Toxocariasis: Serological diagnosis by indirect antibody competition elisa

Detalhes bibliográficos
Autor(a) principal: Nunes, Caris Maroni [UNESP]
Data de Publicação: 1999
Outros Autores: Tundisi, Regina Nardini, Heinemann, Marcos Bryan, Ogassawara, Saemi, Richtzenhain, Leonardo José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46651999000200006
http://hdl.handle.net/11449/65970
Resumo: Toxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).
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spelling Toxocariasis: Serological diagnosis by indirect antibody competition elisaImmunodiagnosisIndirect antibody competition ELISAToxocara canisToxocariasisVisceral larva migranshelminth antibodyimmunoglobulin Gparasite antigenanimalbloodenzyme linked immunosorbent assayfemalehumanimmunologymethodologymousesensitivity and specificityserologytoxocariasisAnimalsAntibodies, HelminthAntigens, HelminthEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin GMiceSensitivity and SpecificitySerologic TestsToxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).Curso de Med. Veterinana-UNESP, Rua Clovis Pestana 793, 16050-680 Araçatuba, SPCurso de Med. Veterinana-UNESP, Rua Clovis Pestana 793, 16050-680 Araçatuba, SPUniversidade Estadual Paulista (Unesp)Nunes, Caris Maroni [UNESP]Tundisi, Regina NardiniHeinemann, Marcos BryanOgassawara, SaemiRichtzenhain, Leonardo José2014-05-27T11:19:49Z2014-05-27T11:19:49Z1999-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-100application/pdfhttp://dx.doi.org/10.1590/S0036-46651999000200006Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.0036-4665http://hdl.handle.net/11449/6597010.1590/S0036-46651999000200006S0036-466519990002000062-s2.0-0033091502S0036-46651999000200006.pdf1892359871207408Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-09-04T19:15:10Zoai:repositorio.unesp.br:11449/65970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Toxocariasis: Serological diagnosis by indirect antibody competition elisa
title Toxocariasis: Serological diagnosis by indirect antibody competition elisa
spellingShingle Toxocariasis: Serological diagnosis by indirect antibody competition elisa
Nunes, Caris Maroni [UNESP]
Immunodiagnosis
Indirect antibody competition ELISA
Toxocara canis
Toxocariasis
Visceral larva migrans
helminth antibody
immunoglobulin G
parasite antigen
animal
blood
enzyme linked immunosorbent assay
female
human
immunology
methodology
mouse
sensitivity and specificity
serology
toxocariasis
Animals
Antibodies, Helminth
Antigens, Helminth
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G
Mice
Sensitivity and Specificity
Serologic Tests
title_short Toxocariasis: Serological diagnosis by indirect antibody competition elisa
title_full Toxocariasis: Serological diagnosis by indirect antibody competition elisa
title_fullStr Toxocariasis: Serological diagnosis by indirect antibody competition elisa
title_full_unstemmed Toxocariasis: Serological diagnosis by indirect antibody competition elisa
title_sort Toxocariasis: Serological diagnosis by indirect antibody competition elisa
author Nunes, Caris Maroni [UNESP]
author_facet Nunes, Caris Maroni [UNESP]
Tundisi, Regina Nardini
Heinemann, Marcos Bryan
Ogassawara, Saemi
Richtzenhain, Leonardo José
author_role author
author2 Tundisi, Regina Nardini
Heinemann, Marcos Bryan
Ogassawara, Saemi
Richtzenhain, Leonardo José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nunes, Caris Maroni [UNESP]
Tundisi, Regina Nardini
Heinemann, Marcos Bryan
Ogassawara, Saemi
Richtzenhain, Leonardo José
dc.subject.por.fl_str_mv Immunodiagnosis
Indirect antibody competition ELISA
Toxocara canis
Toxocariasis
Visceral larva migrans
helminth antibody
immunoglobulin G
parasite antigen
animal
blood
enzyme linked immunosorbent assay
female
human
immunology
methodology
mouse
sensitivity and specificity
serology
toxocariasis
Animals
Antibodies, Helminth
Antigens, Helminth
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G
Mice
Sensitivity and Specificity
Serologic Tests
topic Immunodiagnosis
Indirect antibody competition ELISA
Toxocara canis
Toxocariasis
Visceral larva migrans
helminth antibody
immunoglobulin G
parasite antigen
animal
blood
enzyme linked immunosorbent assay
female
human
immunology
methodology
mouse
sensitivity and specificity
serology
toxocariasis
Animals
Antibodies, Helminth
Antigens, Helminth
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G
Mice
Sensitivity and Specificity
Serologic Tests
description Toxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).
publishDate 1999
dc.date.none.fl_str_mv 1999-12-01
2014-05-27T11:19:49Z
2014-05-27T11:19:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46651999000200006
Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.
0036-4665
http://hdl.handle.net/11449/65970
10.1590/S0036-46651999000200006
S0036-46651999000200006
2-s2.0-0033091502
S0036-46651999000200006.pdf
1892359871207408
url http://dx.doi.org/10.1590/S0036-46651999000200006
http://hdl.handle.net/11449/65970
identifier_str_mv Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.
0036-4665
10.1590/S0036-46651999000200006
S0036-46651999000200006
2-s2.0-0033091502
S0036-46651999000200006.pdf
1892359871207408
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
1.489
0,669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 95-100
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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