Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Júlio César Silva [UNESP]
Data de Publicação: 2023
Outros Autores: Baggio, Ana Maira Pereira [UNESP], Benetti, Luan Pier [UNESP], Delamura, Izabela Fornazari [UNESP], Ramos, Edith Umasi [UNESP], Bizelli, Vinícius Ferreira [UNESP], Bassi, Ana Paula Farnezi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/bioengineering10010015
http://hdl.handle.net/11449/246716
Resumo: The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.
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spelling Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagenbiomaterialbone tissuemembraneregenerationThe membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, SPFAPESP: 2021/00939-1Universidade Estadual Paulista (UNESP)de Oliveira, Júlio César Silva [UNESP]Baggio, Ana Maira Pereira [UNESP]Benetti, Luan Pier [UNESP]Delamura, Izabela Fornazari [UNESP]Ramos, Edith Umasi [UNESP]Bizelli, Vinícius Ferreira [UNESP]Bassi, Ana Paula Farnezi [UNESP]2023-07-29T12:48:34Z2023-07-29T12:48:34Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/bioengineering10010015Bioengineering, v. 10, n. 1, 2023.2306-5354http://hdl.handle.net/11449/24671610.3390/bioengineering100100152-s2.0-85146782669Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioengineeringinfo:eu-repo/semantics/openAccess2023-07-29T12:48:34Zoai:repositorio.unesp.br:11449/246716Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:47:14.002886Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
title Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
spellingShingle Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
de Oliveira, Júlio César Silva [UNESP]
biomaterial
bone tissue
membrane
regeneration
title_short Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
title_full Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
title_fullStr Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
title_full_unstemmed Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
title_sort Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
author de Oliveira, Júlio César Silva [UNESP]
author_facet de Oliveira, Júlio César Silva [UNESP]
Baggio, Ana Maira Pereira [UNESP]
Benetti, Luan Pier [UNESP]
Delamura, Izabela Fornazari [UNESP]
Ramos, Edith Umasi [UNESP]
Bizelli, Vinícius Ferreira [UNESP]
Bassi, Ana Paula Farnezi [UNESP]
author_role author
author2 Baggio, Ana Maira Pereira [UNESP]
Benetti, Luan Pier [UNESP]
Delamura, Izabela Fornazari [UNESP]
Ramos, Edith Umasi [UNESP]
Bizelli, Vinícius Ferreira [UNESP]
Bassi, Ana Paula Farnezi [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv de Oliveira, Júlio César Silva [UNESP]
Baggio, Ana Maira Pereira [UNESP]
Benetti, Luan Pier [UNESP]
Delamura, Izabela Fornazari [UNESP]
Ramos, Edith Umasi [UNESP]
Bizelli, Vinícius Ferreira [UNESP]
Bassi, Ana Paula Farnezi [UNESP]
dc.subject.por.fl_str_mv biomaterial
bone tissue
membrane
regeneration
topic biomaterial
bone tissue
membrane
regeneration
description The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:48:34Z
2023-07-29T12:48:34Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/bioengineering10010015
Bioengineering, v. 10, n. 1, 2023.
2306-5354
http://hdl.handle.net/11449/246716
10.3390/bioengineering10010015
2-s2.0-85146782669
url http://dx.doi.org/10.3390/bioengineering10010015
http://hdl.handle.net/11449/246716
identifier_str_mv Bioengineering, v. 10, n. 1, 2023.
2306-5354
10.3390/bioengineering10010015
2-s2.0-85146782669
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioengineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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