Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0037694 http://hdl.handle.net/11449/18741 |
Resumo: | Background: Paracoccidioidomycosis is a systemic mycosis caused by Paracoccidioides brasiliensis (species S1, PS2, PS3), and Paracoccidioides lutzii. This work aimed to differentiate species within the genus Paracoccidioides, without applying multilocus sequencing, as well as to obtain knowledge of the possible speciation processes.Methodology/Principal Findings: Single nucleotide polymorphism analysis on GP43, ARF and PRP8 intein genes successfully distinguished isolates into four different species. Morphological evaluation indicated that elongated conidia were observed exclusively in P. lutzii isolates, while all other species (S1, PS2 and PS3) were indistinguishable. To evaluate the biogeographic events that led to the current geographic distribution of Paracoccidioides species and their sister species, Nested Clade and Likelihood Analysis of Geographic Range Evolution (LAGRANGE) analyses were applied. The radiation of Paracoccidioides started in northwest South America, around 11-32 million years ago, as calculated on the basis of ARF substitution rate, in the BEAST program. Vicariance was responsible for the divergence among S1, PS2 and P. lutzii and a recent dispersal generated the PS3 species, restricted to Colombia. Taking into account the ancestral areas revealed by the LAGRANGE analysis and the major geographic distribution of L. loboi in the Amazon basin, a region strongly affected by the Andes uplift and marine incursions in the Cenozoic era, we also speculate about the effect of these geological events on the vicariance between Paracoccidioides and L. loboi.Conclusions/Significance: The use of at least 3 SNPs, but not morphological criteria, as markers allows us to distinguish among the four cryptic species of the genus Paracoccidioides. The work also presents a biogeographic study speculating on how these species might have diverged in South America, thus contributing to elucidating evolutionary aspects of the genus Paracoccidioides. |
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Genus Paracoccidioides: Species Recognition and Biogeographic AspectsBackground: Paracoccidioidomycosis is a systemic mycosis caused by Paracoccidioides brasiliensis (species S1, PS2, PS3), and Paracoccidioides lutzii. This work aimed to differentiate species within the genus Paracoccidioides, without applying multilocus sequencing, as well as to obtain knowledge of the possible speciation processes.Methodology/Principal Findings: Single nucleotide polymorphism analysis on GP43, ARF and PRP8 intein genes successfully distinguished isolates into four different species. Morphological evaluation indicated that elongated conidia were observed exclusively in P. lutzii isolates, while all other species (S1, PS2 and PS3) were indistinguishable. To evaluate the biogeographic events that led to the current geographic distribution of Paracoccidioides species and their sister species, Nested Clade and Likelihood Analysis of Geographic Range Evolution (LAGRANGE) analyses were applied. The radiation of Paracoccidioides started in northwest South America, around 11-32 million years ago, as calculated on the basis of ARF substitution rate, in the BEAST program. Vicariance was responsible for the divergence among S1, PS2 and P. lutzii and a recent dispersal generated the PS3 species, restricted to Colombia. Taking into account the ancestral areas revealed by the LAGRANGE analysis and the major geographic distribution of L. loboi in the Amazon basin, a region strongly affected by the Andes uplift and marine incursions in the Cenozoic era, we also speculate about the effect of these geological events on the vicariance between Paracoccidioides and L. loboi.Conclusions/Significance: The use of at least 3 SNPs, but not morphological criteria, as markers allows us to distinguish among the four cryptic species of the genus Paracoccidioides. The work also presents a biogeographic study speculating on how these species might have diverged in South America, thus contributing to elucidating evolutionary aspects of the genus Paracoccidioides.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, São Paulo, BrazilUniv Brasilia UNB, Brasilia, DF, BrazilCtr Microbiol & Cell Biol, Venezuelan Inst Sci Res, Caracas, VenezuelaUniv Estadual Paulista, São Paulo, BrazilFAPESP: 07/01306-5FAPESP: 10/08839-1FAPESP: 10/08829-6Public Library ScienceUniversidade Estadual Paulista (Unesp)Universidade de Brasília (UnB)Ctr Microbiol & Cell BiolTheodoro, Raquel Cordeiro [UNESP]Teixeira, Marcus de MeloSoares Felipe, Maria Suelidos Santos Paduan, Karina [UNESP]Ribolla, Paulo Martins [UNESP]San-Blas, GiocondaBagagli, Eduardo [UNESP]2014-05-20T13:52:28Z2014-05-20T13:52:28Z2012-05-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://dx.doi.org/10.1371/journal.pone.0037694Plos One. San Francisco: Public Library Science, v. 7, n. 5, p. 15, 2012.1932-6203http://hdl.handle.net/11449/1874110.1371/journal.pone.0037694WOS:000305353400041WOS000305353400041.pdf332032757042953935771497484568800000-0001-8735-60900000-0002-8003-4109Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-11-02T06:07:13Zoai:repositorio.unesp.br:11449/18741Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:42:14.187306Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
