Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000517959 http://hdl.handle.net/11449/231491 |
Resumo: | Changes in the proteomic profile of the acquired enamel pellicle (AEP) formed for 3 min or 2 h after rinsing with a peptide containing the 15 N-terminal residues of statherin, with serines 2 and 3 phosphorylated (StatpSpS), were evaluated. Nine volunteers participated in 2 consecutive days. Each day, after professional tooth cleaning, they rinsed for 1 min with 10 mL of phosphate buffer containing 1.88 × 10-5 M StatpSpS or phosphate buffer only (control). The acquired pellicle formed on enamel after 3 min or 2 h was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. In the 3-min AEP, 19 and 131 proteins were uniquely identified in the StatpSpS and control groups, respectively. Proteins typically found in the AEP were only found in the latter. Only 2 proteins (neutrophil defensins) were increased upon treatment with StatpSpS, while 65 proteins (among which are several typical AEP proteins) were decreased. In the 2-h AEP, 50 and 108 proteins were uniquely found in StatpSpS and control groups, respectively. Hemoglobin subunits and isoforms of keratin were only found in the StatpSpS group, while cystatin-C, cathepsin D, and cathepsin G, isoforms of heat shock 70 and protocadherin were exclusively found in the control group. In addition, 23 proteins were increased upon treatment with StatpSpS, among which are histatin-1, serum albumin, and isoforms of neutrophil defensin and keratin, while 77 were decreased, most of them were typical AEP proteins. In both evaluated periods, rinsing with StatpSpS profoundly changed the proteomic profile of the AEP, which might impact the protective role of this integument against carious or erosive demineralization. This study provides important insights on the dynamics of the protein composition of the AEP along time, after rinsing with a solution containing StatpSpS. |
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Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel PellicleAcquired pellicleEnamelErosive tooth wearProteomicsStatherinChanges in the proteomic profile of the acquired enamel pellicle (AEP) formed for 3 min or 2 h after rinsing with a peptide containing the 15 N-terminal residues of statherin, with serines 2 and 3 phosphorylated (StatpSpS), were evaluated. Nine volunteers participated in 2 consecutive days. Each day, after professional tooth cleaning, they rinsed for 1 min with 10 mL of phosphate buffer containing 1.88 × 10-5 M StatpSpS or phosphate buffer only (control). The acquired pellicle formed on enamel after 3 min or 2 h was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. In the 3-min AEP, 19 and 131 proteins were uniquely identified in the StatpSpS and control groups, respectively. Proteins typically found in the AEP were only found in the latter. Only 2 proteins (neutrophil defensins) were increased upon treatment with StatpSpS, while 65 proteins (among which are several typical AEP proteins) were decreased. In the 2-h AEP, 50 and 108 proteins were uniquely found in StatpSpS and control groups, respectively. Hemoglobin subunits and isoforms of keratin were only found in the StatpSpS group, while cystatin-C, cathepsin D, and cathepsin G, isoforms of heat shock 70 and protocadherin were exclusively found in the control group. In addition, 23 proteins were increased upon treatment with StatpSpS, among which are histatin-1, serum albumin, and isoforms of neutrophil defensin and keratin, while 77 were decreased, most of them were typical AEP proteins. In both evaluated periods, rinsing with StatpSpS profoundly changed the proteomic profile of the AEP, which might impact the protective role of this integument against carious or erosive demineralization. This study provides important insights on the dynamics of the protein composition of the AEP along time, after rinsing with a solution containing StatpSpS.Department of Biological Sciences Bauru School of Dentistry University of São PauloChemistry Institute Paulista State UniversityChemistry Institute Paulista State UniversityUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Taira, Even AkemiFerrari, Carolina RuisCarvalho, GabrielVentura, Talita Mendes OliveiraMartini, TatianaDionizio, Aline SalgadoAraújo, Tamara TeodoroCrusca, Edson [UNESP]Marchetto, Reinaldo [UNESP]Buzalaf, Marília Afonso Rabelo2022-04-29T08:45:39Z2022-04-29T08:45:39Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article333-340http://dx.doi.org/10.1159/000517959Caries Research, v. 55, n. 4, p. 333-340, 2021.1421-976X0008-6568http://hdl.handle.net/11449/23149110.1159/0005179592-s2.0-85112386340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCaries Researchinfo:eu-repo/semantics/openAccess2024-04-23T15:23:28Zoai:repositorio.unesp.br:11449/231491Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
