Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12906-020-03190-1 http://hdl.handle.net/11449/209948 |
Resumo: | Background Cancer is a multifactorial disease caused by uncontrolled proliferation of cells. About 50-80% of cancer patients develop cachexia, a complex metabolic syndrome associated with an increase of mortality and morbidity. However, there are no effective therapies in medical clinic for cancer cachexia. Vochysia tucanorum Mart. is a common three of the Brazilian Cerrado. The butanolic fraction of V. tucanorum (Fr-BuVt), very rich in triterpenes with various biological activities, might be interesting in being tested in cancer cachexia syndrome. Hence, the present study was undertaken to investigate the antitumoral activity of Fr-BuVt and its potential against cachexia development. Methods Ehrlich tumor was used as model of cancer cachexia. Ascitic Ehrlich tumor cells were collected, processed and inoculated subcutaneously in saline solution (1 x 10(7)/100 mu l; >= 95% viability) for the obtention of solid Ehrlich carcinoma. After inoculation, solid Ehrlich carcinoma-bearing mice were treated by 14 consecutive days by gavage with Fr-BuVt (200 mg/kg). Body weight and tumor volume were measure during the treatment period. Tumors were removed, weighed and properly processed to measure the content and phosphorylation levels of key-proteins involved to apoptotic and proliferation process by Western Blot. Muscles and adipose tissues were removed for weighed. Serum was collected to cytokines levels and energetic blood markers measurements. Results The treatment with the Fr-BuVt (200 mg/kg, 14 days) decreased the solid Ehrlich tumor volume and weight besides increased the expression of the pro-apoptotic proteins caspase-3 and BAX, but also decreased the expression of the proteins involved in proliferation NF kappa B, mTOR and ERK. In addition, our data shows that the administration of Fr-BuVt was able to prevent the installation of cancer cachexia in Ehrlich carcinoma-bearing mice, since prevented the loss of body weight, as well as the loss of muscle and adipose tissue. Moreover, an improvement in some blood parameters such as decrease in cytokines TNF-alpha and IL-6 levels is observed. Conclusions The study revealed that Fr-BuVt has antitumoral activity and prevent installation of cancer cachexia in Ehrlich model. Therefore, Fr-BuVt may represent an alternative treatment for cancer cachexia. |
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Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor modelAnti-apoptoticAnti-proliferationCancer cachexiaMetabolic syndromeTriterpenesBackground Cancer is a multifactorial disease caused by uncontrolled proliferation of cells. About 50-80% of cancer patients develop cachexia, a complex metabolic syndrome associated with an increase of mortality and morbidity. However, there are no effective therapies in medical clinic for cancer cachexia. Vochysia tucanorum Mart. is a common three of the Brazilian Cerrado. The butanolic fraction of V. tucanorum (Fr-BuVt), very rich in triterpenes with various biological activities, might be interesting in being tested in cancer cachexia syndrome. Hence, the present study was undertaken to investigate the antitumoral activity of Fr-BuVt and its potential against cachexia development. Methods Ehrlich tumor was used as model of cancer cachexia. Ascitic Ehrlich tumor cells were collected, processed and inoculated subcutaneously in saline solution (1 x 10(7)/100 mu l; >= 95% viability) for the obtention of solid Ehrlich carcinoma. After inoculation, solid Ehrlich carcinoma-bearing mice were treated by 14 consecutive days by gavage with Fr-BuVt (200 mg/kg). Body weight and tumor volume were measure during the treatment period. Tumors were removed, weighed and properly processed to measure the content and phosphorylation levels of key-proteins involved to apoptotic and proliferation process by Western Blot. Muscles and adipose tissues were removed for weighed. Serum was collected to cytokines levels and energetic blood markers measurements. Results The treatment with the Fr-BuVt (200 mg/kg, 14 days) decreased the solid Ehrlich tumor volume and weight besides increased the expression of the pro-apoptotic proteins caspase-3 and BAX, but also decreased the expression of the proteins involved in proliferation NF kappa B, mTOR and ERK. In addition, our data shows that the administration of Fr-BuVt was able to prevent the installation of cancer cachexia in Ehrlich carcinoma-bearing mice, since prevented the loss of body weight, as well as the loss of muscle and adipose tissue. Moreover, an improvement in some blood parameters such as decrease in cytokines TNF-alpha and IL-6 levels is observed. Conclusions The study revealed that Fr-BuVt has antitumoral activity and prevent installation of cancer cachexia in Ehrlich model. Therefore, Fr-BuVt may represent an alternative treatment for cancer cachexia.