title |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
spellingShingle |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects Theodoro, Raquel Cordeiro [UNESP] |
title_short |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
title_full |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
title_fullStr |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
title_full_unstemmed |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
title_sort |
Genus Paracoccidioides: Species Recognition and Biogeographic Aspects |
author |
Theodoro, Raquel Cordeiro [UNESP] |
author_facet |
Theodoro, Raquel Cordeiro [UNESP] Teixeira, Marcus de Melo Soares Felipe, Maria Sueli dos Santos Paduan, Karina [UNESP] Ribolla, Paulo Martins [UNESP] San-Blas, Gioconda Bagagli, Eduardo [UNESP] |
author_role |
author |
author2 |
Teixeira, Marcus de Melo Soares Felipe, Maria Sueli dos Santos Paduan, Karina [UNESP] Ribolla, Paulo Martins [UNESP] San-Blas, Gioconda Bagagli, Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Brasília (UnB) Ctr Microbiol & Cell Biol |
dc.contributor.author.fl_str_mv |
Theodoro, Raquel Cordeiro [UNESP] Teixeira, Marcus de Melo Soares Felipe, Maria Sueli dos Santos Paduan, Karina [UNESP] Ribolla, Paulo Martins [UNESP] San-Blas, Gioconda Bagagli, Eduardo [UNESP] |
description |
Background: Paracoccidioidomycosis is a systemic mycosis caused by Paracoccidioides brasiliensis (species S1, PS2, PS3), and Paracoccidioides lutzii. This work aimed to differentiate species within the genus Paracoccidioides, without applying multilocus sequencing, as well as to obtain knowledge of the possible speciation processes.Methodology/Principal Findings: Single nucleotide polymorphism analysis on GP43, ARF and PRP8 intein genes successfully distinguished isolates into four different species. Morphological evaluation indicated that elongated conidia were observed exclusively in P. lutzii isolates, while all other species (S1, PS2 and PS3) were indistinguishable. To evaluate the biogeographic events that led to the current geographic distribution of Paracoccidioides species and their sister species, Nested Clade and Likelihood Analysis of Geographic Range Evolution (LAGRANGE) analyses were applied. The radiation of Paracoccidioides started in northwest South America, around 11-32 million years ago, as calculated on the basis of ARF substitution rate, in the BEAST program. Vicariance was responsible for the divergence among S1, PS2 and P. lutzii and a recent dispersal generated the PS3 species, restricted to Colombia. Taking into account the ancestral areas revealed by the LAGRANGE analysis and the major geographic distribution of L. loboi in the Amazon basin, a region strongly affected by the Andes uplift and marine incursions in the Cenozoic era, we also speculate about the effect of these geological events on the vicariance between Paracoccidioides and L. loboi.Conclusions/Significance: The use of at least 3 SNPs, but not morphological criteria, as markers allows us to distinguish among the four cryptic species of the genus Paracoccidioides. The work also presents a biogeographic study speculating on how these species might have diverged in South America, thus contributing to elucidating evolutionary aspects of the genus Paracoccidioides. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-30 2014-05-20T13:52:28Z 2014-05-20T13:52:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0037694 Plos One. San Francisco: Public Library Science, v. 7, n. 5, p. 15, 2012. 1932-6203 http://hdl.handle.net/11449/18741 10.1371/journal.pone.0037694 WOS:000305353400041 WOS000305353400041.pdf 3320327570429539 3577149748456880 0000-0001-8735-6090 0000-0002-8003-4109 |
url |
http://dx.doi.org/10.1371/journal.pone.0037694 http://hdl.handle.net/11449/18741 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 7, n. 5, p. 15, 2012. 1932-6203 10.1371/journal.pone.0037694 WOS:000305353400041 WOS000305353400041.pdf 3320327570429539 3577149748456880 0000-0001-8735-6090 0000-0002-8003-4109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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15 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128688244391936 |