Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jdent.2021.103874 http://hdl.handle.net/11449/229891 |
Resumo: | Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP. |
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Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluationAcquired enamel pellicleCaneCPI-5HemoglobinProteomicsStatherinObjective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP.Department of Biological Sciences Bauru School of Dentistry University of São PauloDepartment of Genetics and Evolution São Carlos Federal UniversityDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)Department of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)Universidade de São Paulo (USP)São Carlos Federal UniversityUniversidade Estadual Paulista (UNESP)Araújo, Tamara TeodoroCarvalho, Thamyris SouzaDionizio, AlineDebortolli, Ana Luiza BogazVentura, Talita Mendes OliveiraSouza, Beatriz MartinesFeitosa, Chelsea Maria Vilas BoasBarbosa, Heloisa Aparecida PereiraRibeiro, Caroline Fernanda da SilvaMartini, TatianaTaira, EvenShibao, Priscila Yumi TanakaHenrique-Silva, FlavioMarchetto, Reinaldo [UNESP]Buzalaf, Marília Afonso Rabelo2022-04-29T08:36:23Z2022-04-29T08:36:23Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2021.103874Journal of Dentistry, v. 116.0300-5712http://hdl.handle.net/11449/22989110.1016/j.jdent.2021.1038742-s2.0-85119117048Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2022-04-29T08:36:23Zoai:repositorio.unesp.br:11449/229891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:36:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
title |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
spellingShingle |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation Araújo, Tamara Teodoro Acquired enamel pellicle CaneCPI-5 Hemoglobin Proteomics Statherin |
title_short |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
title_full |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
title_fullStr |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
title_full_unstemmed |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
title_sort |
Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation |
author |
Araújo, Tamara Teodoro |
author_facet |
Araújo, Tamara Teodoro Carvalho, Thamyris Souza Dionizio, Aline Debortolli, Ana Luiza Bogaz Ventura, Talita Mendes Oliveira Souza, Beatriz Martines Feitosa, Chelsea Maria Vilas Boas Barbosa, Heloisa Aparecida Pereira Ribeiro, Caroline Fernanda da Silva Martini, Tatiana Taira, Even Shibao, Priscila Yumi Tanaka Henrique-Silva, Flavio Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author_role |
author |
author2 |
Carvalho, Thamyris Souza Dionizio, Aline Debortolli, Ana Luiza Bogaz Ventura, Talita Mendes Oliveira Souza, Beatriz Martines Feitosa, Chelsea Maria Vilas Boas Barbosa, Heloisa Aparecida Pereira Ribeiro, Caroline Fernanda da Silva Martini, Tatiana Taira, Even Shibao, Priscila Yumi Tanaka Henrique-Silva, Flavio Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) São Carlos Federal University Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Araújo, Tamara Teodoro Carvalho, Thamyris Souza Dionizio, Aline Debortolli, Ana Luiza Bogaz Ventura, Talita Mendes Oliveira Souza, Beatriz Martines Feitosa, Chelsea Maria Vilas Boas Barbosa, Heloisa Aparecida Pereira Ribeiro, Caroline Fernanda da Silva Martini, Tatiana Taira, Even Shibao, Priscila Yumi Tanaka Henrique-Silva, Flavio Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
dc.subject.por.fl_str_mv |
Acquired enamel pellicle CaneCPI-5 Hemoglobin Proteomics Statherin |
topic |
Acquired enamel pellicle CaneCPI-5 Hemoglobin Proteomics Statherin |
description |
Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:36:23Z 2022-04-29T08:36:23Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2021.103874 Journal of Dentistry, v. 116. 0300-5712 http://hdl.handle.net/11449/229891 10.1016/j.jdent.2021.103874 2-s2.0-85119117048 |
url |
http://dx.doi.org/10.1016/j.jdent.2021.103874 http://hdl.handle.net/11449/229891 |
identifier_str_mv |
Journal of Dentistry, v. 116. 0300-5712 10.1016/j.jdent.2021.103874 2-s2.0-85119117048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964626561007616 |