Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

Detalhes bibliográficos
Autor(a) principal: Curbete, Mariane MacHado [UNESP]
Data de Publicação: 2016
Outros Autores: Salgado, Hérida Regina Nunes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.talanta.2016.01.069
http://hdl.handle.net/11449/177855
Resumo: Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 μg mL-1. This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive, fast, and more economical.
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spelling Rapid turbidimetric assay for quantification of fusidic acid in a dermatological creamAntibioticFusidic acidQuality controlTurbidimetric assayValidationFusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 μg mL-1. This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive, fast, and more economical.School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista Campus Araraquara Department of Pharmaceuticals, Rodovia Araraquara-Jaú, km 1School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista Campus Araraquara Department of Pharmaceuticals, Rodovia Araraquara-Jaú, km 1Universidade Estadual Paulista (Unesp)Curbete, Mariane MacHado [UNESP]Salgado, Hérida Regina Nunes [UNESP]2018-12-11T17:27:26Z2018-12-11T17:27:26Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-56application/pdfhttp://dx.doi.org/10.1016/j.talanta.2016.01.069Talanta, v. 153, p. 51-56.0039-9140http://hdl.handle.net/11449/17785510.1016/j.talanta.2016.01.0692-s2.0-849594716252-s2.0-84959471625.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTalanta1,186info:eu-repo/semantics/openAccess2024-06-24T13:44:54Zoai:repositorio.unesp.br:11449/177855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:02:49.758119Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
title Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
spellingShingle Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
Curbete, Mariane MacHado [UNESP]
Antibiotic
Fusidic acid
Quality control
Turbidimetric assay
Validation
title_short Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
title_full Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
title_fullStr Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
title_full_unstemmed Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
title_sort Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream
author Curbete, Mariane MacHado [UNESP]
author_facet Curbete, Mariane MacHado [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
author_role author
author2 Salgado, Hérida Regina Nunes [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Curbete, Mariane MacHado [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
dc.subject.por.fl_str_mv Antibiotic
Fusidic acid
Quality control
Turbidimetric assay
Validation
topic Antibiotic
Fusidic acid
Quality control
Turbidimetric assay
Validation
description Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 μg mL-1. This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive, fast, and more economical.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:27:26Z
2018-12-11T17:27:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.talanta.2016.01.069
Talanta, v. 153, p. 51-56.
0039-9140
http://hdl.handle.net/11449/177855
10.1016/j.talanta.2016.01.069
2-s2.0-84959471625
2-s2.0-84959471625.pdf
url http://dx.doi.org/10.1016/j.talanta.2016.01.069
http://hdl.handle.net/11449/177855
identifier_str_mv Talanta, v. 153, p. 51-56.
0039-9140
10.1016/j.talanta.2016.01.069
2-s2.0-84959471625
2-s2.0-84959471625.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Talanta
1,186
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 51-56
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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