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Lab Metab Control, Ribeirao Preto, SP, BrazilSao Paulo State Univ, Dept Phys Educ, Lab Endocrine Pancreas Physiol, Fac Sci, Av Eng Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP, BrazilSao Paulo State Univ, Dept Biol Sci, Lab Nat Prod Chem, Fac Sci, Bauru, SP, BrazilSao Paulo State Univ, Dept Phys Educ, Lab Endocrine Pancreas Physiol, Fac Sci, Av Eng Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP, BrazilSao Paulo State Univ, Dept Biol Sci, Lab Nat Prod Chem, Fac Sci, Bauru, SP, BrazilFAPESP: FAPESP -2016/07866-1BmcUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Morgan, Henrique Jorge NovaesDelgado, Aislan Quintiliano [UNESP]Saldanha, Luiz Leonardo [UNESP]Camaforte, Nathalia Aparecida De Paula [UNESP]Dokkedal, Anne Ligia [UNESP]Bosqueiro, Jose Roberto [UNESP]2021-06-25T12:34:34Z2021-06-25T12:34:34Z2021-01-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.1186/s12906-020-03190-1Bmc Complementary Medicine And Therapies. London: Bmc, v. 21, n. 1, 13 p., 2021.http://hdl.handle.net/11449/20994810.1186/s12906-020-03190-1WOS:000610409300004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Complementary Medicine And Therapiesinfo:eu-repo/semantics/openAccess2024-04-24T18:53:11Zoai:repositorio.unesp.br:11449/209948Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-24T18:53:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
title |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
spellingShingle |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model Morgan, Henrique Jorge Novaes Anti-apoptotic Anti-proliferation Cancer cachexia Metabolic syndrome Triterpenes |
title_short |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
title_full |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
title_fullStr |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
title_full_unstemmed |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
title_sort |
Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model |
author |
Morgan, Henrique Jorge Novaes |
author_facet |
Morgan, Henrique Jorge Novaes Delgado, Aislan Quintiliano [UNESP] Saldanha, Luiz Leonardo [UNESP] Camaforte, Nathalia Aparecida De Paula [UNESP] Dokkedal, Anne Ligia [UNESP] Bosqueiro, Jose Roberto [UNESP] |
author_role |
author |
author2 |
Delgado, Aislan Quintiliano [UNESP] Saldanha, Luiz Leonardo [UNESP] Camaforte, Nathalia Aparecida De Paula [UNESP] Dokkedal, Anne Ligia [UNESP] Bosqueiro, Jose Roberto [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Morgan, Henrique Jorge Novaes Delgado, Aislan Quintiliano [UNESP] Saldanha, Luiz Leonardo [UNESP] Camaforte, Nathalia Aparecida De Paula [UNESP] Dokkedal, Anne Ligia [UNESP] Bosqueiro, Jose Roberto [UNESP] |
dc.subject.por.fl_str_mv |
Anti-apoptotic Anti-proliferation Cancer cachexia Metabolic syndrome Triterpenes |
topic |
Anti-apoptotic Anti-proliferation Cancer cachexia Metabolic syndrome Triterpenes |
description |
Background Cancer is a multifactorial disease caused by uncontrolled proliferation of cells. About 50-80% of cancer patients develop cachexia, a complex metabolic syndrome associated with an increase of mortality and morbidity. However, there are no effective therapies in medical clinic for cancer cachexia. Vochysia tucanorum Mart. is a common three of the Brazilian Cerrado. The butanolic fraction of V. tucanorum (Fr-BuVt), very rich in triterpenes with various biological activities, might be interesting in being tested in cancer cachexia syndrome. Hence, the present study was undertaken to investigate the antitumoral activity of Fr-BuVt and its potential against cachexia development. Methods Ehrlich tumor was used as model of cancer cachexia. Ascitic Ehrlich tumor cells were collected, processed and inoculated subcutaneously in saline solution (1 x 10(7)/100 mu l; >= 95% viability) for the obtention of solid Ehrlich carcinoma. After inoculation, solid Ehrlich carcinoma-bearing mice were treated by 14 consecutive days by gavage with Fr-BuVt (200 mg/kg). Body weight and tumor volume were measure during the treatment period. Tumors were removed, weighed and properly processed to measure the content and phosphorylation levels of key-proteins involved to apoptotic and proliferation process by Western Blot. Muscles and adipose tissues were removed for weighed. Serum was collected to cytokines levels and energetic blood markers measurements. Results The treatment with the Fr-BuVt (200 mg/kg, 14 days) decreased the solid Ehrlich tumor volume and weight besides increased the expression of the pro-apoptotic proteins caspase-3 and BAX, but also decreased the expression of the proteins involved in proliferation NF kappa B, mTOR and ERK. In addition, our data shows that the administration of Fr-BuVt was able to prevent the installation of cancer cachexia in Ehrlich carcinoma-bearing mice, since prevented the loss of body weight, as well as the loss of muscle and adipose tissue. Moreover, an improvement in some blood parameters such as decrease in cytokines TNF-alpha and IL-6 levels is observed. Conclusions The study revealed that Fr-BuVt has antitumoral activity and prevent installation of cancer cachexia in Ehrlich model. Therefore, Fr-BuVt may represent an alternative treatment for cancer cachexia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:34:34Z 2021-06-25T12:34:34Z 2021-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12906-020-03190-1 Bmc Complementary Medicine And Therapies. London: Bmc, v. 21, n. 1, 13 p., 2021. http://hdl.handle.net/11449/209948 10.1186/s12906-020-03190-1 WOS:000610409300004 |
url |
http://dx.doi.org/10.1186/s12906-020-03190-1 http://hdl.handle.net/11449/209948 |
identifier_str_mv |
Bmc Complementary Medicine And Therapies. London: Bmc, v. 21, n. 1, 13 p., 2021. 10.1186/s12906-020-03190-1 WOS:000610409300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Complementary Medicine And Therapies |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Bmc |
publisher.none.fl_str_mv |
Bmc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046549639397376 |