Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains

Detalhes bibliográficos
Autor(a) principal: Fortaleza, Carlos Magno Castelo Branco [UNESP]
Data de Publicação: 2011
Outros Autores: Bacchi, C. E. [UNESP], Oliveira, Deilson Elgui de [UNESP], Ramos, M. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992011000200008
http://hdl.handle.net/11449/11800
Resumo: Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for nosocomial strains varied from 0.14 to 1.00 (Dice); 0.08 to 1.00 (Jaccard) and 0.58 to 1.00 (simple matching). Forty patterns were identified. In most units, several clones coexisted. However, there was evidence of clonal dissemination in the high risk nursery, neurology and two surgical units. Each and every community-acquired strain produced a unique distinct pattern. Results suggest that cross transmission of P. aeruginosa was an uncommon event in our hospital. This points out to a minor role for barrier methods in the control of P. aeruginosa spread.
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spelling Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strainsPseudomonas aeruginosastrain typingERIC-PCRnosocomial infectionshealth care infectionsPseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for nosocomial strains varied from 0.14 to 1.00 (Dice); 0.08 to 1.00 (Jaccard) and 0.58 to 1.00 (simple matching). Forty patterns were identified. In most units, several clones coexisted. However, there was evidence of clonal dissemination in the high risk nursery, neurology and two surgical units. Each and every community-acquired strain produced a unique distinct pattern. Results suggest that cross transmission of P. aeruginosa was an uncommon event in our hospital. This points out to a minor role for barrier methods in the control of P. aeruginosa spread.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, São Paulo State Univ, Botucatu Med Sch, Dept Trop Dis & Imaging Diag, BR-18618970 Botucatu, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Campinas, State Univ Campinas, Sch Med Sci, Campinas, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Botucatu Med Sch, Dept Trop Dis & Imaging Diag, BR-18618970 Botucatu, SP, BrazilUNESP Univ Estadual Paulista, São Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP, BrazilUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Fortaleza, Carlos Magno Castelo Branco [UNESP]Bacchi, C. E. [UNESP]Oliveira, Deilson Elgui de [UNESP]Ramos, M. C.2014-05-20T13:34:26Z2014-05-20T13:34:26Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article176-183application/pdfhttp://dx.doi.org/10.1590/S1678-91992011000200008Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 2, p. 176-183, 2011.1678-9199http://hdl.handle.net/11449/11800S1678-91992011000200008WOS:000290866500008S1678-91992011000200008-en.pdf5240998569868081Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2024-09-03T13:14:32Zoai:repositorio.unesp.br:11449/11800Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
title Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
spellingShingle Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
Fortaleza, Carlos Magno Castelo Branco [UNESP]
Pseudomonas aeruginosa
strain typing
ERIC-PCR
nosocomial infections
health care infections
title_short Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
title_full Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
title_fullStr Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
title_full_unstemmed Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
title_sort Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
author Fortaleza, Carlos Magno Castelo Branco [UNESP]
author_facet Fortaleza, Carlos Magno Castelo Branco [UNESP]
Bacchi, C. E. [UNESP]
Oliveira, Deilson Elgui de [UNESP]
Ramos, M. C.
author_role author
author2 Bacchi, C. E. [UNESP]
Oliveira, Deilson Elgui de [UNESP]
Ramos, M. C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Fortaleza, Carlos Magno Castelo Branco [UNESP]
Bacchi, C. E. [UNESP]
Oliveira, Deilson Elgui de [UNESP]
Ramos, M. C.
dc.subject.por.fl_str_mv Pseudomonas aeruginosa
strain typing
ERIC-PCR
nosocomial infections
health care infections
topic Pseudomonas aeruginosa
strain typing
ERIC-PCR
nosocomial infections
health care infections
description Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for nosocomial strains varied from 0.14 to 1.00 (Dice); 0.08 to 1.00 (Jaccard) and 0.58 to 1.00 (simple matching). Forty patterns were identified. In most units, several clones coexisted. However, there was evidence of clonal dissemination in the high risk nursery, neurology and two surgical units. Each and every community-acquired strain produced a unique distinct pattern. Results suggest that cross transmission of P. aeruginosa was an uncommon event in our hospital. This points out to a minor role for barrier methods in the control of P. aeruginosa spread.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2014-05-20T13:34:26Z
2014-05-20T13:34:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992011000200008
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 2, p. 176-183, 2011.
1678-9199
http://hdl.handle.net/11449/11800
S1678-91992011000200008
WOS:000290866500008
S1678-91992011000200008-en.pdf
5240998569868081
url http://dx.doi.org/10.1590/S1678-91992011000200008
http://hdl.handle.net/11449/11800
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 2, p. 176-183, 2011.
1678-9199
S1678-91992011000200008
WOS:000290866500008
S1678-91992011000200008-en.pdf
5240998569868081
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
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0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 176-183
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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