Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and grastic cancer

• Autor(a) principal: Duarte, Márcia Cristina [UNESP]
• Data de Publicação: 2005
• Outros Autores: Colombo, Jucimara [UNESP], Rossit, Andrea Regina Baptista, Caetano, Alaor, Borim, Aldenis Albaneze, Wornrath, Durval, Silva, Ana Elizabete [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.wjgnet.com/1007-9327/abstract_en.asp?f=6593&v=11; http://hdl.handle.net/11449/68491
• topic: Environmental exposure; Gastric cancer; Gastritis; Polymorphism; XRCC1; XRCC3; arginine; DNA; DNA binding protein; glycine; methionine; protein XRCC1; protein XRCC3; threonine; tryptophan; unclassified drug; adult; aged; allele; Brazil; chronic gastritis; cigarette smoking; codon; controlled study; data analysis; demography; disease predisposition; DNA repair; drinking behavior; environmental exposure; environmental factor; female; gender; genetic polymorphism; genetic variability; genotype; genotype environment interaction; Helicobacter infection; Helicobacter pylori; human; major clinical study; male; nonhuman; polymerase chain reaction; population research; precancer; restriction fragment length polymorphism; risk assessment; stomach cancer; Adult; Aged; Aged, 80 and over; Case-Control Studies; Chronic Disease; DNA Repair; DNA-Binding Proteins; Environmental Exposure; Female; Genetic Predisposition to Disease; Genotype; Humans; Male; Middle Aged; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Risk Factors; Stomach Neoplasms

Aim: To evaluate the association between polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population. Methods: Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis (CG) and 160 patients with gastric cancer (GC), matched to 202 (C1) and 150 (C2) controls, respectively. Results: No differences were observed among the studied groups with regard to the genotype distribution of XRCC1 codons 194 and 399 and of XRCC3 codon 241. However, the combined analyses of the three variant alleles (194Trp, 399Gln and 241Met) showed an increased risk for chronic gastritis when compared to the GC group. Moreover, an interaction between the polymorphic alleles and demographic and environmental factors was observed in the CG and GC groups. XRCC1 194Trp was associated with smoking in the CG group, while the variant alleles XRCC1 399Gln and XRCC3 241Met were related with gender, smoking, drinking and H pylori infection in the CG and GC groups. Conclusion: Our results showed no evidence of a rela-tionship between the polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk of chronic gastritis and gastric cancer in the Brazilian population, but the combined effect of these variants may interact to increase the risk for chronic gastritis, considered a premalignant lesion. Our data also indicate a gene-environment interaction in the susceptibility to chronic gastritis and gastric cancer. © 2005 The WJG Press and Elsevier Inc. All rights reserved.