title |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
spellingShingle |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle Taira, Even Akemi Acquired pellicle Enamel Erosive tooth wear Proteomics Statherin |
title_short |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
title_full |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
title_fullStr |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
title_full_unstemmed |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
title_sort |
Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle |
author |
Taira, Even Akemi |
author_facet |
Taira, Even Akemi Ferrari, Carolina Ruis Carvalho, Gabriel Ventura, Talita Mendes Oliveira Martini, Tatiana Dionizio, Aline Salgado Araújo, Tamara Teodoro Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author_role |
author |
author2 |
Ferrari, Carolina Ruis Carvalho, Gabriel Ventura, Talita Mendes Oliveira Martini, Tatiana Dionizio, Aline Salgado Araújo, Tamara Teodoro Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Taira, Even Akemi Ferrari, Carolina Ruis Carvalho, Gabriel Ventura, Talita Mendes Oliveira Martini, Tatiana Dionizio, Aline Salgado Araújo, Tamara Teodoro Crusca, Edson [UNESP] Marchetto, Reinaldo [UNESP] Buzalaf, Marília Afonso Rabelo |
dc.subject.por.fl_str_mv |
Acquired pellicle Enamel Erosive tooth wear Proteomics Statherin |
topic |
Acquired pellicle Enamel Erosive tooth wear Proteomics Statherin |
description |
Changes in the proteomic profile of the acquired enamel pellicle (AEP) formed for 3 min or 2 h after rinsing with a peptide containing the 15 N-terminal residues of statherin, with serines 2 and 3 phosphorylated (StatpSpS), were evaluated. Nine volunteers participated in 2 consecutive days. Each day, after professional tooth cleaning, they rinsed for 1 min with 10 mL of phosphate buffer containing 1.88 × 10-5 M StatpSpS or phosphate buffer only (control). The acquired pellicle formed on enamel after 3 min or 2 h was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. In the 3-min AEP, 19 and 131 proteins were uniquely identified in the StatpSpS and control groups, respectively. Proteins typically found in the AEP were only found in the latter. Only 2 proteins (neutrophil defensins) were increased upon treatment with StatpSpS, while 65 proteins (among which are several typical AEP proteins) were decreased. In the 2-h AEP, 50 and 108 proteins were uniquely found in StatpSpS and control groups, respectively. Hemoglobin subunits and isoforms of keratin were only found in the StatpSpS group, while cystatin-C, cathepsin D, and cathepsin G, isoforms of heat shock 70 and protocadherin were exclusively found in the control group. In addition, 23 proteins were increased upon treatment with StatpSpS, among which are histatin-1, serum albumin, and isoforms of neutrophil defensin and keratin, while 77 were decreased, most of them were typical AEP proteins. In both evaluated periods, rinsing with StatpSpS profoundly changed the proteomic profile of the AEP, which might impact the protective role of this integument against carious or erosive demineralization. This study provides important insights on the dynamics of the protein composition of the AEP along time, after rinsing with a solution containing StatpSpS. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-04-29T08:45:39Z 2022-04-29T08:45:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000517959 Caries Research, v. 55, n. 4, p. 333-340, 2021. 1421-976X 0008-6568 http://hdl.handle.net/11449/231491 10.1159/000517959 2-s2.0-85112386340 |
url |
http://dx.doi.org/10.1159/000517959 http://hdl.handle.net/11449/231491 |
identifier_str_mv |
Caries Research, v. 55, n. 4, p. 333-340, 2021. 1421-976X 0008-6568 10.1159/000517959 2-s2.0-85112386340 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Caries Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
333-340 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789716353908736 |