Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Andrea F. [UNESP]
Data de Publicação: 2018
Outros Autores: Polegato, Bertha F. [UNESP], Fernandes, Ana Angélica [UNESP], Ishikawa, Larissa Lumi [UNESP], Okoshi, Katashi [UNESP], Bazan, Silméia G. Z. [UNESP], Minicucci, Marcos F. [UNESP], Azevedo, Paula S. [UNESP], Ikoma, Maura R., Penitenti, Marcimara, Chiuso-Minicucci, Fernanda [UNESP], R Paiva, Sergio A. [UNESP], Zornoff, Leonardo A. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000494011
http://hdl.handle.net/11449/188165
Resumo: Background/Aims: The objective of our study was to evaluate the effects of zinc supplementation on cardiac remodeling following acute myocardial infarction in rats. Methods: Animals were subdivided into 4 groups and observed for 3 months: 1) Sham Control; 2) Sham Zinc: Sham animals receiving zinc supplementation; 3) Infarction Control; 4) Infarction Zinc. After the followup period, we studied hypertrophy and ventricular geometry, functional alterations in vivo and in vitro, changes related to collagen, oxidative stress, and inflammation, assessed by echocardiogram, isolated heart study, western blot, flow cytometer, morphometry, and spectrophotometry. Results: Infarction induced a significant worsening of the functional variables. On the other hand, zinc attenuated both systolic and diastolic cardiac dysfunction induced by infarction. Considering the infarct size, there was no difference between the groups. Catalase and superoxide dismutase decreased in infarcted animals, and zinc increased its activity. We found higher expression of collagens I and III in infarcted animals, but there was no effect of zinc supplementation. Likewise, infarcted animals had higher levels of IL-10, but without zinc interference. Nrf-2 values were not different among the groups. Infarction increased the amount of Treg cells in the spleen as well as the amount of total lymphocytes. Zinc increased the amount of CD4+ in infarcted animals, but we did not observe effects in relation to Treg cells. Conclusion: zinc attenuates cardiac remodeling after infarction in rats; this effect is associated with modulation of antioxidant enzymes, but without the involvement of collagens I and III, Nrf-2, IL-10, and Treg cells.
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spelling Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial InfarctionChronic remodelingCoronary occlusionFunctionHypertrophyTreg cellsZincBackground/Aims: The objective of our study was to evaluate the effects of zinc supplementation on cardiac remodeling following acute myocardial infarction in rats. Methods: Animals were subdivided into 4 groups and observed for 3 months: 1) Sham Control; 2) Sham Zinc: Sham animals receiving zinc supplementation; 3) Infarction Control; 4) Infarction Zinc. After the followup period, we studied hypertrophy and ventricular geometry, functional alterations in vivo and in vitro, changes related to collagen, oxidative stress, and inflammation, assessed by echocardiogram, isolated heart study, western blot, flow cytometer, morphometry, and spectrophotometry. Results: Infarction induced a significant worsening of the functional variables. On the other hand, zinc attenuated both systolic and diastolic cardiac dysfunction induced by infarction. Considering the infarct size, there was no difference between the groups. Catalase and superoxide dismutase decreased in infarcted animals, and zinc increased its activity. We found higher expression of collagens I and III in infarcted animals, but there was no effect of zinc supplementation. Likewise, infarcted animals had higher levels of IL-10, but without zinc interference. Nrf-2 values were not different among the groups. Infarction increased the amount of Treg cells in the spleen as well as the amount of total lymphocytes. Zinc increased the amount of CD4+ in infarcted animals, but we did not observe effects in relation to Treg cells. Conclusion: zinc attenuates cardiac remodeling after infarction in rats; this effect is associated with modulation of antioxidant enzymes, but without the involvement of collagens I and III, Nrf-2, IL-10, and Treg cells.São Paulo State University (Unesp) Botucatu Medical School Internal Medicine DepartmentSão Paulo State University (Unesp) Institute of Biosciences of Botucatu Chemistry and Biochemistry DepartmentSão Paulo State University (Unesp) Institute of Biosciences of Botucatu Microbiology and Immunology DepartmentFlow Citometry Laboratory Amaral Carvalho FundationSão Paulo State University (Unesp) Botucatu Medical School Internal Medicine DepartmentSão Paulo State University (Unesp) Institute of Biosciences of Botucatu Chemistry and Biochemistry DepartmentSão Paulo State University (Unesp) Institute of Biosciences of Botucatu Microbiology and Immunology DepartmentUniversidade Estadual Paulista (Unesp)Amaral Carvalho FundationGonçalves, Andrea F. [UNESP]Polegato, Bertha F. [UNESP]Fernandes, Ana Angélica [UNESP]Ishikawa, Larissa Lumi [UNESP]Okoshi, Katashi [UNESP]Bazan, Silméia G. Z. [UNESP]Minicucci, Marcos F. [UNESP]Azevedo, Paula S. [UNESP]Ikoma, Maura R.Penitenti, MarcimaraChiuso-Minicucci, Fernanda [UNESP]R Paiva, Sergio A. [UNESP]Zornoff, Leonardo A. M. [UNESP]2019-10-06T15:59:20Z2019-10-06T15:59:20Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article363-377http://dx.doi.org/10.1159/000494011Cellular Physiology and Biochemistry, v. 50, n. 1, p. 363-377, 2018.1421-97781015-8987http://hdl.handle.net/11449/18816510.1159/0004940112-s2.0-85054488500Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2024-08-14T17:23:20Zoai:repositorio.unesp.br:11449/188165Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
title Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
spellingShingle Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
Gonçalves, Andrea F. [UNESP]
Chronic remodeling
Coronary occlusion
Function
Hypertrophy
Treg cells
Zinc
title_short Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
title_full Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
title_fullStr Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
title_full_unstemmed Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
title_sort Zinc Supplementation Attenuates Cardiac Remodeling after Experimental Myocardial Infarction
author Gonçalves, Andrea F. [UNESP]
author_facet Gonçalves, Andrea F. [UNESP]
Polegato, Bertha F. [UNESP]
Fernandes, Ana Angélica [UNESP]
Ishikawa, Larissa Lumi [UNESP]
Okoshi, Katashi [UNESP]
Bazan, Silméia G. Z. [UNESP]
Minicucci, Marcos F. [UNESP]
Azevedo, Paula S. [UNESP]
Ikoma, Maura R.
