Associação do plasma rico em plaquetas e células tronco-mesenquimais na regeneração óssea: uma revisão de literatura

• Autor(a) principal: Mello, Caroline Cantieri de [UNESP]
• Data de Publicação: 2011
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149367; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-26/000844884.pdf
• topic: Regeneração óssea; Plaquetas; Células-tronco; Bone regeneration

The wound healing is a complex and growing area that deals with many cell types and growth factors (GFs). Platelets play important roles not only in hemostasis but also in regenerating damaged tissue. Platelets are an easily accessible, inexpensive, and safe source of GFs. The Platelet-rich Plasma (PRP) has been used as a source of GFs and as a scaffold in bone regeneration. It is autologous, inherently safe and free from transmissible diseases or antigenic reactions development. The rationale for the local use of PRP is the increased levels of GFs in the wound site after platelet degranulation. Due to the increased concentration and release of GFs, PRP can potentially enhance the recruitment and proliferation of stem cells, and endothelial cells. In the field of tissue engineering, therapy with mesenchymal stem cells (MSCs) has been widely investigated in several studies. MSCs are multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate into lineages of mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Some in vitro studies have demonstrated the influence of PRP on MSCs. Lucarelli et al. (2003) evaluated the effects of PRP on human MSCs and observed a significant increase in the number of these cells in the third, sixth and ninth days of the experiment when they were treated with 10% PRP. One potential advantage of this MSCs-PRP mixture is that it is simultaneously osteogenic, osteoconductive and osteoinductive due to the bone forming capacity of MSCs and the presence of secreted GFs in the three-dimensional fibrin scaffold. Additionally, this MSCs-PRP mixture is autologous, non-toxic and exhibits excellent plasticity. The aim of this study was to review current evidence supporting the use of the MSCs-PRP association for the enhancement of bone regeneration, as well...