Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A

• Autor(a) principal: Dellaqua, Thaisy Tino [UNESP]
• Data de Publicação: 2023
• Outros Autores: Franchi, Fernanda Fagali [UNESP], dos Santos, Priscila Helena [UNESP], Giroto, Alan Brunholi, Nunes, Sarah Gomes [UNESP], de Lima, Victor Augusto Vieira, Guilherme, Vitória Bertogna [UNESP], Fontes, Patrícia Kubo [UNESP], Sudano, Mateus José, de Souza Castilho, Anthony César
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1007/s11259-023-10072-7; http://hdl.handle.net/11449/246662
• topic: Blastocyst; Cattle; Gene expression; Lipid content; Oocyte in vitro maturation; PAPP-A

Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using in vitro-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (P > 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating PPARGC1A and AKR1B1, which are related to lipid metabolism; CASP9, a pro-apoptotic gene; and IFN-τ, a marker of embryo quality (P < 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (P < 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A’s approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts.