Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/bioengineering10010015 http://hdl.handle.net/11449/246716 |
Resumo: | The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential. |
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Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagenbiomaterialbone tissuemembraneregenerationThe membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPFAPESP: 2021/00939-1Universidade Estadual Paulista (UNESP)de Oliveira, Júlio César Silva [UNESP]Baggio, Ana Maira Pereira [UNESP]Benetti, Luan Pier [UNESP]Delamura, Izabela Fornazari [UNESP]Ramos, Edith Umasi [UNESP]Bizelli, Vinícius Ferreira [UNESP]Bassi, Ana Paula Farnezi [UNESP]2023-07-29T12:48:34Z2023-07-29T12:48:34Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/bioengineering10010015Bioengineering, v. 10, n. 1, 2023.2306-5354http://hdl.handle.net/11449/24671610.3390/bioengineering100100152-s2.0-85146782669Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioengineeringinfo:eu-repo/semantics/openAccess2023-07-29T12:48:34Zoai:repositorio.unesp.br:11449/246716Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:47:14.002886Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
spellingShingle |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen de Oliveira, Júlio César Silva [UNESP] biomaterial bone tissue membrane regeneration |
title_short |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_full |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_fullStr |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_full_unstemmed |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
title_sort |
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen |
author |
de Oliveira, Júlio César Silva [UNESP] |
author_facet |
de Oliveira, Júlio César Silva [UNESP] Baggio, Ana Maira Pereira [UNESP] Benetti, Luan Pier [UNESP] Delamura, Izabela Fornazari [UNESP] Ramos, Edith Umasi [UNESP] Bizelli, Vinícius Ferreira [UNESP] Bassi, Ana Paula Farnezi [UNESP] |
author_role |
author |
author2 |
Baggio, Ana Maira Pereira [UNESP] Benetti, Luan Pier [UNESP] Delamura, Izabela Fornazari [UNESP] Ramos, Edith Umasi [UNESP] Bizelli, Vinícius Ferreira [UNESP] Bassi, Ana Paula Farnezi [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Oliveira, Júlio César Silva [UNESP] Baggio, Ana Maira Pereira [UNESP] Benetti, Luan Pier [UNESP] Delamura, Izabela Fornazari [UNESP] Ramos, Edith Umasi [UNESP] Bizelli, Vinícius Ferreira [UNESP] Bassi, Ana Paula Farnezi [UNESP] |
dc.subject.por.fl_str_mv |
biomaterial bone tissue membrane regeneration |
topic |
biomaterial bone tissue membrane regeneration |
description |
The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:48:34Z 2023-07-29T12:48:34Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/bioengineering10010015 Bioengineering, v. 10, n. 1, 2023. 2306-5354 http://hdl.handle.net/11449/246716 10.3390/bioengineering10010015 2-s2.0-85146782669 |
url |
http://dx.doi.org/10.3390/bioengineering10010015 http://hdl.handle.net/11449/246716 |
identifier_str_mv |
Bioengineering, v. 10, n. 1, 2023. 2306-5354 10.3390/bioengineering10010015 2-s2.0-85146782669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioengineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128858199687168 |