Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico

Detalhes bibliográficos
Autor(a) principal: Módolo, Norma Sueli Pinheiro [UNESP]
Data de Publicação: 2000
Outros Autores: Castiglia, Yara Marcondes Machado [UNESP], Ganem, Eliana Marisa [UNESP], Braz, José Reinaldo Cerqueira [UNESP], Vianna, Pedro Thadeu Galvão [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.sba.com.br/arquivos/revista/rba/mai00239.pdf
http://hdl.handle.net/11449/66071
Resumo: Background and Objectives - Allopurinol is a drug which inhibits the formation of noxious renal free radicals. The aim of this study was to evaluate the protecting renal effects of allopurinol in ischemic kidneys of dogs. Methods - Sixteen dogs were anesthetized with sodium pentobarbital and submitted to extracellular volume expansion (1.4 ml.kg-1.min-1), to mechanic ventilation with air, to right nephrectomy and to left renal artery clamping. Changes which might occur in renal morphology and function after 30 min of total ischemia and posterior reperfusion were studied in Group 1 (G1), in addition to the action of allopurinol (50 mg.kg-1) on those kidneys, when administered 24 h before the experiment and 1 h before the ischemic procedure in Group 2 (G2). The following parameters: heart rate, inferior vena cava pressure, mean blood pressure, PAH clearance (PAH(c)), renal blood flow (RBF), renal vascular resistance (RVR), creatinine clearance (Cr(c)), filtration fraction, urine output, plasma and urine osmolality, osmolar clearance, free water, sodium and potassium clearance, urine and fractional sodium and potassium excretion, hematocrit, rectal temperature, and left kidney histology were evaluated in four moments: M1 control, and M2, M3, M4 obtained immediately, 15 and 30 min after unclamping of the left renal artery. In G2, M1, M2, M3 and M4 were obtained 45, 90, 105, and 120 min after the second allopurinol dose. Results - Both groups showed the highest values for PAH(c), RBF, and Cr(c), and the lowest values for RVR in M1. Animals were tachycardiac since the beginning of the experiment both in G1 and in G2. The other parameters were not changed. Left kidney histological evaluation showed alterations compatible with acute tubular necrosis in both experimental groups. Conclusions - Alterations found in renal hemodynamics were compatible with the release of vasoconstrictor substances due to renal ischemia. Allopurinol was not effective in preventing renal alterations caused by ischemia and reperfusion.
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spelling Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodicoEffects of allopurinol in ischemic kidneys of dogs anesthetized with sodium pentobarbitalANIMAL: dogCOMPLICATIONS: renal ischemiaDRUGS: allopurinolHYPNOTICS, Barbiturate: pentobarbitalallopurinolcreatininepentobarbitalpotassiumsodiumanesthesiaanimal experimentartery clampcontrolled studydogdrug effectfemalehematocrithemodynamicskidney clearancekidney ischemiakidney perfusionmalenephrectomynonhumanplasma osmolalityurine osmolalityurine volumeBackground and Objectives - Allopurinol is a drug which inhibits the formation of noxious renal free radicals. The aim of this study was to evaluate the protecting renal effects of allopurinol in ischemic kidneys of dogs. Methods - Sixteen dogs were anesthetized with sodium pentobarbital and submitted to extracellular volume expansion (1.4 ml.kg-1.min-1), to mechanic ventilation with air, to right nephrectomy and to left renal artery clamping. Changes which might occur in renal morphology and function after 30 min of total ischemia and posterior reperfusion were studied in Group 1 (G1), in addition to the action of allopurinol (50 mg.kg-1) on those kidneys, when administered 24 h before the experiment and 1 h before the ischemic procedure in Group 2 (G2). The following parameters: heart rate, inferior vena cava pressure, mean blood pressure, PAH clearance (PAH(c)), renal blood flow (RBF), renal vascular resistance (RVR), creatinine clearance (Cr(c)), filtration fraction, urine output, plasma and urine osmolality, osmolar clearance, free water, sodium and potassium clearance, urine and fractional sodium and potassium excretion, hematocrit, rectal temperature, and left kidney histology were evaluated in four moments: M1 control, and M2, M3, M4 obtained immediately, 15 and 30 min after unclamping of the left renal artery. In G2, M1, M2, M3 and M4 were obtained 45, 90, 105, and 120 min after the second allopurinol dose. Results - Both groups showed the highest values for PAH(c), RBF, and Cr(c), and the lowest values for RVR in M1. Animals were tachycardiac since the beginning of the experiment both in G1 and in G2. The other parameters were not changed. Left kidney histological evaluation showed alterations compatible with acute tubular necrosis in both experimental groups. Conclusions - Alterations found in renal hemodynamics were compatible with the release of vasoconstrictor substances due to renal ischemia. Allopurinol was not effective in preventing renal alterations caused by ischemia and reperfusion.Dept. de Anestesiologia da FMB UNESP, Distrito de Rubiao Junior, 18618-970 Botucatu, SPDept. de Anestesiologia da FMB UNESP, Distrito de Rubiao Junior, 18618-970 Botucatu, SPUniversidade Estadual Paulista (Unesp)Módolo, Norma Sueli Pinheiro [UNESP]Castiglia, Yara Marcondes Machado [UNESP]Ganem, Eliana Marisa [UNESP]Braz, José Reinaldo Cerqueira [UNESP]Vianna, Pedro Thadeu Galvão [UNESP]2014-05-27T11:19:52Z2014-05-27T11:19:52Z2000-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article239-245application/pdfhttp://www.sba.com.br/arquivos/revista/rba/mai00239.pdfRevista Brasileira de Anestesiologia, v. 50, n. 3, p. 239-245, 2000.0034-7094http://hdl.handle.net/11449/660712-s2.0-00340833882-s2.0-0034083388.pdf82235464757240588226942130768820Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Anestesiologia0.8500,320info:eu-repo/semantics/openAccess2024-08-14T13:20:26Zoai:repositorio.unesp.br:11449/66071Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T13:20:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
