Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40659-015-0026-7 http://hdl.handle.net/11449/131344 |
Resumo: | Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididymal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogenate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxidation indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin. |
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Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin EGossypolFertilityOxidative stressBioenergeticsAntioxidant systemVitamin EGossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididymal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogenate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxidation indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Laboratory of Metabolic and Toxicological Biochemistry, Universidade Estadual Paulista (UNESP), Rod. Comandante João Ribeiro de Barros (SP-294), km 651, Dracena, SP, 17900-000, BrazilUniversidade Estadual Paulista, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Tecnológicas de DracenaFAPESP: 2012/22684-6FAPESP: 2012/08668-8FUNDUNESP: 9108913/CDCSantana et al.Universidade Estadual Paulista (Unesp)Santana, Andréia T. [UNESP]Guelfi, Marieli [UNESP]Medeiros, Hyllana C. D. [UNESP]Tavares, Marco A. [UNESP]Bizerra, Paulo F. V. [UNESP]Mingatto, Fábio E. [UNESP]2015-12-07T15:34:08Z2015-12-07T15:34:08Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1186/s40659-015-0026-7Biological Research, v. 48, p. 43-50, 2015.0717-6287http://hdl.handle.net/11449/13134410.1186/s40659-015-0026-7PMC4521381.pdf26227499PMC4521381PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Research0,654info:eu-repo/semantics/openAccess2024-05-07T13:47:03Zoai:repositorio.unesp.br:11449/131344Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:47:03.282485Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
title |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
spellingShingle |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E Santana, Andréia T. [UNESP] Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E |
title_short |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
title_full |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
title_fullStr |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
title_full_unstemmed |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
title_sort |
Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E |
author |
Santana, Andréia T. [UNESP] |
author_facet |
Santana, Andréia T. [UNESP] Guelfi, Marieli [UNESP] Medeiros, Hyllana C. D. [UNESP] Tavares, Marco A. [UNESP] Bizerra, Paulo F. V. [UNESP] Mingatto, Fábio E. [UNESP] |
author_role |
author |
author2 |
Guelfi, Marieli [UNESP] Medeiros, Hyllana C. D. [UNESP] Tavares, Marco A. [UNESP] Bizerra, Paulo F. V. [UNESP] Mingatto, Fábio E. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santana, Andréia T. [UNESP] Guelfi, Marieli [UNESP] Medeiros, Hyllana C. D. [UNESP] Tavares, Marco A. [UNESP] Bizerra, Paulo F. V. [UNESP] Mingatto, Fábio E. [UNESP] |
dc.subject.por.fl_str_mv |
Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E |
topic |
Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E |
description |
Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididymal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogenate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxidation indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:34:08Z 2015-12-07T15:34:08Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40659-015-0026-7 Biological Research, v. 48, p. 43-50, 2015. 0717-6287 http://hdl.handle.net/11449/131344 10.1186/s40659-015-0026-7 PMC4521381.pdf 26227499 PMC4521381 |
url |
http://dx.doi.org/10.1186/s40659-015-0026-7 http://hdl.handle.net/11449/131344 |
identifier_str_mv |
Biological Research, v. 48, p. 43-50, 2015. 0717-6287 10.1186/s40659-015-0026-7 PMC4521381.pdf 26227499 PMC4521381 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Research 0,654 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
43-50 application/pdf |
dc.publisher.none.fl_str_mv |
Santana et al. |
publisher.none.fl_str_mv |
Santana et al. |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128276145635328 |