Ação da própolis sobre a maturação e função de células dendríticas humanas

• Autor(a) principal: Conti, Bruno Jose [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/144028; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/31-08-2016/000869156.pdf
• topic: Própole - Uso terapêutico; Citocinas; Células dendríticas; Resposta imune; Propolis; Dendritic cells

Dendritic cells (DCs) represent a heterogeneous population of professional antigen presenting cells (APCs) and are essential for recognition and presentation of pathogens to T cells. Propolis, a resinous material produced by bees from various plant sources, exhibits numerous biological properties, highlighting its immunomodulatory action. Here, we assayed the effects of propolis on the maturation and fuction of human DCs, assessing the activation of the transcription factor NF-kB, micro-RNAs and cell markers expression (TLR-4, HLA-DR, CD80, CD86, CD40), cytokines production (TNF-α, IL-6, IL-10, IL-12), and the bactericidal activity against Streptococcus mutans. DCs were generated from monocytes treated with IL-4 and GM-CSF and incubated with propolis and LPS. NF-κB in nuclear extract and cytokines were determined by ELISA. microRNAs expression was analysed by RT-qPCR and cell markers by flow cytometry. Colony-forming units were counted to assess the bactericidal activity of propolis-treated DCs. Propolis activated human DCs, inducing the NF-kB signaling pathway and TNF-, IL-6 and IL-10 production. The inhibition of hsa-miR-148a and hsa-miR-148b abolished the inhibitory effects on HLA-DR and pro-inflammatory cytokines. The increased expression of hsa-miR-155 may be correlated to the increase in TLR-4 e CD86 expression, maintaining LPS-induced expression of HLA-DR and CD40. Such parameters may be involved in the increased bactericidal activity of DCs against Streptococcus mutans. These studies are particularly important to develop DC-based modulation strategies for the identification of promising sources for drug discovery