Effects of allopurinol in ischemic kidneys of dogs anesthetized with sodium pentobarbital
title Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
spellingShingle Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
Módolo, Norma Sueli Pinheiro [UNESP]
ANIMAL: dog
COMPLICATIONS: renal ischemia
DRUGS: allopurinol
HYPNOTICS, Barbiturate: pentobarbital
allopurinol
creatinine
pentobarbital
potassium
sodium
anesthesia
animal experiment
artery clamp
controlled study
dog
drug effect
female
hematocrit
hemodynamics
kidney clearance
kidney ischemia
kidney perfusion
male
nephrectomy
nonhuman
plasma osmolality
urine osmolality
urine volume
title_short Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
title_full Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
title_fullStr Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
title_full_unstemmed Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
title_sort Efeitos do alopurinol em rins isquemicos de caes anestesiados com pentobarbital sodico
author Módolo, Norma Sueli Pinheiro [UNESP]
author_facet Módolo, Norma Sueli Pinheiro [UNESP]
Castiglia, Yara Marcondes Machado [UNESP]
Ganem, Eliana Marisa [UNESP]
Braz, José Reinaldo Cerqueira [UNESP]
Vianna, Pedro Thadeu Galvão [UNESP]
author_role author
author2 Castiglia, Yara Marcondes Machado [UNESP]
Ganem, Eliana Marisa [UNESP]
Braz, José Reinaldo Cerqueira [UNESP]
Vianna, Pedro Thadeu Galvão [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Módolo, Norma Sueli Pinheiro [UNESP]
Castiglia, Yara Marcondes Machado [UNESP]
Ganem, Eliana Marisa [UNESP]
Braz, José Reinaldo Cerqueira [UNESP]
Vianna, Pedro Thadeu Galvão [UNESP]
dc.subject.por.fl_str_mv ANIMAL: dog
COMPLICATIONS: renal ischemia
DRUGS: allopurinol
HYPNOTICS, Barbiturate: pentobarbital
allopurinol
creatinine
pentobarbital
potassium
sodium
anesthesia
animal experiment
artery clamp
controlled study
dog
drug effect
female
hematocrit
hemodynamics
kidney clearance
kidney ischemia
kidney perfusion
male
nephrectomy
nonhuman
plasma osmolality
urine osmolality
urine volume
topic ANIMAL: dog
COMPLICATIONS: renal ischemia
DRUGS: allopurinol
HYPNOTICS, Barbiturate: pentobarbital
allopurinol
creatinine
pentobarbital
potassium
sodium
anesthesia
animal experiment
artery clamp
controlled study
dog
drug effect
female
hematocrit
hemodynamics
kidney clearance
kidney ischemia
kidney perfusion
male
nephrectomy
nonhuman
plasma osmolality
urine osmolality
urine volume
description Background and Objectives - Allopurinol is a drug which inhibits the formation of noxious renal free radicals. The aim of this study was to evaluate the protecting renal effects of allopurinol in ischemic kidneys of dogs. Methods - Sixteen dogs were anesthetized with sodium pentobarbital and submitted to extracellular volume expansion (1.4 ml.kg-1.min-1), to mechanic ventilation with air, to right nephrectomy and to left renal artery clamping. Changes which might occur in renal morphology and function after 30 min of total ischemia and posterior reperfusion were studied in Group 1 (G1), in addition to the action of allopurinol (50 mg.kg-1) on those kidneys, when administered 24 h before the experiment and 1 h before the ischemic procedure in Group 2 (G2). The following parameters: heart rate, inferior vena cava pressure, mean blood pressure, PAH clearance (PAH(c)), renal blood flow (RBF), renal vascular resistance (RVR), creatinine clearance (Cr(c)), filtration fraction, urine output, plasma and urine osmolality, osmolar clearance, free water, sodium and potassium clearance, urine and fractional sodium and potassium excretion, hematocrit, rectal temperature, and left kidney histology were evaluated in four moments: M1 control, and M2, M3, M4 obtained immediately, 15 and 30 min after unclamping of the left renal artery. In G2, M1, M2, M3 and M4 were obtained 45, 90, 105, and 120 min after the second allopurinol dose. Results - Both groups showed the highest values for PAH(c), RBF, and Cr(c), and the lowest values for RVR in M1. Animals were tachycardiac since the beginning of the experiment both in G1 and in G2. The other parameters were not changed. Left kidney histological evaluation showed alterations compatible with acute tubular necrosis in both experimental groups. Conclusions - Alterations found in renal hemodynamics were compatible with the release of vasoconstrictor substances due to renal ischemia. Allopurinol was not effective in preventing renal alterations caused by ischemia and reperfusion.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
2014-05-27T11:19:52Z
2014-05-27T11:19:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.sba.com.br/arquivos/revista/rba/mai00239.pdf
Revista Brasileira de Anestesiologia, v. 50, n. 3, p. 239-245, 2000.
0034-7094
http://hdl.handle.net/11449/66071
2-s2.0-0034083388
2-s2.0-0034083388.pdf
8223546475724058
8226942130768820
url http://www.sba.com.br/arquivos/revista/rba/mai00239.pdf
http://hdl.handle.net/11449/66071
identifier_str_mv Revista Brasileira de Anestesiologia, v. 50, n. 3, p. 239-245, 2000.
0034-7094
2-s2.0-0034083388
2-s2.0-0034083388.pdf
8223546475724058
8226942130768820
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Anestesiologia
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 239-245
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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