Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1414-431X2023e12659 http://hdl.handle.net/11449/247205 |
Resumo: | Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells. |
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Repositório Institucional da UNESP |
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spelling |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cellsCD4+ T cellsDendritic cellsImmunomodulationPropolisDendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista, SPHemocentro de Botucatu Faculdade de Medicina Universidade Estadual Paulista, SPFaculty of Pharmacy Center for Neurosciences and Cellular Biology University of CoimbraDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista, SPHemocentro de Botucatu Faculdade de Medicina Universidade Estadual Paulista, SPFAPESP: 2015/03409-2FAPESP: 2015/03493-3Universidade Estadual Paulista (UNESP)University of CoimbraConti, B. J. [UNESP]Santiago, K. B. [UNESP]Cardoso, E. O. [UNESP]Conte, F. L. [UNESP]Golim, M. A. [UNESP]Cruz, M. T.Sforcin, J. M. [UNESP]2023-07-29T13:09:37Z2023-07-29T13:09:37Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1414-431X2023e12659Brazilian Journal of Medical and Biological Research, v. 56.1414-431X0100-879Xhttp://hdl.handle.net/11449/24720510.1590/1414-431X2023e126592-s2.0-85152978236Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2023-07-29T13:09:37Zoai:repositorio.unesp.br:11449/247205Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:53:15.804005Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
title |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
spellingShingle |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells Conti, B. J. [UNESP] CD4+ T cells Dendritic cells Immunomodulation Propolis |
title_short |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
title_full |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
title_fullStr |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
title_full_unstemmed |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
title_sort |
Effect of propolis on Th2 and Th17 cells: interplay with EtxB-and LPS-treated dendritic cells |
author |
Conti, B. J. [UNESP] |
author_facet |
Conti, B. J. [UNESP] Santiago, K. B. [UNESP] Cardoso, E. O. [UNESP] Conte, F. L. [UNESP] Golim, M. A. [UNESP] Cruz, M. T. Sforcin, J. M. [UNESP] |
author_role |
author |
author2 |
Santiago, K. B. [UNESP] Cardoso, E. O. [UNESP] Conte, F. L. [UNESP] Golim, M. A. [UNESP] Cruz, M. T. Sforcin, J. M. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Coimbra |
dc.contributor.author.fl_str_mv |
Conti, B. J. [UNESP] Santiago, K. B. [UNESP] Cardoso, E. O. [UNESP] Conte, F. L. [UNESP] Golim, M. A. [UNESP] Cruz, M. T. Sforcin, J. M. [UNESP] |
dc.subject.por.fl_str_mv |
CD4+ T cells Dendritic cells Immunomodulation Propolis |
topic |
CD4+ T cells Dendritic cells Immunomodulation Propolis |
description |
Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:09:37Z 2023-07-29T13:09:37Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X2023e12659 Brazilian Journal of Medical and Biological Research, v. 56. 1414-431X 0100-879X http://hdl.handle.net/11449/247205 10.1590/1414-431X2023e12659 2-s2.0-85152978236 |
url |
http://dx.doi.org/10.1590/1414-431X2023e12659 http://hdl.handle.net/11449/247205 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research, v. 56. 1414-431X 0100-879X 10.1590/1414-431X2023e12659 2-s2.0-85152978236 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128871434813440 |