Penitenti, Marcimara
Chiuso-Minicucci, Fernanda [UNESP]
R Paiva, Sergio A. [UNESP]
Zornoff, Leonardo A. M. [UNESP]
author_role author
author2 Polegato, Bertha F. [UNESP]
Fernandes, Ana Angélica [UNESP]
Ishikawa, Larissa Lumi [UNESP]
Okoshi, Katashi [UNESP]
Bazan, Silméia G. Z. [UNESP]
Minicucci, Marcos F. [UNESP]
Azevedo, Paula S. [UNESP]
Ikoma, Maura R.
Penitenti, Marcimara
Chiuso-Minicucci, Fernanda [UNESP]
R Paiva, Sergio A. [UNESP]
Zornoff, Leonardo A. M. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Amaral Carvalho Fundation
dc.contributor.author.fl_str_mv Gonçalves, Andrea F. [UNESP]
Polegato, Bertha F. [UNESP]
Fernandes, Ana Angélica [UNESP]
Ishikawa, Larissa Lumi [UNESP]
Okoshi, Katashi [UNESP]
Bazan, Silméia G. Z. [UNESP]
Minicucci, Marcos F. [UNESP]
Azevedo, Paula S. [UNESP]
Ikoma, Maura R.
Penitenti, Marcimara
Chiuso-Minicucci, Fernanda [UNESP]
R Paiva, Sergio A. [UNESP]
Zornoff, Leonardo A. M. [UNESP]
dc.subject.por.fl_str_mv Chronic remodeling
Coronary occlusion
Function
Hypertrophy
Treg cells
Zinc
topic Chronic remodeling
Coronary occlusion
Function
Hypertrophy
Treg cells
Zinc
description Background/Aims: The objective of our study was to evaluate the effects of zinc supplementation on cardiac remodeling following acute myocardial infarction in rats. Methods: Animals were subdivided into 4 groups and observed for 3 months: 1) Sham Control; 2) Sham Zinc: Sham animals receiving zinc supplementation; 3) Infarction Control; 4) Infarction Zinc. After the followup period, we studied hypertrophy and ventricular geometry, functional alterations in vivo and in vitro, changes related to collagen, oxidative stress, and inflammation, assessed by echocardiogram, isolated heart study, western blot, flow cytometer, morphometry, and spectrophotometry. Results: Infarction induced a significant worsening of the functional variables. On the other hand, zinc attenuated both systolic and diastolic cardiac dysfunction induced by infarction. Considering the infarct size, there was no difference between the groups. Catalase and superoxide dismutase decreased in infarcted animals, and zinc increased its activity. We found higher expression of collagens I and III in infarcted animals, but there was no effect of zinc supplementation. Likewise, infarcted animals had higher levels of IL-10, but without zinc interference. Nrf-2 values were not different among the groups. Infarction increased the amount of Treg cells in the spleen as well as the amount of total lymphocytes. Zinc increased the amount of CD4+ in infarcted animals, but we did not observe effects in relation to Treg cells. Conclusion: zinc attenuates cardiac remodeling after infarction in rats; this effect is associated with modulation of antioxidant enzymes, but without the involvement of collagens I and III, Nrf-2, IL-10, and Treg cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
2019-10-06T15:59:20Z
2019-10-06T15:59:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000494011
Cellular Physiology and Biochemistry, v. 50, n. 1, p. 363-377, 2018.
1421-9778
1015-8987
http://hdl.handle.net/11449/188165
10.1159/000494011
2-s2.0-85054488500
url http://dx.doi.org/10.1159/000494011
http://hdl.handle.net/11449/188165
identifier_str_mv Cellular Physiology and Biochemistry, v. 50, n. 1, p. 363-377, 2018.
1421-9778
1015-8987
10.1159/000494011
2-s2.0-85054488500
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 363-377
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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