Toxocariasis: Serological diagnosis by indirect antibody competition elisa
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0036-46651999000200006 http://hdl.handle.net/11449/65970 |
Resumo: | Toxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%). |
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Toxocariasis: Serological diagnosis by indirect antibody competition elisaImmunodiagnosisIndirect antibody competition ELISAToxocara canisToxocariasisVisceral larva migranshelminth antibodyimmunoglobulin Gparasite antigenanimalbloodenzyme linked immunosorbent assayfemalehumanimmunologymethodologymousesensitivity and specificityserologytoxocariasisAnimalsAntibodies, HelminthAntigens, HelminthEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin GMiceSensitivity and SpecificitySerologic TestsToxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%).Curso de Med. Veterinana-UNESP, Rua Clovis Pestana 793, 16050-680 Araçatuba, SPCurso de Med. Veterinana-UNESP, Rua Clovis Pestana 793, 16050-680 Araçatuba, SPUniversidade Estadual Paulista (Unesp)Nunes, Caris Maroni [UNESP]Tundisi, Regina NardiniHeinemann, Marcos BryanOgassawara, SaemiRichtzenhain, Leonardo José2014-05-27T11:19:49Z2014-05-27T11:19:49Z1999-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-100application/pdfhttp://dx.doi.org/10.1590/S0036-46651999000200006Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999.0036-4665http://hdl.handle.net/11449/6597010.1590/S0036-46651999000200006S0036-466519990002000062-s2.0-0033091502S0036-46651999000200006.pdf1892359871207408Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-09-04T19:15:10Zoai:repositorio.unesp.br:11449/65970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
title |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
spellingShingle |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa Nunes, Caris Maroni [UNESP] Immunodiagnosis Indirect antibody competition ELISA Toxocara canis Toxocariasis Visceral larva migrans helminth antibody immunoglobulin G parasite antigen animal blood enzyme linked immunosorbent assay female human immunology methodology mouse sensitivity and specificity serology toxocariasis Animals Antibodies, Helminth Antigens, Helminth Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Sensitivity and Specificity Serologic Tests |
title_short |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
title_full |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
title_fullStr |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
title_full_unstemmed |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
title_sort |
Toxocariasis: Serological diagnosis by indirect antibody competition elisa |
author |
Nunes, Caris Maroni [UNESP] |
author_facet |
Nunes, Caris Maroni [UNESP] Tundisi, Regina Nardini Heinemann, Marcos Bryan Ogassawara, Saemi Richtzenhain, Leonardo José |
author_role |
author |
author2 |
Tundisi, Regina Nardini Heinemann, Marcos Bryan Ogassawara, Saemi Richtzenhain, Leonardo José |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nunes, Caris Maroni [UNESP] Tundisi, Regina Nardini Heinemann, Marcos Bryan Ogassawara, Saemi Richtzenhain, Leonardo José |
dc.subject.por.fl_str_mv |
Immunodiagnosis Indirect antibody competition ELISA Toxocara canis Toxocariasis Visceral larva migrans helminth antibody immunoglobulin G parasite antigen animal blood enzyme linked immunosorbent assay female human immunology methodology mouse sensitivity and specificity serology toxocariasis Animals Antibodies, Helminth Antigens, Helminth Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Sensitivity and Specificity Serologic Tests |
topic |
Immunodiagnosis Indirect antibody competition ELISA Toxocara canis Toxocariasis Visceral larva migrans helminth antibody immunoglobulin G parasite antigen animal blood enzyme linked immunosorbent assay female human immunology methodology mouse sensitivity and specificity serology toxocariasis Animals Antibodies, Helminth Antigens, Helminth Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Sensitivity and Specificity Serologic Tests |
description |
Toxocariasis is caused by infection of man by Toxocara canis and Toxocara, cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody. in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%). |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-12-01 2014-05-27T11:19:49Z 2014-05-27T11:19:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0036-46651999000200006 Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999. 0036-4665 http://hdl.handle.net/11449/65970 10.1590/S0036-46651999000200006 S0036-46651999000200006 2-s2.0-0033091502 S0036-46651999000200006.pdf 1892359871207408 |
url |
http://dx.doi.org/10.1590/S0036-46651999000200006 http://hdl.handle.net/11449/65970 |
identifier_str_mv |
Revista do Instituto de Medicina Tropical de Sao Paulo, v. 41, n. 2, p. 95-100, 1999. 0036-4665 10.1590/S0036-46651999000200006 S0036-46651999000200006 2-s2.0-0033091502 S0036-46651999000200006.pdf 1892359871207408 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo 1.489 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
95-100 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021365555658752 |