Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells

Detalhes bibliográficos
Autor(a) principal: Conti, B. J. [UNESP]
Data de Publicação: 2023
Outros Autores: Santiago, K. B. [UNESP], Cardoso, E. O. [UNESP], Conte, F. L. [UNESP], Golim, M. A. [UNESP], Cruz, M. T., Sforcin, J. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1414-431X2023e12659
http://hdl.handle.net/11449/247205
Resumo: Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells.
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spelling Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cellsCD4+ T cellsDendritic cellsImmunomodulationPropolisDendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista, SPHemocentro de Botucatu Faculdade de Medicina Universidade Estadual Paulista, SPFaculty of Pharmacy Center for Neurosciences and Cellular Biology University of CoimbraDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista, SPHemocentro de Botucatu Faculdade de Medicina Universidade Estadual Paulista, SPFAPESP: 2015/03409-2FAPESP: 2015/03493-3Universidade Estadual Paulista (UNESP)University of CoimbraConti, B. J. [UNESP]Santiago, K. B. [UNESP]Cardoso, E. O. [UNESP]Conte, F. L. [UNESP]Golim, M. A. [UNESP]Cruz, M. T.Sforcin, J. M. [UNESP]2023-07-29T13:09:37Z2023-07-29T13:09:37Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1414-431X2023e12659Brazilian Journal of Medical and Biological Research, v. 56.1414-431X0100-879Xhttp://hdl.handle.net/11449/24720510.1590/1414-431X2023e126592-s2.0-85152978236Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2023-07-29T13:09:37Zoai:repositorio.unesp.br:11449/247205Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:53:15.804005Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
title Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
spellingShingle Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
Conti, B. J. [UNESP]
CD4+ T cells
Dendritic cells
Immunomodulation
Propolis
title_short Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
title_full Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
title_fullStr Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
title_full_unstemmed Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
title_sort Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
author Conti, B. J. [UNESP]
author_facet Conti, B. J. [UNESP]
Santiago, K. B. [UNESP]
Cardoso, E. O. [UNESP]
Conte, F. L. [UNESP]
Golim, M. A. [UNESP]
Cruz, M. T.
Sforcin, J. M. [UNESP]
author_role author
author2 Santiago, K. B. [UNESP]
Cardoso, E. O. [UNESP]
Conte, F. L. [UNESP]
Golim, M. A. [UNESP]
Cruz, M. T.
Sforcin, J. M. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Coimbra
dc.contributor.author.fl_str_mv Conti, B. J. [UNESP]
Santiago, K. B. [UNESP]
Cardoso, E. O. [UNESP]
Conte, F. L. [UNESP]
Golim, M. A. [UNESP]
Cruz, M. T.
Sforcin, J. M. [UNESP]
dc.subject.por.fl_str_mv CD4+ T cells
Dendritic cells
Immunomodulation
Propolis
topic CD4+ T cells
Dendritic cells
Immunomodulation
Propolis
description Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:09:37Z
2023-07-29T13:09:37Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1414-431X2023e12659
Brazilian Journal of Medical and Biological Research, v. 56.
1414-431X
0100-879X
http://hdl.handle.net/11449/247205
10.1590/1414-431X2023e12659
2-s2.0-85152978236
url http://dx.doi.org/10.1590/1414-431X2023e12659
http://hdl.handle.net/11449/247205
identifier_str_mv Brazilian Journal of Medical and Biological Research, v. 56.
1414-431X
0100-879X
10.1590/1414-431X2023e12659
2-s2.0-85152978236
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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