infection and decreased the levels of the viral RNA and the NS3 protein of this virus at viral post-entry steps. In conclusion, this study highlights the potential of the (p-BthTX-I)2K peptide to be a novel broad-spectrum antiviral candidate that targets different steps of the replication cycle of both CHIKV and ZIKV.
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spelling The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus InfectionantiviralCHIKVpeptideZIKVChikungunya virus (CHIKV) and Zika virus (ZIKV) are important disease-causing agents worldwide. Currently, there are no antiviral drugs or vaccines approved to treat these viruses. However, peptides have shown great potential for new drug development. A recent study described (p-BthTX-I)2K [(KKYRYHLKPF)2K], a peptide derived from the Bothropstoxin-I toxin in the venom of the Bothrops jararacussu snake, showed antiviral activity against SARS-CoV-2. In this study, we assessed the activity of this peptide against CHIKV and ZIKV and its antiviral action in the different stages of the viral replication cycle in vitro. We observed that (p-BthTX-I)2K impaired CHIKV infection by interfering with the early steps of the viral replication cycle, reducing CHIKV entry into BHK-21 cells specifically by reducing both the attachment and internalization steps. (p-BthTX-I)2K also inhibited the ZIKV replicative cycle in Vero cells. The peptide protected the cells against ZIKV infection and decreased the levels of the viral RNA and the NS3 protein of this virus at viral post-entry steps. In conclusion, this study highlights the potential of the (p-BthTX-I)2K peptide to be a novel broad-spectrum antiviral candidate that targets different steps of the replication cycle of both CHIKV and ZIKV.Institute of Biosciences Letters and Exact Sciences São Paulo State University, SPSchool of Pharmaceutical Sciences São Paulo State University, SPInstitute of Biomedical Sciences Federal University of Uberlândia, MGLaboratory of Molecular Immunology The Rockefeller UniversityInstitute of Technology University of TartuInstitute of Chemistry São Paulo State University, SPInstitute of Biosciences Letters and Exact Sciences São Paulo State University, SPSchool of Pharmaceutical Sciences São Paulo State University, SPInstitute of Chemistry São Paulo State University, SPUniversidade Estadual Paulista (UNESP)Universidade Federal de Uberlândia (UFU)The Rockefeller UniversityUniversity of TartuAyusso, Gabriela Miranda [UNESP]Lima, Maria Letícia Duarte [UNESP]da Silva Sanches, Paulo Ricardo [UNESP]Santos, Igor AndradeMartins, Daniel Oliveira Silva [UNESP]da Conceição, Pâmela Jóyce Previdelli [UNESP]Carvalho, Tamara [UNESP]da Costa, Vivaldo Gomes [UNESP]Bittar, Cíntia [UNESP]Merits, AndresSantos-Filho, Norival Alves [UNESP]Cilli, Eduardo Maffud [UNESP]Jardim, Ana Carolina Gomes [UNESP]de Freitas Calmon, Marilia [UNESP]Rahal, Paula [UNESP]2023-07-29T16:15:28Z2023-07-29T16:15:28Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/v15051168Viruses, v. 15, n. 5, 2023.1999-4915http://hdl.handle.net/11449/25001410.3390/v150511682-s2.0-85160603859Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirusesinfo:eu-repo/semantics/openAccess2023-07-29T16:15:28Zoai:repositorio.unesp.br:11449/250014Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:05.768982Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
title The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
spellingShingle The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
Ayusso, Gabriela Miranda [UNESP]
antiviral
CHIKV
peptide
ZIKV
title_short The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
title_full The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
title_fullStr The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
title_full_unstemmed The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
title_sort The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection
author Ayusso, Gabriela Miranda [UNESP]
author_facet Ayusso, Gabriela Miranda [UNESP]
Lima, Maria Letícia Duarte [UNESP]
da Silva Sanches, Paulo Ricardo [UNESP]
Santos, Igor Andrade
Martins, Daniel Oliveira Silva [UNESP]
da Conceição, Pâmela Jóyce Previdelli [UNESP]
Carvalho, Tamara [UNESP]
da Costa, Vivaldo Gomes [UNESP]
Bittar, Cíntia [UNESP]
Merits, Andres
Santos-Filho, Norival Alves [UNESP]
Cilli, Eduardo Maffud [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
de Freitas Calmon, Marilia [UNESP]
Rahal, Paula [UNESP]
author_role author
author2 Lima, Maria Letícia Duarte [UNESP]
da Silva Sanches, Paulo Ricardo [UNESP]
Santos, Igor Andrade
Martins, Daniel Oliveira Silva [UNESP]
da Conceição, Pâmela Jóyce Previdelli [UNESP]
Carvalho, Tamara [UNESP]
da Costa, Vivaldo Gomes [UNESP]
Bittar, Cíntia [UNESP]
Merits, Andres
Santos-Filho, Norival Alves [UNESP]
Cilli, Eduardo Maffud [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
de Freitas Calmon, Marilia [UNESP]
Rahal, Paula [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Uberlândia (UFU)
The Rockefeller University
University of Tartu
dc.contributor.author.fl_str_mv Ayusso, Gabriela Miranda [UNESP]
Lima, Maria Letícia Duarte [UNESP]
da Silva Sanches, Paulo Ricardo [UNESP]
Santos, Igor Andrade
Martins, Daniel Oliveira Silva [UNESP]
da Conceição, Pâmela Jóyce Previdelli [UNESP]
Carvalho, Tamara [UNESP]
da Costa, Vivaldo Gomes [UNESP]
Bittar, Cíntia [UNESP]
Merits, Andres
Santos-Filho, Norival Alves [UNESP]
Cilli, Eduardo Maffud [UNESP]
Jardim, Ana Carolina Gomes [UNESP]
de Freitas Calmon, Marilia [UNESP]
Rahal, Paula [UNESP]
dc.subject.por.fl_str_mv antiviral
CHIKV
peptide
ZIKV
topic antiviral
CHIKV
peptide
ZIKV
description Chikungunya virus (CHIKV) and Zika virus (ZIKV) are important disease-causing agents worldwide. Currently, there are no antiviral drugs or vaccines approved to treat these viruses. However, peptides have shown great potential for new drug development. A recent study described (p-BthTX-I)2K [(KKYRYHLKPF)2K], a peptide derived from the Bothropstoxin-I toxin in the venom of the Bothrops jararacussu snake, showed antiviral activity against SARS-CoV-2. In this study, we assessed the activity of this peptide against CHIKV and ZIKV and its antiviral action in the different stages of the viral replication cycle in vitro. We observed that (p-BthTX-I)2K impaired CHIKV infection by interfering with the early steps of the viral replication cycle, reducing CHIKV entry into BHK-21 cells specifically by reducing both the attachment and internalization steps. (p-BthTX-I)2K also inhibited the ZIKV replicative cycle in Vero cells. The peptide protected the cells against ZIKV infection and decreased the levels of the viral RNA and the NS3 protein of this virus at viral post-entry steps. In conclusion, this study highlights the potential of the (p-BthTX-I)2K peptide to be a novel broad-spectrum antiviral candidate that targets different steps of the replication cycle of both CHIKV and ZIKV.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:15:28Z
2023-07-29T16:15:28Z
2023-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/v15051168
Viruses, v. 15, n. 5, 2023.
1999-4915
http://hdl.handle.net/11449/250014
10.3390/v15051168
2-s2.0-85160603859
url http://dx.doi.org/10.3390/v15051168
http://hdl.handle.net/11449/250014
identifier_str_mv Viruses, v. 15, n. 5, 2023.
1999-4915
10.3390/v15051168
2-s2.0-85160603859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viruses
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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