Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2019v40n6Supl2p3099 http://hdl.handle.net/11449/199879 |
Resumo: | There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg-1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL-1 in total aerobes and 7 × 101 CFU mL-1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL-1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection. |
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Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solutionAnatomyAnimalBacteriumMicrobiological countingSurgeryThere are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg-1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL-1 in total aerobes and 7 × 101 CFU mL-1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL-1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection.Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPPós-Graduando em Cirurgia Veterinária UNESPDepartamento de Patologia Veterinária UNESPDepartamento de Morfologia e Fisiologia Animal UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPPós-Graduando em Cirurgia Veterinária UNESPDepartamento de Patologia Veterinária UNESPDepartamento de Morfologia e Fisiologia Animal UNESPUniversidade Estadual Paulista (Unesp)Pereira, Natália [UNESP]Cardozo, Marita Vedovelli [UNESP]De Sá Rocha, Thiago André Salvitti [UNESP]Zero, Raphael Chiarelo [UNESP]De Ávila, Fernando Antônio [UNESP]De Oliveira, Fabrício Singaretti [UNESP]2020-12-12T01:51:48Z2020-12-12T01:51:48Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3099-3105http://dx.doi.org/10.5433/1679-0359.2019v40n6Supl2p3099Semina:Ciencias Agrarias, v. 40, n. 6, p. 3099-3105, 2019.1679-03591676-546Xhttp://hdl.handle.net/11449/19987910.5433/1679-0359.2019v40n6Supl2p30992-s2.0-8507717273411855642114387880000-0003-1860-8623Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2024-06-07T13:02:27Zoai:repositorio.unesp.br:11449/199879Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:23:35.249805Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
spellingShingle |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution Pereira, Natália [UNESP] Anatomy Animal Bacterium Microbiological counting Surgery |
title_short |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_full |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_fullStr |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_full_unstemmed |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_sort |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
author |
Pereira, Natália [UNESP] |
author_facet |
Pereira, Natália [UNESP] Cardozo, Marita Vedovelli [UNESP] De Sá Rocha, Thiago André Salvitti [UNESP] Zero, Raphael Chiarelo [UNESP] De Ávila, Fernando Antônio [UNESP] De Oliveira, Fabrício Singaretti [UNESP] |
author_role |
author |
author2 |
Cardozo, Marita Vedovelli [UNESP] De Sá Rocha, Thiago André Salvitti [UNESP] Zero, Raphael Chiarelo [UNESP] De Ávila, Fernando Antônio [UNESP] De Oliveira, Fabrício Singaretti [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pereira, Natália [UNESP] Cardozo, Marita Vedovelli [UNESP] De Sá Rocha, Thiago André Salvitti [UNESP] Zero, Raphael Chiarelo [UNESP] De Ávila, Fernando Antônio [UNESP] De Oliveira, Fabrício Singaretti [UNESP] |
dc.subject.por.fl_str_mv |
Anatomy Animal Bacterium Microbiological counting Surgery |
topic |
Anatomy Animal Bacterium Microbiological counting Surgery |
description |
There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg-1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL-1 in total aerobes and 7 × 101 CFU mL-1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL-1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-12T01:51:48Z 2020-12-12T01:51:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2019v40n6Supl2p3099 Semina:Ciencias Agrarias, v. 40, n. 6, p. 3099-3105, 2019. 1679-0359 1676-546X http://hdl.handle.net/11449/199879 10.5433/1679-0359.2019v40n6Supl2p3099 2-s2.0-85077172734 1185564211438788 0000-0003-1860-8623 |
url |
http://dx.doi.org/10.5433/1679-0359.2019v40n6Supl2p3099 http://hdl.handle.net/11449/199879 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 40, n. 6, p. 3099-3105, 2019. 1679-0359 1676-546X 10.5433/1679-0359.2019v40n6Supl2p3099 2-s2.0-85077172734 1185564211438788 0000-0003-1860-8623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina:Ciencias Agrarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3099-3105 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129062165544960 |